Proteomics Reveals A Potential Role of the Perisperm in Starch Remobilization During Sugarbeet Seed Germination

Catusse, Julie; Job, Claudette; Job, Dominique

doi:10.1007/978-94-007-4749-4_2

Cited by 7 publications

(5 citation statements)

References 96 publications

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“…As far as we know, the α-amylase is synthesized de novo in the aleurone layer surrounding the endosperm in the process of seed germination. In contrast, a precursor form of β-amylase is present in seeds in the dry state as a proenzyme devoid of enzyme activity, which is activated during germination after cleavage of a peptide sequence at the C-terminal part of the enzyme [12]. These results suggested that Spd might not only enhance the activities of α-amylase and β-amylase, but also induce the de novo β-amylase synthesis by elevating β-amylase gene expression at the early stage of white clover seed germination under water stress.…”

Section: Resultsmentioning

confidence: 99%

“…The α-amylases are endoenzymes which can produce shorter chains called limit dextrins, glucose, and maltose by breaking α-(1, 4) bonds at random within polysaccharide chains. The β-amylases are exohydrolases that hydrolyze α-(1, 4) bonds in polysaccharides to remove successive maltose units from the non-reducing ends of α-1, 4-linked poly- and oligoglucans until the first α-1, 6-branching point along the substrate molecule is encountered [12]. Soluble sugars and amylase activities have been positively correlated with seedling vigor [7].…”

Section: Introductionmentioning

confidence: 99%

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Exogenous Spermidine Improves Seed Germination of White Clover under Water Stress via Involvement in Starch Metabolism, Antioxidant Defenses and Relevant Gene Expression

et al. 2014

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This study was designed to determine the effect of exogenous spermidine (Spd) (30 μM) on white clover seed germination under water stress induced by polyethylene glycol 6000. Use of seed priming with Spd improved seed germination percentage, germination vigor, germination index, root viability and length, and shortened mean germination time under different water stress conditions. Seedling fresh weight and dry weight also increased significantly in Spd-treated seeds compared with control (seeds primed with distilled water). Improved starch metabolism was considered a possible reason for this seed invigoration, since seeds primed with Spd had significantly increased α-amylase/β-amylase activities, reducing sugar, fructose and glucose content and transcript level of β-amylase gene but not transcript level of α-amylase gene. In addition, the physiological effects of exogenous Spd on improving seeds' tolerance to water deficit during germination were reflected by lower lipid peroxidation levels, better cell membrane stability and significant higher seed vigour index in seedlings. Enhanced antioxidant enzyme activities (superoxide dismutase, peroxidase, catalase and ascorbate peroxidase), ascorbate-glutathione cycle (ASC-GSH cycle) and transcript level of genes encoding antioxidant enzymes induced by exogenous Spd may be one of the critical reasons behind acquired drought tolerance through scavenging of reactive oxygen species (ROS) in OPEN ACCESSMolecules 2014, 19 18004 water-stressed white clover seeds. The results indicate that Spd plays an important function as a stress-protective compound or physiological activator.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exogenous Spermidine Improves Seed Germination of White Clover under Water Stress via Involvement in Starch Metabolism, Antioxidant Defenses and Relevant Gene Expression

et al. 2014

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“…Catusse et al (2012) observed accumulation of α-amylase in the perisperm extracted from intact whole seeds, but not from the isolated perisperm, suggesting that the embryo participates in the accumulation of this starch mobilization enzyme during germination. Thus, the mechanisms that occur in the perisperm of beet seeds appear to be similar to those documented for cereal endosperm.…”

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confidence: 78%

Beet seed priming with growth regulators

Dotto

Silva

2017

SCA

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Seed priming is a technique used to induce metabolic germination processes. Use of growth regulators in seed priming may facilitate increments in physiological processes during seed germination. The objective of this study was to evaluate the effect of priming and growth regulators on beet seed germination. The treatments were cultivar type (Early Wonder, Itapuã and Maravilha) and seed priming technique, which included a control (unconditioned seed) standard hydropriming (water) and a conditioning with salicylic, gibberellic and ascorbic acids, respectively. The treatments were defined by initially testing 0, 1, 2 and 4 mM ascorbic, gibberellic and salicylic acids. Before seed priming, imbibition seed curves were established to determine the optimal conditioning time. After conditioning, the germination, rate of germination, seedling length and seedling dry weight were evaluated. First, the appropriate dosage for conditioning was determined by using a completely randomized experimental design, with four replications per cultivar. Then, a 3 x 5 (cultivar x conditioning technique) factorial design was adopted. Whenever the results were significant by variance analysis, regression analysis was performed. Finally, Tukey's test was used to compare the means at P=0.05. Beet seed priming alters the potential of germination and is influenced by the cultivar and conditioning technique. Pretreatment with 1−2 mM ascorbic, gibberellic or salicylic acids, respectively, promotes beet seed germination, whereas at 1−3 mM, the growth of roots and shoots of beet seedlings is promoted. The most effective techniques to promote germination and growth of sugar beet seedlings were priming with water, salicylic acid or gibberellic acid. Key words: Beta vulgaris. Hydropriming. Gibberellic acid. Salicylic acid. Ascorbic acid. ResumoO condicionamento fisiológico é uma técnica que permite a ativação dos processos metabólicos e fisiológicos na germinação das sementes por meio da utilização de reguladores de crescimento. Desta forma, objetivou-se avaliar o efeito do condicionamento fisiológico de sementes de beterraba com reguladores de crescimento. Foram utilizadas três cultivares: Early Wonder, Itapuã e Maravilha, submetidas a cinco tipos de condicionamentos: testemunha (semente não condicionada) hidrocondicionamento padrão (água), condicionamento com os ácidos salicílico, giberélico e ascórbico. Para definição dos tratamentos, foram testadas doses de 0; 1; 2 e 4 mMol.L -1 dos ácidos. Previamente ao condicionamento, foram realizadas as curvas de embebição das sementes, para determinação do tempo ideal de condicionamento, logo após, as sementes foram avaliadas quanto à porcentagem e velocidade de germinação, comprimento e massa seca de plântulas. O delineamento experimental utilizado foi inteiramente casualizado com quatro repetições, separado para cada cultivar, determinando-se as dosagens adequadas. Na segunda etapa foi adotado esquema fatorial 3 x 5 (cultivares x tipo de condicionamento). Os resultados foram submetidos a análise ...

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“…During seed germination, the α-amylase is produced de novo in a special layer known as aleurone. In contrast, β-amylase exists in dry form in seeds as a precursor lacking catalytic activity, however, it is activated in consequence of peptide sequence cleavage at the C-terminal portion during the process of germination ( Catusse et al, 2012 ). The present study demonstrated that water deficiency significantly inhibited starch degradation in seeds ( Figure 5 ), but seeds primed with DA-6 considerably alleviated drought-induced decline in the process of starch catabolism through enhancing the catalytic activities of α-amylase and β-amylase under drought stress.…”

Section: Discussionmentioning

confidence: 99%

Diethyl Aminoethyl Hexanoate Priming Ameliorates Seed Germination via Involvement in Hormonal Changes, Osmotic Adjustment, and Dehydrins Accumulation in White Clover Under Drought Stress

et al. 2021

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Drought is a serious outcome of climate change reducing the productivity of forage species under arid and semi-arid conditions worldwide. Diethyl aminoethyl hexanoate (DA-6), a novel plant growth regulator, has proven to be involved in the amelioration of critical physiological functions in many agricultural crops under various abiotic stresses, but the role of the DA-6 in improving seed germination has never been investigated under drought stress. The present study was carried out to elucidate the impact of the DA-6 priming on seeds germination of white clover under drought stress. Results showed that seed priming with the DA-6 significantly mitigated the drought-induced reduction in germination percentage, germination vigor, germination index, seed vigor index, root length, shoot length, and fresh weight after 7 days of seed germination. The DA-6 significantly increased the endogenous indole-3-acetic acid, gibberellin, and cytokinin content with marked reduction in abscisic acid content in seedlings under drought stress. In addition, the DA-6 significantly accelerated starch catabolism by enhancing the activities of hydrolases contributing toward enhanced soluble sugars, proline content and ameliorated the antioxidant defense system to enhance the ability of reactive oxygen species scavenging under drought stress. Furthermore, exogenous DA-6 application significantly increased dehydrins accumulation and upregulated transcript levels of genes encoding dehydrins (SK2, Y2SK, or DHNb) during seeds germination under water deficient condition. These findings suggested that the DA-6 mediated seeds germination and drought tolerance associated with changes in endogenous phytohormones resulting in increased starch degradation, osmotic adjustment, antioxidants activity, and dehydrins accumulation during seed germination under water deficient condition.

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Proteomics Reveals A Potential Role of the Perisperm in Starch Remobilization During Sugarbeet Seed Germination

Cited by 7 publications

References 96 publications

Exogenous Spermidine Improves Seed Germination of White Clover under Water Stress via Involvement in Starch Metabolism, Antioxidant Defenses and Relevant Gene Expression

Exogenous Spermidine Improves Seed Germination of White Clover under Water Stress via Involvement in Starch Metabolism, Antioxidant Defenses and Relevant Gene Expression

Beet seed priming with growth regulators

Diethyl Aminoethyl Hexanoate Priming Ameliorates Seed Germination via Involvement in Hormonal Changes, Osmotic Adjustment, and Dehydrins Accumulation in White Clover Under Drought Stress

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