Protocols for Micropropagation of Woody Trees and Fruits

Jain, S. Mohan; Häggman, Hely

doi:10.1007/978-1-4020-6352-7

Cited by 46 publications

(2 citation statements)

References 81 publications

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“…At maturity, vegetative propagation capacity declines abruptly and silver birch, as well as other tree species with high breeding value, become recalcitrant to propagation methods such as rooting of cuttings, therefore in vitro methods have been investigated (Welander 1993;Jain and Häggman 2007;Bonga 2016). Due to the effect of the in vitro culture environment, it is possible for initially mature plant material to undergo a rejuvenation process via axillary or adventitious shoot proliferation (Fig.…”

Section: Introductionmentioning

confidence: 99%

Expression of juvenility related microRNAs and target genes during micropropagation of silver birch (Betula pendula Roth.)

Krivmane

Girgžde

Samsone

et al. 2022

Plant Cell Tiss Organ Cult

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Silver birch (Betula pendula Roth.) is an ecologically and economically important deciduous tree species in Northern Europe. In vitro culture initiation and shoot rejuvenation from birches in their reproductive phase is difficult and often unsuccessful. Currently, markers to evaluate the degree of juvenility of birch in vitro shoots, which could be used to track the rejuvenation process and to determine factors affecting it, have not been developed. The aims of this study were to assess expression of juvenility related microRNAs and target genes during micropropagation of silver birch, and to investigate factors affecting juvenility of birch genotypes with different in vitro morphogenic ability. Potential precursor sequences and target genes of the microRNAs miR156 and miR172 were determined by alignment to the birch unigene set. Ten potential miR156 and miR172 precursor sequences were identified, and were tested for the ability to form the required stem-loop structure. Based on precursor sequences, primers were designed for real time PCR analysis of precursor miRNA expression. Expression patterns of two miR156 family precursors (miR156_511 and miR156_789) and one miR172 precursor (miR172_1931) and two target genes (BpSPL1 and BpAP2) had the best correlation with juvenility/maturity in the analysed in vitro propagated silver birch samples. Expression patterns of these miRNA precursors and target genes were also investigated in samples cultured under different in vitro conditions. This study provides an initial survey of molecular markers for assessment of phase change in birch in vitro micropropagation.

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Section: Introductionmentioning

confidence: 99%

Expression of juvenility related microRNAs and target genes during micropropagation of silver birch (Betula pendula Roth.)

Krivmane

Girgžde

Samsone

et al. 2022

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Expression of juvenility related microRNAs and target genes during micropropagation of silver birch (Betula pendula Roth).

Krivmane

Girgžde

Samsone

et al. 2022

Preprint

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Silver birch (Betula pendula Roth.) is an ecologically and economically important deciduous tree species in Northern Europe. In vitro culture initiation and shoot rejuvenation from birches in their reproductive phase is difficult and often unsuccessful. Currently, markers to evaluate the degree of juvenility of birch in vitro shoots, which could be used to track the rejuvenation process and to determine factors affecting it, have not been developed. The aims of this study were to assess expression of juvenility related microRNAs and target genes during micropropagation of silver birch, and to investigate factors affecting juvenility of birch genotypes with different in vitro morphogenic ability. Potential precursor sequences and target genes of the microRNAs miR156 and miR172 were determined by alignment to the birch unigene set. Ten potential miR156 and miR172 precursor sequences were identified, and were tested for the ability to form the required stem-loop structure. Based on precursor sequences, primers were designed for real time PCR analysis of precursor miRNA expression. Expression patterns of two miR156 family precursors (miR156_511 and miR156_789) and one miR172 precursor (miR172_1931) and two target genes (BpSPL1 and BpAP2) had the best correlation with juvenility/maturity in in vitro propagated silver birch. Expression patterns of these miRNA precursors and target genes were also investigated in samples cultured under different in vitro conditions. This study provides an initial survey of molecular markers for assessment of phase change in birch in vitro micropropagation.

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Plant Micropropagation

et al. 2010

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Protocols for Micropropagation of Woody Trees and Fruits

Abstract: R. Hasbún. and R. Rodríguez 36. Tissue culture propagation of Mongolian cherry (Prunus fruticosa L.) and Nanking cherry (Prunus tomentosa L.

Cited by 46 publications

References 81 publications

Expression of juvenility related microRNAs and target genes during micropropagation of silver birch (Betula pendula Roth.)

Expression of juvenility related microRNAs and target genes during micropropagation of silver birch (Betula pendula Roth.)

Expression of juvenility related microRNAs and target genes during micropropagation of silver birch (Betula pendula Roth).

Plant Micropropagation

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