Proton resonance imaging and relaxation in raw and cooked hen eggs

Jayasundar, Rama; Ayyar, Savita; Raghunathan, P.

doi:10.1016/s0730-725x(97)00010-6

Cited by 13 publications

(9 citation statements)

References 24 publications

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“…These results for T 1 and T 2 values agrees well with a previous report at the same field strength (4.7T) [16]. The shorter T 1 and T 2 values in the fresh egg yolk tentatively attributed to its large lipid content.…”

Section: Resultssupporting

confidence: 92%

“…Interestingly, a fresh hen egg happens to present a big cytoplasm compartment (egg white) that contains a high protein concentration. In addition, the fresh and cooked egg whites have often been used as a phantom to analyze changes in MRI properties of biological tissues [16,17]. In this paper, by comparing the APT effects in a fresh egg white with those in a fresh egg yolk and a cooked egg white, we further postulate that the APT signals measured in vivo do, indeed, originate predominantly from endogenous mobile proteins, such as those in the cytoplasm.…”

Section: Introductionmentioning

confidence: 82%

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A Simple Model for Understanding the Origin of the Amide Proton Transfer MRI Signal in Tissue

2012

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Amide proton transfer (APT) imaging is a new molecular MRI technique that gives contrast at the cellular protein level. To better understand the origin of the APT signal in tissue, fresh and cooked hen eggs (n = 4) were imaged at 4.7 Tesla. The APT effect was quantified using the asymmetry in the magnetization transfer ratio (MTRasym) at the composite amide proton resonance frequency (3.5 ppm from the water resonance). The measured APT signals were significantly higher in the fresh egg white (20.1% ± 0.9%) than in the fresh egg yolk (−1.4% ± 1.1%; P < 0.001), and in the cooked egg white (2.8% ± 0.7%; P < 0.001), all of which have similar absolute protein contents. The data support the notion that the APT effect observed in vivo is associated with mobile proteins in tissue, such as those in the cytoplasm.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 82%

A Simple Model for Understanding the Origin of the Amide Proton Transfer MRI Signal in Tissue

2012

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“…Curiously, signals at chemical shifts that are not typical of yolk lipids are visible. This indication seems, at first sight, in contradiction with the previous NMR spectroscopic studies of intact Xenopus laevis ova13, where yolk lipids explain all the spectroscopic features, which are essentially identical to those of the yolk of an hen egg31. In order to detect metabolites in these samples it was indeed necessary the use of magic angle spinning probes at 14 T loaded with more than one ovum13.…”

Section: Discussioncontrasting

confidence: 58%

NMR spectroscopy of single sub-nL ova with inductive ultra-compact single-chip probes

Grisi

Vincent

Volpe

et al. 2017

Sci Rep

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Nuclear magnetic resonance (NMR) spectroscopy enables non-invasive chemical studies of intact living matter. However, the use of NMR at the volume scale typical of microorganisms is hindered by sensitivity limitations, and experiments on single intact organisms have so far been limited to entities having volumes larger than 5 nL. Here we show NMR spectroscopy experiments conducted on single intact ova of 0.1 and 0.5 nL (i.e. 10 to 50 times smaller than previously achieved), thereby reaching the relevant volume scale where life development begins for a broad variety of organisms, humans included. Performing experiments with inductive ultra-compact (1 mm2) single-chip NMR probes, consisting of a low noise transceiver and a multilayer 150 μm planar microcoil, we demonstrate that the achieved limit of detection (about 5 pmol of 1H nuclei) is sufficient to detect endogenous compounds. Our findings suggest that single-chip probes are promising candidates to enable NMR-based study and selection of microscopic entities at biologically relevant volume scales.

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“…Also it could not detect the blood vessel after Day 12 in the incubation stage, because the developing organs block the propagating light. Duce et al have presented three dimensional high resolution images of the development of avian embryo using micro-magnetic resonance imaging (micro-MRI) from Day 0 to 8 [2][3][4][5][6]. However, micro-MRI has limitations such as relatively slow imaging speed, expensive system, and the difficulty to use in poultry industry.…”

Section: Introductionmentioning

confidence: 99%

Measurement of vital sign in chick embryo using multi-channel diffuse speckle contrast analysis

Yeo

Park

Lee

et al. 2015

2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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In poultry industry which is avian breeding program, the determination whether chick embryos survive in the artificial incubation periods or not is essential to reduce the financial resources. We developed the multi-channel diffuse speckle contrast analysis (DSCA) system composed of four optical fiber detectors enabling to achieve in-vivo measurements of deep tissue flow noninvasively. The system could confirm vital sign of the chick embryo in early incubation stage. Moreover, it demonstrates the change of relative blood flow index and depth information with simplicity, low cost, and flexibility.

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Proton resonance imaging and relaxation in raw and cooked hen eggs

Cited by 13 publications

References 24 publications

A Simple Model for Understanding the Origin of the Amide Proton Transfer MRI Signal in Tissue

A Simple Model for Understanding the Origin of the Amide Proton Transfer MRI Signal in Tissue

NMR spectroscopy of single sub-nL ova with inductive ultra-compact single-chip probes

Measurement of vital sign in chick embryo using multi-channel diffuse speckle contrast analysis

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