Measurement of vital sign in chick embryo using multi-channel diffuse speckle contrast analysis

Abstract

In poultry industry which is avian breeding program, the determination whether chick embryos survive in the artificial incubation periods or not is essential to reduce the financial resources. We developed the multi-channel diffuse speckle contrast analysis (DSCA) system composed of four optical fiber detectors enabling to achieve in-vivo measurements of deep tissue flow noninvasively. The system could confirm vital sign of the chick embryo in early incubation stage. Moreover, it demonstrates the change of relative blood flow index and depth information with simplicity, low cost, and flexibility.

Low frequency oscillations assessed by diffuse speckle contrast analysis for foot angiosome concept

“…Diffuse speckle contrast analysis (DSCA) is an optical methodology that is capable of estimating tissue perfusion changes using correlations in speckle intensities 20 . This system has been recently validated for a variety of biomedical samples [20][21][22][23][24][25] ; it has advantages such as fast signal processing, simple experimental setup and signal analysis, and relatively deep tissue perfusion measurements compared to other blood flow instrumentations. In this study, we employed the DSCA with four-channel optical probes to concurrently obtain LFOs ranging from VLFs to LFs from four sites on the foot, based on the foot angiosome concept.…”