Proteomics profiling of epithelium-derived exosomes from nasal polyps revealed signaling functions affecting cellular proliferation

Zhou, Min; Tan, Kai Sen; Guan, Weijun; Jiang, Lijie; Deng, Jie; Gao, Wenxiang; Lee, Yew Mun; Xu, Zhaofeng; Luo, Xin; Liu, Chen; Shi, Jianbo; Lai, Yinyan

doi:10.1016/j.rmed.2020.105871

Cited by 24 publications

(38 citation statements)

References 38 publications

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“…Western blot and fluorescence-activated cell sorting analysis can identify known cellular proteins in exosomes prepared from different cell types [74,107,108,135,136]. For unknown cellular proteins, mass spectrometry associated with trypsin digestions can analyze exosomes derived from cells like mast cells, DCs, and enterocytes [2,105,112,121,[137][138][139][140]. These methods have been used to identify cytosolic proteins present in exosomes including actin, tubulin, cytoskeletal components, and actin-binding proteins, as well as Rab and annexins, which are important in intracellular membrane fusions and transport function (Tables 2 and 3).…”

Section: Proteinsmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosomes: Applications in Regenerative Medicine

Hade

Suire

Suo

2021

Cells

242

154

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Exosomes are a type of extracellular vesicles, produced within multivesicular bodies, that are then released into the extracellular space through a merging of the multivesicular body with the plasma membrane. These vesicles are secreted by almost all cell types to aid in a vast array of cellular functions, including intercellular communication, cell differentiation and proliferation, angiogenesis, stress response, and immune signaling. This ability to contribute to several distinct processes is due to the complexity of exosomes, as they carry a multitude of signaling moieties, including proteins, lipids, cell surface receptors, enzymes, cytokines, transcription factors, and nucleic acids. The favorable biological properties of exosomes including biocompatibility, stability, low toxicity, and proficient exchange of molecular cargos make exosomes prime candidates for tissue engineering and regenerative medicine. Exploring the functions and molecular payloads of exosomes can facilitate tissue regeneration therapies and provide mechanistic insight into paracrine modulation of cellular activities. In this review, we summarize the current knowledge of exosome biogenesis, composition, and isolation methods. We also discuss emerging healing properties of exosomes and exosomal cargos, such as microRNAs, in brain injuries, cardiovascular disease, and COVID-19 amongst others. Overall, this review highlights the burgeoning roles and potential applications of exosomes in regenerative medicine.

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Section: Proteinsmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosomes: Applications in Regenerative Medicine

Hade

Suire

Suo

2021

Cells

242

154

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“…These findings show that typing of NLF MVs might help to identify phenotypes of CRS and in distinguishing AERD from CRS with nasal polyps [ 107 ]. It was also observed that, independently of the presence or absence of concomitant asthma, exosomes released by primary human nasal epithelial cells obtained from patients suffering from CRS with nasal polyposis carried proteins influencing cell proliferation pathways and potentially leading to remodeling of the sinonasal mucosa [ 108 ].…”

Section: Extracellular Vesicles and Asthma: Higher Levels Of Organizationmentioning

confidence: 99%

Extracellular Vesicles and Asthma—More Than Just a Co-Existence

Alhamwe

Potaczek

Miethe

et al. 2021

IJMS

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Extracellular vesicles (EVs) are membranous structures, which are secreted by almost every cell type analyzed so far. In addition to their importance for cell-cell communication under physiological conditions, EVs are also released during pathogenesis and mechanistically contribute to this process. Here we summarize their functional relevance in asthma, one of the most common chronic non-communicable diseases. Asthma is a complex persistent inflammatory disorder of the airways characterized by reversible airflow obstruction and, from a long-term perspective, airway remodeling. Overall, mechanistic studies summarized here indicate the importance of different subtypes of EVs and their variable cargoes in the functioning of the pathways underlying asthma, and show some interesting potential for the development of future therapeutic interventions. Association studies in turn demonstrate a good diagnostic potential of EVs in asthma.

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“…37 EVs from nasal polyps were found to have increased proteins associated with cell proliferation and thus remodelling of mucosal membranes. 94 Treatment of human bronchial epithelial (HBE) cells with proinflammatory cytokines, specific to two subsets of asthma, T2 and T17 (T2 treated IL-4 and IL-13, T17 treated IL-17A and TNFα) increased EV release. 95 EVs isolated from a murine model of asthma were found to have increased levels of immune cell-derived miRNA, such as miR-223 and miR-142a.…”

Section: Asthmamentioning

confidence: 99%

Role of extracellular vesicles in chronic lung disease

Trappe

Donnelly

McNally

et al. 2021

Thorax

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To explore the role of extracellular vesicles (EVs) in chronic lung diseases.EVs are emerging as mediators of intercellular communication and possible diagnostic markers of disease. EVs harbour cargo molecules including RNA, lipids and proteins that they transfer to recipient cells. EVs are intercellular communicators within the lung microenvironment. Due to their disease-specific cargoes, EVs have the promise to be all-in-one complex multimodal biomarkers. EVs also have potential as drug carriers in chronic lung disease.Descriptive discussion of key studies of EVs as contributors to disease pathology, as biomarkers and as potential therapies with a focus on chronic obstructive pulmonary disorder (COPD), cystic fibrosis (CF), asthma, idiopathic pulmonary fibrosis and lung cancer.We provide a broad overview of the roles of EV in chronic respiratory disease. Recent advances in profiling EVs have shown their potential as biomarker candidates. Further studies have provided insight into their disease pathology, particularly in inflammatory processes across a spectrum of lung diseases. EVs are on the horizon as new modes of drug delivery and as therapies themselves in cell-based therapeutics.EVs are relatively untapped sources of information in the clinic that can help further detail the full translational nature of chronic lung disorders.

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Proteomics profiling of epithelium-derived exosomes from nasal polyps revealed signaling functions affecting cellular proliferation

Cited by 24 publications

References 38 publications

Mesenchymal Stem Cell-Derived Exosomes: Applications in Regenerative Medicine

Mesenchymal Stem Cell-Derived Exosomes: Applications in Regenerative Medicine

Extracellular Vesicles and Asthma—More Than Just a Co-Existence

Role of extracellular vesicles in chronic lung disease

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