Proton gradient-induced changes of the interaction between CF0 and CF1 as probed by cleavage with NaSCN

Fielder, Heike R.; Пономаренко, С. В.; Gehlen, Nicola von; Strotmann, Heinrich

doi:10.1016/0005-2728(94)90018-3

Cited by 19 publications

(17 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6B). Washing with the chaotropic sodium thiocyanate (NaSCN) removes extrinsic and attached proteins to thylakoid membranes (Fiedler et al, 1994). Thus, protein determination after NaSCN treatment specifically measures only membrane integral complexes.…”

Section: Discussionmentioning

confidence: 99%

“…The eluate was centrifuged at maximum speed (25,000g) with an Eppendorf 5417R table centrifuge. The pellet was then resuspended in distilled water and subjected to a membrane pretreatment (exposure to 5 M NaSCN to bind extrinsic proteins) according to Fiedler et al (1994). Protein concentration was then determined using a standard Lowry assay via Folin-Ciocalteu reagent.…”

Section: Protein Analysismentioning

confidence: 99%

See 1 more Smart Citation

Differential Mobility of Pigment-Protein Complexes in Granal and Agranal Thylakoid Membranes of C3 and C4 Plants

et al. 2012

View full text Add to dashboard Cite

The photosynthetic performance of plants is crucially dependent on the mobility of the molecular complexes that catalyze the conversion of sunlight to metabolic energy equivalents in the thylakoid membrane network inside chloroplasts. The role of the extensive folding of thylakoid membranes leading to structural differentiation into stacked grana regions and unstacked stroma lamellae for diffusion-based processes of the photosynthetic machinery is poorly understood. This study examines, to our knowledge for the first time, the mobility of photosynthetic pigment-protein complexes in unstacked thylakoid regions in the C 3 plant Arabidopsis (Arabidopsis thaliana) and agranal bundle sheath chloroplasts of the C 4 plants sorghum (Sorghum bicolor) and maize (Zea mays) by the fluorescence recovery after photobleaching technique. In unstacked thylakoid membranes, more than 50% of the protein complexes are mobile, whereas this number drops to about 20% in stacked grana regions. The higher molecular mobility in unstacked thylakoid regions is explained by a lower protein-packing density compared with stacked grana regions. It is postulated that thylakoid membrane stacking to form grana leads to protein crowding that impedes lateral diffusion processes but is required for efficient light harvesting of the modularly organized photosystem II and its lightharvesting antenna system. In contrast, the arrangement of the photosystem I light-harvesting complex I in separate units in unstacked thylakoid membranes does not require dense protein packing, which is advantageous for protein diffusion.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Protein Analysismentioning

confidence: 99%

Differential Mobility of Pigment-Protein Complexes in Granal and Agranal Thylakoid Membranes of C3 and C4 Plants

et al. 2012

View full text Add to dashboard Cite

show abstract

“…24 By anion exchange chromatography in the presence of the detergent dodecyl maltoside the resulting subcomplex CF O can be isolated with an intact oligomer III ( Figure 1(A), lane 3). Previous chromatographic methods (e.g.…”

Section: Cf Omentioning

confidence: 98%

Fourteen Protomers Compose the Oligomer III of the Proton-rotor in Spinach Chloroplast ATP Synthase

Seelert

Dencher

Müller

2003

Journal of Molecular Biology

View full text Add to dashboard Cite

“…The vesicles were subsequently dissolved in SDS buffer and subjected to SDS-PAGE. Extraction and colorimetric measurement of Coomassie dye from the excised [5.bands were conducted as described [15]. FoF]-deficient accepter strain RH 304 was completely free of…”

Section: Resultsmentioning

confidence: 99%

Site‐directed mutagenesis of two conserved charged amino acids in the N‐terminal region of α subunit of E. coli‐F₀F₁

et al. 1996

Self Cite

View full text Add to dashboard Cite

Two conserved charged amino acids of the N-terminal 'crown' region of the a subunit of £. coli-Fh a-D36 and a-R40 were exchanged for chemically related (~D36 --, E, a-R40--, K) or unrelated amino acids (a-D36-, K, ~R40--, G), respectively, by employing oligonucleotide-directed mutagenesis. ATP formation and ATP hydrolyzing activity of isolated plasma membrane vesicles was strongly inhibited in mutant HS2 (a-D36--, K), but only slightly affected in the other mutants. The Inhibition is not due to a lower content of FoFI in HS2. In this mutant the extent of the proton gradient generated by ATP hydrolysis was more than 80% Inhibited; in all other transformants much smaller effects were observed. The proton gradient established by NADH oxidation w~,s 33% decreased in HS2, hut was decreased to a lesser extcnt in all other mutants. After blockage of Fo by DCCD treatment, the same NADH-induced proton gradient was obtained in all transformants Including HS2. This and the fact that the activity of NADH oxidation was unchanged indicate increased proton leakiness of FoF1 carrying the a-D36---~K mutation. In F1 ~D36 is located in a domain contacting the subunit in the vicinity of the arginine ~-R52. The effect of a-D36--* K replacement on catalysis and coupling thus may be due to an electrostatic repulsive effect in the crown region which alters the a and [3 subunit interaction.

show abstract

Proton gradient-induced changes of the interaction between CF0 and CF1 as probed by cleavage with NaSCN

Cited by 19 publications

References 44 publications

Differential Mobility of Pigment-Protein Complexes in Granal and Agranal Thylakoid Membranes of C3 and C4 Plants

Differential Mobility of Pigment-Protein Complexes in Granal and Agranal Thylakoid Membranes of C3 and C4 Plants

Fourteen Protomers Compose the Oligomer III of the Proton-rotor in Spinach Chloroplast ATP Synthase

Site‐directed mutagenesis of two conserved charged amino acids in the N‐terminal region of α subunit of E. coli‐F₀F₁

Contact Info

Product

Resources

About

Proton gradient-induced changes of the interaction between CF0 and CF1 as probed by cleavage with NaSCN

Cited by 19 publications

References 44 publications

Differential Mobility of Pigment-Protein Complexes in Granal and Agranal Thylakoid Membranes of C3 and C4 Plants

Differential Mobility of Pigment-Protein Complexes in Granal and Agranal Thylakoid Membranes of C3 and C4 Plants

Fourteen Protomers Compose the Oligomer III of the Proton-rotor in Spinach Chloroplast ATP Synthase

Site‐directed mutagenesis of two conserved charged amino acids in the N‐terminal region of α subunit of E. coli‐F0F1

Contact Info

Product

Resources

About

Site‐directed mutagenesis of two conserved charged amino acids in the N‐terminal region of α subunit of E. coli‐F₀F₁