Protonation of a Spherical Macrotricyclic Tetramine: Water Inclusion, Allosteric Effect, and Cooperativity

Abstract

The spherical macrotricyclic cryptand tetramine “C24” (1) displays remarkable protonation behaviour. It undergoes protonation in four successive steps for which pKa values of 11.17±0.05, 10.28±0.04, 6.00±0.06 and 3.08±0.08 have been determined at 298 K. The unusually close values for the first two protonations provide evidence for the encapsulation of a water molecule serving as effector for the second protonation, which is consistent with earlier observations that the exchange of protons bound in the diprotonated species with solvent protons is unusually slow and that 17O NMR spectra show the presence of an oxygen centre in the same species quite distinct from that of solvent water. Encapsulation of water is also observed in the solid state in the picrate salt of the triprotonated form of 1 and has been characterised by means of X‐ray structural determination.

Exploring the use of rigid 18-membered macrocycles with amide pendant arms for Pb(ii)-based radiopharmaceuticals

“…The experimental protonation constants yield Δlog K 4,5 = 0.62, log K 5,6 = 0.33 and log K 6,7 = 0.67, in reasonable agreement with the values expected according to the statistical factor. 75,76…”

“…The experimental protonation constants yield Δlog K 4,5 = 0.62, log K 5,6 = 0.33 and log K 6,7 = 0.67, in reasonable agreement with the values expected according to the statistical factor. 75,76 The same combined methodology was used to determine the protonation constants of CHX-PYTAMGly 4− . The constants involving the protonation of amine groups, log K 1 and log K 2 , are about 2 log K units higher than those determined for PYTAMGly 4− (Table 2).…”