Proteomics progresses in microbial physiology and clinical antimicrobial therapy

Abstract

Clinical microbial identification plays an important role in optimizing the management of infectious diseases and provides diagnostic and therapeutic support for clinical management. Microbial proteomic research is aimed at identifying proteins associated with microbial activity, which has facilitated the discovery of microbial physiology changes and host–pathogen interactions during bacterial infection and antimicrobial therapy. Here, we summarize proteomic-driven progresses of host–microbial pathogen interactions at multiple levels, mass spectrometry-based microbial proteome identification for clinical diagnosis, and antimicrobial therapy. Proteomic technique progresses pave new ways towards effective prevention and drug discovery for microbial-induced infectious diseases.

Options and Limitations in Clinical Investigation of Bacterial Biofilms

“…The heterogeneous, noncrystalline, and insoluble biofilm extracellular matrix is a rather perplexing assembly. Proteomics has provided deeper knowledge by (i) giving insights into the protein profile determinants that regulate host-pathogen interactions as well as the virulence and pathogenicity traits of the menacing bacteria; (ii) answering substantial questions regarding the biofilm antimicrobial resistance phenotype, offering potential alternatives for rational drug design; and (iii) underpinning physiological differences from planktonic species (187)(188)(189). Indeed, the use of proteomics has been substantiated by mass spectrometry (MS) to extend its utility beyond research in the field of diagnostics to clinically oriented infectious disease investigation, with particular emphasis on host-pathogen interactions (190,191).…”

“…Moving one step further, metaproteomics constitutes a cutting-edge tool for the study of multispecies community interactions by means of cooperation and competitiveness and the ways that these relationships shape the microbial consortium. Metaproteomics also offers the ability to elucidate the metabolic signatures of multispecies biofilms, even for the unculturable bacteria that reside within biofilms but cannot be cultivated by classic microbiological means, potentially providing novel tools for drug design (189,193,194).…”

Possibilities of using proteomic analysis in infectiology

“…Так, например, уже имеются результаты исследования чувствительности микроорганизмов к некоторым наиболее распространенным антибиотикам. Протеомные исследования открывают новые пути к разработке методов ýффективной профилактики и созданию лекарств для лечения инфекционных заболеваний, вызванных микроорганизмами [26][27][28].…”

Microbial Proteomics and Their Importance in Medical Microbiology

“…MS-based proteomic approaches have been used in clinical diagnostic procedures, including the characterization, identification, and classification of microorganisms. Matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS) has been broadly adopted by many clinical microbiology laboratories over the past decade (Chen et al, 2017). MALDI-TOF MS is the most emerging tool used to identify microorganisms at species level, even strain differentiation based on molecular signatures (Wieser et al, 2012).…”

“…Microbial infection is a leading cause of death around the world. Most of the infectious diseases are caused by drug-resistant microbes; this may lead to a delay in the administration of microbiologically effective therapy (Chen et al, 2017;Del Chierico et al, 2014). Therefore, exhaustive understanding of microbial physiologies, infection and defense systems, and survival strategies is of great interest in order to actively defeat microbial infection.…”