1990
DOI: 10.1128/aem.56.8.2404-2409.1990
|View full text |Cite
|
Sign up to set email alerts
|

Transfer of Isolated Nuclei into Protoplasts of Trichoderma harzianum

Abstract: Protoplasts released from young hyphae of Trichoderma harzianum contained 0 to 10 nuclei per protoplast, and most (about 80%) contained from 4 to 6 nuclei. Most protoplasts were larger than 3 ,um in diameter. Nuclei were isolated from protoplasts of an auxotrophic mutant of T. harzianum and transferred into protoplasts obtained from another auxotroph of the same strain. This intrastrain nuclear transfer gave rise to numerous progeny which were stable, prototrophic, and heterokaryotic. Interstrain transfers in … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
2
0
2

Year Published

1991
1991
2003
2003

Publication Types

Select...
3
3
2

Relationship

0
8

Authors

Journals

citations
Cited by 21 publications
(4 citation statements)
references
References 22 publications
0
2
0
2
Order By: Relevance
“…Stasz and Harman (12) studied progenies obtained from many crossings involving different strains from different species of Trichoderma and also observed a very slow development of the recombinant colonies on selective media. Several studies of crossings between strains, within specie or between different species of Trichoderma, through hyphal anastomosis, protoplast fusion or transfer of isolated nuclei into protoplasts, have described the obtainment of unstable recombinants colonies of slow growth on selective media, and with a frequent sector formation (1,3,5,9,10,11,12).…”
Section: Crossings and Recombinant Progeny Analysismentioning
confidence: 99%
“…Stasz and Harman (12) studied progenies obtained from many crossings involving different strains from different species of Trichoderma and also observed a very slow development of the recombinant colonies on selective media. Several studies of crossings between strains, within specie or between different species of Trichoderma, through hyphal anastomosis, protoplast fusion or transfer of isolated nuclei into protoplasts, have described the obtainment of unstable recombinants colonies of slow growth on selective media, and with a frequent sector formation (1,3,5,9,10,11,12).…”
Section: Crossings and Recombinant Progeny Analysismentioning
confidence: 99%
“…Extractions and colorimetric assays of these macromolecules were carried out as described in MATERIALS AND MEXHODS. Because of the low amount of contamination and the good state of the isolated nuclei, the described protocol is potentially useful for studies on fungal nuclei, including genetic transfer by karyoduction [21,22].…”
Section: Resultsmentioning
confidence: 99%
“…interespecíficos (Bagagli et al, 1995;Furlaneto & Pizzirani-Kleiner, 1992;Pecchia & Anné, 1989;Pe'er & Chet, 1990;Silva & Pizzirani-Kleiner, 1999;Sivan et al, 1990;Stasz et al, 1988;Stasz et al, 1989e Stasz & Harman, 1990).…”
Section: Anastomose De Hifas: Incompatibilidade Vegetativa E Observaçunclassified
“…Alguns autores têm descrito análises de recombinantes instáveis de Trichoderma (Bagagli et al, 1995;Pe`er & Chet, 1990;Sivan et al, 1990;Stasz et al, 1988;Stasz et al, 1989; Figura 12 -Reamplificação de bandas de DNA (setas) isoladas dos géis de agarose das amplificações de RAPD (item 4.4). Com exceção da figura (e), todas as reações de reamplificação foram feitas a partir de bandas isoladas de reações de amplificação do DNA da linhagem parental 12L, no caso da figura (e) foi utilizada uma reação de amplificação do DNA da linhagem recombinante 18R.…”
Section: Recombinantesunclassified