Plant Micropropagation

Илиев, Иван; Gajdoov, Alena; Libiakov, Gabriela; Jain, S. Mohan

doi:10.1002/9780470686522.ch1

Cited by 31 publications

(25 citation statements)

References 50 publications

(23 reference statements)

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“…Micropropagation requires only a small explant for the initiation of culture (George and Debergh, 2008). In addition, the plants growing in vitro are kept in a controlled environment, which allows the effective cloning of economically important plants (Iliev et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

et al. 2016

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Micropropagation of Calophyllum brasiliense Cambess. (Clusiaceae) is a way to overcome difficulties in achieving large-scale plant production, given the recalcitrant nature of the seeds, irregular fructification and absence of natural vegetative propagation of the species. Cultures were established using nodal segments 2 cm in length, obtained from 1-2 year old seedlings, maintained in a greenhouse. Mercury chloride and Plant Preservative Mixture™ were used in the surface sterilizing stage, better results being achieved with Plant Preservative Mixture™ incorporation in culture medium, at any concentration. Polyvinylpyrrolidone, activated charcoal, cysteine, ascorbic acid or citric acid were added to the culture medium to avoid oxidation. After 30 days of culture, polyvinylpirrolidone and ascorbic acid gave better results, eliminating oxidation in most explants. For shoot multiplication, benzylaminopurine was used in concentrations of 4.4 and 8.8 µM in Woody Plant Medium, resulting in an average of 4.43 and 4.68 shoots per explant, respectively, after 90 days. Indole-3-butyric acid and α-naphthalene acetic acid were used to induce root formation, reaching a maximum rooting rate of 24% with 20µM α-naphthalene acetic acid. For acclimatization. the rooted plants were transferred to Plantmax  substrate and cultured in a greenhouse, reaching 79% of survival after 30 days and 60% after one year.

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Section: Introductionmentioning

confidence: 99%

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

et al. 2016

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“…However, these methods are time-consuming and can support spread of pathogens (NAJAF-ABADI and HAMIDOGHLI, 2009). Biotechnological methods like micropropagation open up new solutions for breeding and rapid propagation of valuable, virusfree and genetically stable cultivars under controlled in vitro conditions (ILIEV et al, 2010). Numerous protocols for micropropagation of blackberry and raspberry cultivars were described (RUŽIĆ and LAZIĆ, 2006; NAJAF-ABADI and HAMIDOGHLI, 2009; ZAWADZKA and ORLIKOWSKA, 2006; POOTHONG and REED, 2014).…”

Section: Introductionmentioning

confidence: 99%

Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.)

et al. 2018

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The aim of this work was to evaluate shoot multiplication of two blackberry (Rubus fruticosus L.) cultivars 'Black Satin' and 'Loch Ness' on two culture media: Murashige & Skoog (MS) and its modification Murashige & Skoog Van der Salm medium (MS VDS) which differ only in iron source (FeNaEDTA, FeEDDHA, respectively). Statistical analyses showed no significant difference in shoot multiplication between different iron sources for both tested cultivars. For 'Black Satin' it was shown that double concentration of chelates FeNaEDTA and FeEDDHA in culture media negatively affected on shoot growth and multiplication. These results can be very useful for further optimization of micropropagation process for different Rubus cultivars.

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“…Yet degradation studies to evaluate cytokinin stability in typical storage conditions have not been published. Furthermore, the stability during autoclaving of some of these cytokinins has been studied (kinetin in Miller et al 1955 ; zeatin riboside in Miller 1974 ), but most references in the literature are anecdotal with respect to their being (tZ and 2iP in Iliev et al 2010 ) or not being (BA and kinetin in Panaia et al 2011 ) heat-labile. Because of the limited information available on the chemical stability of cytokinins in solution or their physical stability following an autoclave cycle, the present work was undertaken to determine the stability of five widely used adenine-based cytokinins in aqueous solutions: trans-zeatin (tZ), 6-(γ,γ-dimethylallylamino) purine (2iP), kinetin, benzyladenine (BA), and m - topolin.…”

Section: Introductionmentioning

confidence: 99%

Stability of adenine-based cytokinins in aqueous solution

Hart¹,

Keightley

Sappington³

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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Since the isolation of the first cytokinin almost 60 yr ago, cytokinins have become critically important for ornamental and agricultural crops in plant tissue culture. Despite the extensive research on this class of compounds, little information is available on the chemical stability of cytokinins in solution or following an autoclave cycle with Murashige and Skoog (MS) basal medium. This work describes the stability in aqueous solutions of five widely used adenine-based cytokinins: trans-zeatin (tZ), 6-(γ,γ-dimethylallylamino) purine (2iP), kinetin, benzyladenine (BA), and m-topolin. High pressure liquid chromatography (HPLC) and electrospray ionization-mass spectrometry (ESI-MS) were used to quantify and identify their degradation. BA, kinetin, 2iP, and m-topolin were stable at 1.0 mg mL−1 in 0.05 N KOH, with no statistically significant concentration changes (p > 0.05) after 90 d of storage at temperatures of −20°C, 2−6°C, or 25°C. The cytokinin tZ was used as a model compound to evaluate stability under alkaline and acid conditions as well as after repeated freeze-thaw cycles. Trans-zeatin retained >90% of the initial concentration of 1.0 mg mL−1 when dissolved in 0.01 N KOH and stored at −20°C and 2–6°C for 90 d, with only the 2–6°C temperature treatment showing a statistical significant concentration change (p = 0.03). The 1.0 mg mL−1 tZ solution in 0.01 N KOH was stable through six repeated freeze-thaw cycles over 90 d without any significant change in concentration compared to the initial freeze-thaw. Yet, tZ showed highly significant concentration changes when dissolved at 50 mg mL−1 and 0.5 N KOH. All of these adenine-based cytokinins showed exceptional stability following an autoclave cycle at 121°C, 110 kPa for 30 min when in solutions of 1.0 mg mL−1 in 0.05 N KOH, with no significant degradation detected. Trans-zeatin was also found to be stable after one autoclave cycle with 1× MS-basal salts.Electronic supplementary materialThe online version of this article (doi:10.1007/s11627-015-9734-5) contains supplementary material, which is available to authorized users.

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Plant Micropropagation

Cited by 31 publications

References 50 publications

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.)

Stability of adenine-based cytokinins in aqueous solution

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