Proteomic analysis of differential protein expression in the skin of zebrafish [<i>Danio rerio</i>
 (Hamilton, 1822)] infected with <i>Aeromonas hydrophila</i>

Lü, Aijun; Hu, Xiucai; Wang, Y.; Ming, Qing-Lei; Zhu, Aiguo; Shen, Lulu; Zhao-jun, Feng

doi:10.1111/jai.12318

Cited by 10 publications

(4 citation statements)

References 40 publications

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“…Several high-throughput protein assays have been conducted in various fish to determine the molecular mechanism of fish skin as a pathogen barrier at the proteome-level. A previous study identified 17 proteins differentially expressed in zebrafish ( Danio rerio ) skin under Aeromonas hydrophila infection, and these included the DR α 1 domain of the MHC class II and the immunoglobulin heavy chain V-region ( 21 ). Another study on the differential proteomics of the zebrafish skin in response to SVCV infection examined the by isobaric tags for relative and absolute quantitation (iTRAQ).…”

Section: Discussionmentioning

confidence: 99%

Differentially expressed proteins and microbial communities of the skin regulate disease resistance to Chinese tongue sole (Cynoglossus semilaevis)

Wang,

Tian,

Chen

2024

Front. Immunol.

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Vibriosis, caused by Vibrio, seriously affects the health of fish, shellfish, and shrimps, causing large economic losses. Teleosts are represent the first bony vertebrates with both innate and adaptive immune responses against pathogens. Aquatic animals encounter hydraulic pressure and more pathogens, compared to terrestrial animals. The skin is the first line of defense in fish, constituting the skin-associated lymphoid tissue (SALT), which belongs to the main mucosa-associated lymphoid tissues (MALT). However, little is known about the function of immunity related proteins in fish. Therefore, this study used iTRAQ (isobaric tags for relative and absolute quantitation) to compare the skin proteome between the resistant and susceptible families of Cynoglossus semilaevis. The protein integrin beta-2, the alpha-enolase isoform X1, subunit B of V-type proton ATPase, eukaryotic translation initiation factor 6, and ubiquitin-like protein ISG15, were highly expressed in the resistant family. The 16S sequencing of the skin tissues of the resistant and susceptible families showed significant differences in the microbial communities of the two families. The protein-microbial interaction identified ten proteins associated with skin microbes, including immunoglobulin heavy chain gene (IGH), B-cell lymphoma/leukemia 10 (BCL10) and pre-B-cell leukemia transcription factor 1 isoform X2 (PBX2). This study highlights the interaction between skin proteins and the microbial compositions of C. semilaevis and provides new insights into understanding aquaculture breeding research.

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Section: Discussionmentioning

confidence: 99%

Differentially expressed proteins and microbial communities of the skin regulate disease resistance to Chinese tongue sole (Cynoglossus semilaevis)

Wang,

Tian,

Chen

2024

Front. Immunol.

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“…In addition, the miRNAs interacting with the target genes and proteins were not only associated with immune response ( 7 , 45 ) but also played important roles in regulating the regulators of the immune network ( 28 , 58 ). In M. miiuy infected with Vibrio harveyi , miR-148 targeted and negatively regulated the expression of the MyD88 gene, and overexpression of miR-148 inhibited the inflammatory cytokine production of both IL-6 and IL-1β ( 16 ).…”

Section: Discussionmentioning

confidence: 99%

Multiomics analysis revealed miRNAs as potential regulators of the immune response in Carassius auratus gills to Aeromonas hydrophila infection

Huo

Bai

et al. 2023

Front. Immunol.

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The gill of fish is an important immune organ for pathogen defense, but its microRNA (miRNA) expression and regulatory mechanism remain unclear. In this study, we report on the histopathological and immunohistochemical features of the gills of the crucian carp Carassius auratus challenged with Aeromonas hydrophila. Small RNA libraries of the gills were constructed and sequenced on the Illumina HiSeq 2000 platform. A total of 1,165 differentially expressed miRNAs (DEMs) were identified in gills, of which 539 known and 7 unknown DEMs were significantly screened (p < 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the potential target genes/proteins were primarily involved in 33 immune-related pathways, in which the inflammatory responses were focused on the Toll-like receptor (TLR), mitogen-activated protein kinase (MAPK), and nuclear factor kappa B (NF-κB) signaling pathways. Moreover, the expression levels of 14 key miRNAs (e.g., miR-10, miR-17, miR-26a, miR-144, miR-145, and miR-146a) and their target genes (e.g., TNFα, TLR4, NF-κB, TAB1, PI3K, and IRAK1) were verified. In addition, the protein levels based on isobaric tags for relative and absolute quantification (iTRAQ) were significantly associated with the results of the quantitative real-time PCR (qRT-PCR) analysis (p < 0.01). miR-17/pre-miR-17 were identified in the regulation expression of the NF-κB target gene, and the phylogenetic tree analysis showed that the pre-miR-17 of C. auratus with the closest similarity to the zebrafish Danio rerio is highly conserved in teleosts. This is the first report of the multi-omics analysis of the miRNAs and proteins in the gills of C. auratus infected with A. hydrophila, thus enriching knowledge on the regulation mechanism of the local immune response in Cyprinidae fish.

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“…The up-regulation was similar to other fish-virus and bacterial studies [ 123 , 124 ], highlighting it as a therapeutic target for virus or bacteria-associated diseases. Furthermore, the proteomics approach of zebrafish skin infected with Aeromonas hydrophila revealed the increased expression of actin, myosin heavy chain, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [ 125 ]. The expression of both actin and myosin in the skin indicated that these proteins are important for the skin response of zebrafish to A. hydrophila infection.…”

Section: Multi-omics Perspective On Infectious Fish Diseases Studiesmentioning

confidence: 99%

“…It was suggested that the increased disease resistance in fish might be associated with the low expression of myosin. A low expression of myosin was proposed to inhibit muscle growth, which inhibits further pathogen infection [ 125 ]. Meanwhile, GAPDH was reported to have multiple roles in immune function, defense responses, antioxidant, and energy metabolism in fish [ 131 ].…”

Section: Multi-omics Perspective On Infectious Fish Diseases Studiesmentioning

confidence: 99%

Omics Strategies in Current Advancements of Infectious Fish Disease Management

Natnan

Mayalvanan

Jazamuddin

et al. 2021

Biology

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Aquaculture is an important industry globally as it remains one of the significant alternatives of animal protein source supplies for humankind. Yet, the progression of this industry is being dampened by the increasing rate of fish mortality, mainly the outbreak of infectious diseases. Consequently, the regress in aquaculture ultimately results in the economy of multiple countries being affected due to the decline of product yields and marketability. By 2025, aquaculture is expected to contribute approximately 57% of fish consumption worldwide. Without a strategic approach to curb infectious diseases, the increasing demands of the aquaculture industry may not be sustainable and hence contributing to the over-fishing of wild fish. Recently, a new holistic approach that utilizes multi-omics platforms including transcriptomics, proteomics, and metabolomics is unraveling the intricate molecular mechanisms of host-pathogen interaction. This approach aims to provide a better understanding of how to improve the resistance of host species. However, no comprehensive review has been published on multi-omics strategies in deciphering fish disease etiology and molecular regulation. Most publications have only covered particular omics and no constructive reviews on various omics findings across fish species, particularly on their immune systems, have been described elsewhere. Our previous publication reviewed the integration of omics application for understanding the mechanism of fish immune response due to microbial infection. Hence, this review provides a thorough compilation of current advancements in omics strategies for fish disease management in the aquaculture industry. The discovery of biomarkers in various fish diseases and their potential advancement to complement the recent progress in combatting fish disease is also discussed in this review.

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Proteomic analysis of differential protein expression in the skin of zebrafish [Danio rerio (Hamilton, 1822)] infected with Aeromonas hydrophila

Cited by 10 publications

References 40 publications

Differentially expressed proteins and microbial communities of the skin regulate disease resistance to Chinese tongue sole (Cynoglossus semilaevis)

Differentially expressed proteins and microbial communities of the skin regulate disease resistance to Chinese tongue sole (Cynoglossus semilaevis)

Multiomics analysis revealed miRNAs as potential regulators of the immune response in Carassius auratus gills to Aeromonas hydrophila infection

Omics Strategies in Current Advancements of Infectious Fish Disease Management

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