Proteomics reveals the effect of type I interferon on the pathogenicity of duck hepatitis A virus genotype 3 in Pekin ducks

Liang, Shuang; Xie, Ming; Tang, Jing; Wang, Minghang; Zhang, Dabing; Hou, Shuisheng

doi:10.1016/j.vetmic.2020.108813

Cited by 11 publications

(12 citation statements)

References 27 publications

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“…For digestion, the protein solution was reduced with 5 mM dithiothreitol for 30 min at 56°C and alkylated with 11 mM iodoacetamide for 15 min at room temperature in darkness. The protein sample was then diluted by adding 100 mM TEAB to urea concentration less than 2 M. Finally, trypsin was added at 1:50 trypsin‐to‐protein mass ratio for the first digestion overnight and 1:100 trypsin‐to‐protein mass ratio for a second 4‐h digestion (Liang, Xie et al , 2020).…”

Section: Methodsmentioning

confidence: 99%

Histone acetyltransferase 1 is a succinyltransferase for histones and non‐histones and promotes tumorigenesis

Yang

Yuan

et al. 2020

EMBO Reports

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Section: Methodsmentioning

confidence: 99%

Histone acetyltransferase 1 is a succinyltransferase for histones and non‐histones and promotes tumorigenesis

Yang

Yuan

et al. 2020

EMBO Reports

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“…Our study showed that there was a correlation between NOD1 and DHAV-3 and that the expression of NOD1 was upregulated in susceptible ducks, which was consistent with the study by Vegna 2016. Previous studies also showed that a large number of cytokines and type I interferons are expressed during DHAV-3 infection in ducks ( 33 , 36 , 37 ). These important clues suggest that innate immunity is likely activated during infection with DHAV-3.…”

Section: Discussionmentioning

confidence: 84%

“…These important clues suggest that innate immunity is likely activated during infection with DHAV-3. We hypothesize that DHAV-3 infection likely trigger the NOD1 signaling pathway, causing the type I interferon gene and cytokine expression levels to be significantly upregulated, which resulted in an aggravated inflammatory response and liver damage ( 37 ) and eventually led to the death of ducklings. However, the NOD family has long been thought to be involved in antibacterial responses ( 38 – 40 ).…”

Section: Discussionmentioning

confidence: 99%

NOD1 Is Associated With the Susceptibility of Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3

et al. 2021

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Duck viral hepatitis (DVH) is an acute, highly lethal infectious disease of ducklings that causes huge losses in the duck industry. Duck hepatitis A virus genotype 3 (DHAV-3) has been one of the most prevalent DVH pathogen in the Asian duck industry in recent years. Here, we investigated the genetic basis of the resistance and susceptibility of ducks to DVH by comparing the genomes and transcriptomes of a resistant Pekin duck flock (Z8) and a susceptible Pekin duck flock (SZ7). Our comparative genomic and transcriptomic analyses suggested that NOD1 showed a strong signal of association with DVH susceptibility in ducks. Then, we found that NOD1 showed a significant expression difference between the livers of susceptible and resistant individuals after infection with DHAV-3, with higher expression in the SZ7 flock. Furthermore, suppression and overexpression experiments showed that the number of DHAV-3 genomic copies in primary duck hepatocytes was influenced by the expression level of NOD1. In addition, in situ RNAscope analysis showed that the localization of NOD1 and DHAV-3 in liver cells was consistent. Altogether, our data suggested that NOD1 was likely associated with DHAV-3 susceptibility in ducks, which provides a target for future investigations of the pathogenesis of DVH.

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“…Protein extraction, trypsin digestion, LC-MS (MS/MS) analysis, and the database search were performed with reference to previously reported methods [68][69][70][71][72][73][74], with some modifications. The sample was ground into a cell powder in liquid nitrogen.…”

Section: Protein Extractionmentioning

confidence: 99%

TMT-based quantitative proteomic analysis reveals defense mechanism of wheat against the crown rot pathogen Fusarium pseudograminearum

Qiao

Yang

et al. 2021

BMC Plant Biol

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Background Fusarium crown rot is major disease in wheat. However, the wheat defense mechanisms against this disease remain poorly understood. Results Using tandem mass tag (TMT) quantitative proteomics, we evaluated a disease-susceptible (UC1110) and a disease-tolerant (PI610750) wheat cultivar inoculated with Fusarium pseudograminearum WZ-8A. The morphological and physiological results showed that the average root diameter and malondialdehyde content in the roots of PI610750 decreased 3 days post-inoculation (dpi), while the average number of root tips increased. Root vigor was significantly increased in both cultivars, indicating that the morphological, physiological, and biochemical responses of the roots to disease differed between the two cultivars. TMT analysis showed that 366 differentially expressed proteins (DEPs) were identified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment in the two comparison groups, UC1110_3dpi/UC1110_0dpi (163) and PI610750_3dpi/PI610750_0dpi (203). It may be concluded that phenylpropanoid biosynthesis (8), secondary metabolite biosynthesis (12), linolenic acid metabolites (5), glutathione metabolism (8), plant hormone signal transduction (3), MAPK signaling pathway-plant (4), and photosynthesis (12) contributed to the defense mechanisms in wheat. Protein-protein interaction network analysis showed that the DEPs interacted in both sugar metabolism and photosynthesis pathways. Sixteen genes were validated by real-time quantitative polymerase chain reaction and were found to be consistent with the proteomics data. Conclusion The results provided insight into the molecular mechanisms of the interaction between wheat and F. pseudograminearum.

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Proteomics reveals the effect of type I interferon on the pathogenicity of duck hepatitis A virus genotype 3 in Pekin ducks

Cited by 11 publications

References 27 publications

Histone acetyltransferase 1 is a succinyltransferase for histones and non‐histones and promotes tumorigenesis

Histone acetyltransferase 1 is a succinyltransferase for histones and non‐histones and promotes tumorigenesis

NOD1 Is Associated With the Susceptibility of Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3

TMT-based quantitative proteomic analysis reveals defense mechanism of wheat against the crown rot pathogen Fusarium pseudograminearum

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