Proton nuclear magnetic resonance lineshape studies on human blood plasma lipids from newborn infants, healthy adults, and adults with tumors

Abstract

The usefulness of proton NMR spectroscopy of human blood plasma for cancer research has been extensively studied in recent years. Two main starting points have been offered by Fossel et al. (N. Engl. J. Med. 315, 1369 (1986)) and Mountford et al. (FEBS Lett. 203, 164 (1986)). In this work the experimental proton NMR spectra of blood plasma were analyzed with the aid of the multivariate lineshape fitting method. An appropriate model structure, in terms of the various lipoprotein (VLDL, LDL, and HDL) signals, for the methylene region was used. Neonates, healthy adults, and adults with nonmalignant and malignant tumors were studied. The linewidth of the methylene region was found to be linearly dependent on the relative concentrations of the lipoproteins. The correlation coefficient was -0.89 (P less than 0.001) for VLDL and 0.88 (P less than 0.001) for HDL. A correlation between VLDL concentration and age, 0.76 (P less than 0.001), was also established. VLDL was modeled using two components. The half-linewidth of the lower field component was slightly elevated for the adults with large metastases. This might be in association with the fucose-containing proteolipid complex detected earlier in cancer cells or in sera of cancer patients. Some signals of this complex may fall in the same region of the spectra. The spectra for the neonates were indicated to be totally different from the adults. This and other related questions were explained by means of the model parameters and the relative concentrations of the lipoproteins VLDL, LDL, and HDL. The presented technique can be used as a rapid research tool for figuring out the relative concentrations of the lipoproteins in blood plasma and explaining the reasons behind the changes in the spectra.

Quantification of plasma lipoprotein fractions by wavelet transform time-domain data processing of the proton nuclear magnetic resonance methylene spectral region

“…Curve fitting, multivariant calibration and neural network methods have already been proposed by J.D. Otvos et al, [7][8][9] Hiltunen et al, [10][11][12] Ala-Korpela et al, [13][14][15][16][17] Engan et al 18 and Bathen et al 19 with promising results.…”

NMR-Spektroskopie – ein modernes Werkzeug zur Serum-Analytik von Lipoproteinen und Metaboliten

“…Wie sich zeigte, war tatsächlich ein erhöhter TGund damit auch VLDL-Spiegel und eine entsprechend geringere HDL-Konzentration -wie sie in gesunden Patienten zu finden ist -der Grund für die schmäleren Linien [38][39][40]. Der Einsatz multivariater Fitmethoden an NMRSpektren ermöglichte hier eine detaillierte Aussage über die relativen Konzentrationen der Lipoproteine (VLDL, LDL und HDL) zueinander [41].…”

NMR spectroscopy – a modern analytical tool for serum analytics of lipoproteins and metabolites

“…As it turned out, the cause of the narrower lines was an increased TG and, thus also, VLDL level as well as a lower HDL concentration -as is found in healthy patients [38 -40] . The application of multivariate fitting methods to NMR spectra allowed for more detailed information about the concentration levels of lipoproteins (VLDL, LDL and HDL) relative to each other [41] .…”