Proteomics of Buccal Cavity Mucus in Female Tilapia Fish (Oreochromis spp.): A Comparison between Parental and Non-Parental Fish

Iq, Koe Chun; Shu‐Chien, Alexander Chong

doi:10.1371/journal.pone.0018555

Cited by 33 publications

(22 citation statements)

References 55 publications

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“…Intact haemoglobin and its fragments isolated from human and Alligator mississippiensis have been shown to possess a broad spectrum of antimicrobial activity (Hodson & Hirsch 1958;Parish, Jiang, Tokiwa, Berova, Nakanishi, McCabe, Zuckerman, Xia & Gabay 2001). (Iq & Shu-Chien 2011), in human cervical mucus (Panicker et al 2010) and in olfactory cleft mucus (D ebat, Eloit, Blon, Sarazin, Henry, Huet, Trotier & Pernollet 2007). Haemoglobin subunits (alpha and beta) have also been identified in the normal buccal cavity mucus in female Oreochromis spp.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of antibacterial activity and innate immune components in skin mucus of Indian major carp,Cirrhinus mrigala

et al. 2015

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The present work has been undertaken to analyse the antibacterial activity and innate immune components in the skin mucus of Indian major carp, Cirrhinus mrigala. Skin mucus was extracted separately in triple-distilled water (TDW), 3% acetic acid (3% AA) or 0.1% trifluoroacetic acid (1% TFA). All mucus extracts exhibited different spectrum of the antibacterial activity against different groups of pathogenic bacteria. Protein profiling by polyacrylamide gel electrophoresis revealed a series of protein bands in the TDW extract, four major protein bands in the AA extract and two protein bands in the TFA extract. Tandem mass spectrometry analysis of distinct protein bands identified potential innate immune factorshistone H2A, histone H3, histone H4, haemoglobin, cofilin and nucleoside diphosphate kinase in the TDW extract, and ubiquitin and histone H2B isoforms in acidic extracts of skin mucus of C. mrigala. The presence of these innate immune molecules suggests that skin mucus play an important role in the protection of the fish against microbial invasion.

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Section: Discussionmentioning

confidence: 99%

Evaluation of antibacterial activity and innate immune components in skin mucus of Indian major carp,Cirrhinus mrigala

et al. 2015

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“…Interestingly, comparative proteomic analysis shown that GAPDH was associated with the high susceptibility of channel catfish ( Ictalurus punctatus ) after immersion exposure to Edwardsiella ictaluri (Booth and Bilodeau‐Bourgeois, ), and GAPDH was also identified as up‐regulated protein in the buccal cavity mucus of parental tilapia ( Oreochromis spp.) (Iq and Shu‐Chien, ). Therefore, GAPDHs may have activities involved in the immune function or cellular stress responses in fish (Booth and Bilodeau‐Bourgeois, ; Iq and Shu‐Chien, ).…”

Section: Discussionmentioning

confidence: 99%

“…(Iq and Shu‐Chien, ). Therefore, GAPDHs may have activities involved in the immune function or cellular stress responses in fish (Booth and Bilodeau‐Bourgeois, ; Iq and Shu‐Chien, ). The mRNA expression of GAPDH2 was significantly up‐regulated in the liver of rockbream ( Oplegnathus fasciatus ) against the bacterial infections, which suggests that fish GAPDH2 may also be potentially involved in immune responses (Cho et al., ).…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of differential protein expression in the skin of zebrafish [Danio rerio (Hamilton, 1822)] infected with Aeromonas hydrophila

Lü

Wang

et al. 2013

J. Appl. Ichthyol.

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Summary Skin is an important component of the mucosal immune system in fish, however its molecular mechanism at the proteome‐level remains unclear. In this study, differentially expressed proteins in the skin of zebrafish Danio rerio responding to Aeromonas hydrophila infection were identified using two‐dimensional gel electrophoresis (2‐DE) coupled with mass spectrometry (MS) analysis. The results showed that 17 proteins were differentially expressed, six proteins of which were up‐regulated and 11 proteins down‐regulated. MHC class II DR α 1 domain, immunoglobulin heavy chain V‐region, glyceraldehyde‐3‐phosphate dehydrogenase, transcription factor AP‐2‐α, and actin were considered immune‐related molecules in the skin of zebrafish. Expression changes of 17 proteins were further determined by zebrafish cDNA microarray analysis. This is the first known report on proteome of fish skin against A. hydrophila, which will contribute to the understanding of the mucosal immune response, bacterial disease control and prevention.

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“…Parents of the caecilian amphibian Boulengerula taitanu modify their skin, which is eaten by their offspring using specialised teeth (Kupfer et al, 2006), and it has been proposed that the fry of mouth-brooding tilapia, e.g. Oreochromis spp., ingest mucus containing nutrients from the buccal cavity of their parents (Kishida and Specker, 2000;Iq and Shu-Chien, 2011). Other non-mammalian examples exist where animals are fed a nutritive secretion pre-parturition.…”

Section: Introductionmentioning

confidence: 99%

A toxic diet: transfer of contaminants to offspring through a parental care mechanism

Maunder

Buckley

Val

et al. 2013

Journal of Experimental Biology

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SUMMARYThe transfer of maternal contaminants to offspring during oogenesis and gestation is documented in many animals, and in mammals, contaminants may pass from mother to offspring during lactation. Although other non-mammalian vertebrates provide parental care in the form of nutritive secretions for offspring to feed from, the potential for toxicant transfer during nonmammalian parental care is rarely considered. The discus fish, Symphysodon spp., employs an unusual parental care strategy where fry feed on parental epidermal mucus for several weeks after hatching. This strategy has the potential to act as a method of contaminant transfer. In discus adults, both waterborne and dietary toxicants are sequestered and secreted into their epidermal mucus, the food on which fry depend. To determine whether parents could channel these contaminants directly to offspring, we exposed parents to aqueous cadmium (Cd) and recorded the subsequent feeding behaviour and Cd content of fry. Fry continued to feed normally from contaminated mucus and accumulated significant tissue concentrations of Cd. In conclusion, this parental care mechanism of the discus fish can expose offspring to harmful contaminants during the sensitive early stages of life and highlights that parent to offspring contaminant transfer after birth may be more widespread than previously thought.

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Proteomics of Buccal Cavity Mucus in Female Tilapia Fish (Oreochromis spp.): A Comparison between Parental and Non-Parental Fish

Cited by 33 publications

References 55 publications

Evaluation of antibacterial activity and innate immune components in skin mucus of Indian major carp,Cirrhinus mrigala

Evaluation of antibacterial activity and innate immune components in skin mucus of Indian major carp,Cirrhinus mrigala

Proteomic analysis of differential protein expression in the skin of zebrafish [Danio rerio (Hamilton, 1822)] infected with Aeromonas hydrophila

A toxic diet: transfer of contaminants to offspring through a parental care mechanism

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