Protoplast fusion between Lactobacillus casei and Lactobacillus acidophilus

Yeeh, Yeehn; Jo, Young Bae; Kwon, Oh Chang

doi:10.1007/bf00127893

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Determination of cell surface hydrophobicity. Cell surface hydrophobicity was determined as previously described by Yeeh et al 15) with minor revision. Briefly, the 8 h (stationary phase) test culture was harvested after centrifugation at 4000 × g for 10 min, washed twice, and resuspended in PBS to an optical density (OD 560 ) of 0.4 (A 0 ).…”

Section: Genome Shufflingmentioning

confidence: 99%

Genome shuffling of Lactobacillus plantarum C88 improves adhesion

Zhao

Duan

Gao

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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Genome shuffling is an important method for rapid improvement in microbial strains for desired phenotypes. In this study, ultraviolet irradiation and nitrosoguanidine were used as mutagens to enhance the adhesion of the wild-type Lactobacillus plantarum C88. Four strains with better property were screened after mutagenesis to develop a library of parent strains for three rounds of genome shuffling. Fusants F3-1, F3-2, F3-3, and F3-4 were screened as the improved strains. The in vivo and in vitro tests results indicated that the population after three rounds of genome shuffling exhibited improved adhesive property. Random Amplified Polymorphic DNA results showed significant differences between the parent strain and recombinant strains at DNA level. These results suggest that the adhesive property of L. plantarum C88 can be significantly improved by genome shuffling. Improvement in the adhesive property of bacterial cells by genome shuffling enhances the colonization of probiotic strains which further benefits to exist probiotic function.

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Section: Genome Shufflingmentioning

confidence: 99%

Genome shuffling of Lactobacillus plantarum C88 improves adhesion

Zhao

Duan

Gao

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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“…Protoplast fusion in lactobacilli [8,9] has been reported. However, this technique has been applicable to certain strains of lactobacilli mainly due to the difficulty in both protoplast isolation and regeneration.…”

Section: Introductionmentioning

confidence: 98%

Protoplast formation and regeneration in Lactobacillus delbrueckii

Singhvi¹,

Joshi²,

Gaikaiwari³

et al. 2010

Indian J Microbiol

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Method for production and regeneration of Lactobacillus delbrueckii protoplasts are described. The protoplasts were obtained by treatment with a mixture of lysozyme and mutanolysin in protoplast buffer at pH 6.5 with different osmotic stabilizers. The protoplasts were regenerated on deMan, Rogosa and Sharpe (MRS) with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with sucrose as an osmotic stabilizer using a combination of lysozyme (1 mg/ml) and mutanolysin (10 µg/ml). Maximum protoplast regeneration was obtained on MRS medium with sucrose (0.5 M) as an osmotic stabilizer. The regeneration medium was also applicable to other species of lactobacilli as well. This is, to our knowledge, the first report on protoplast formation and efficient regeneration in case of L. delbrueckii.

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Protoplast fusion between Lactobacillus casei and Lactobacillus acidophilus

Cited by 2 publications

References 15 publications

Genome shuffling of Lactobacillus plantarum C88 improves adhesion

Genome shuffling of Lactobacillus plantarum C88 improves adhesion

Protoplast formation and regeneration in Lactobacillus delbrueckii

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