Proteomics in fisheries and aquaculture: An approach for food security

Nissa, Mehar Un; Pinto, Nevil; Parkar, Haifa; Goswami, Mukunda; Srivastava, Sanjeeva

doi:10.1016/j.foodcont.2021.108125

Cited by 33 publications

(19 citation statements)

References 112 publications

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“…Values with different superscripts in the same column are significantly different (p < 0.05). 1 Lysozyme activity (U mL −1 ). 2 Acid phosphatase (U mL −1 ).…”

Section: Innate Immune Responsementioning

confidence: 99%

“…Aquaculture is one of the most promising sources of animal protein [1]. However, in recent years, due to indiscriminate intensive aquaculture, water quality deterioration and diseases (viruses and bacteria) have become a serious threat to the aquaculture industry [2].…”

Section: Introductionmentioning

confidence: 99%

“…Whole-body proximate compositions of the Pacific white shrimp cultured in the control, SMG-A, and SMG-B groups after 6 weeks of culture1 . Values are means from triplicate groups of shrimps where the values in each row with different superscripts are significantly different (p < 0.05) 2.…”

mentioning

confidence: 99%

“…Whole-body amino acid compositions (mg 100 mg −1 ) of the Pacific white shrimp cultured in the control, SMG-A, and SMG-B groups after 6 weeks of culture1 . Values are mean of triplicate groups and presented as mean ± SD.…”

mentioning

confidence: 99%

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Evaluation of Salipiger thiooxidans and Exiguobacterium aestuarii from the Saemangeum Reservoir as Potential Probiotics for Pacific White Shrimp (Litopenaeus vannamei)

et al. 2022

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In this study, two bacterial species, Salipiger thiooxidans and Exiguobacterium aestuarii, were extracted and screened from the Saemangeum Reservoir. This study examined these species’ suitability as a probiotic by confirming the effects of S. thiooxidans and E. aestuarii added to rearing water for L. vannamei. Three experimental groups were evaluated for 6 weeks: (1) a control group reared in natural (i.e., untreated) water (CON), (2) an experimental group in which S. thiooxidans was added to natural water (SMG-A), and (3) natural water inoculated with E. aestuarii (SMG-B). The SMG-B group inoculated with E. aestuarii showed significantly higher final body weight, weight gain, specific growth rates, and feed efficiency than the control group. The SMG-B group inoculated with E. aestuarii exhibited significantly higher levels of serum lysozyme, and ACP and ALP activity than the control and SMG-A groups. The SMG-A and SMG-B groups inoculated with probiotics showed significantly lower total ammonia nitrogen and nitrite than the control group. Our findings suggest that S. thiooxidans and E. aestuarii extracted from the Saemangeum Reservoir can improve the water quality of aquaculture water, and, in particular, E. aestuarii is a potential probiotic for L. vannamei.

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“…Values with different superscripts in the same column are significantly different (p < 0.05). 1 Lysozyme activity (U mL −1 ). 2 Acid phosphatase (U mL −1 ).…”

Section: Innate Immune Responsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of Salipiger thiooxidans and Exiguobacterium aestuarii from the Saemangeum Reservoir as Potential Probiotics for Pacific White Shrimp (Litopenaeus vannamei)

et al. 2022

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“…Some key challenges faced by aquaculture like health, nutrition, sustainable quality fish production, safety, and environmental issues can be addressed by genomic and proteomic studies. 4 Mass spectrometry-based proteomics has achieved remarkable progress over the years owing to rapid advancements in mass spectrometry in addition to the evolution of spectral analysis algorithms. Both the gel-based and non-gel-based proteomic approaches have been used to study fish physiology, quality, nutrition, disease, and ecotoxicology.…”

Section: ■ Introductionmentioning

confidence: 99%

Organ-Based Proteome and Post-Translational Modification Profiling of a Widely Cultivated Tropical Water Fish, Labeo rohita

et al. 2021

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Proteomics has enormous applications in human and animal research. However, proteomic studies in fisheries science are quite scanty particularly for economically important species. Few proteomic studies have been carried out in model fish species, but comprehensive proteomics of aquaculture species are still scarce. This study aimed to perform a comprehensive organ-based protein profiling of important tissue samples for one of the most important aquaculture species,Labeo rohita.Deep proteomic profiling of 17 histologically normal tissues, blood plasma, and embryo provided mass-spectrometric evidence for 8498 proteins at 1% false discovery rate that make up about 26% of the total annotated protein-coding sequences in Rohu. Tissue-wise expression analysis was performed, and the presence of several biologically important proteins was also verified using a targeted proteomic approach. We identified the global post-translational modifications (PTMs) in terms of acetylation (N-terminus and lysine), methylation (N-terminus, lysine, and arginine), and phosphorylation (serine, threonine, and tyrosine) to present a comprehensive proteome resource. An interactive web-based portal has been developed for an overall landscape of protein expression across the studied tissues of Labeo rohita (www.fishprot.org). This draft proteome map of Labeo rohita would advance basic and applied research in aquaculture to meet the most critical challenge of providing food and nutritional security to an increasing world population.

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RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis

Rusu

Broadbent

Wade

et al. 2022

Aquaculture Fish & Fisheries

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During sample collection and transport, high‐quality nucleic acids, proteins and metabolites are preserved by snap‐freezing (SF) samples in liquid nitrogen or dry ice. In remote aquaculture facilities, SF materials are not readily available and can be hazardous during sampling and transport. RNAlater is widely used in aquaculture for transcriptome studies, but its effect on proteome stability needs further investigation. Here, we used proteomics to demonstrate that RNAlater (RL) preserved liver samples similarly to SF samples. Additionally, 69 proteins (∼2.6%) comprising ribosomal proteins, transcription cofactors, translational factors, proteases and transmembrane proteins were significantly less abundant in the RL proteome. These proteins are expected to be denatured by RL and are cellular components of ribosomes and nucleosomes and involved in proteolysis, transcription and translation. We also demonstrated that RL did not influence abundance of important proteins originally identified in liver samples from salmon reared under heat stress, hypoxia or specific feeding regimes. These findings suggest that preserving samples in RL is suitable for proteomics and offer a safer and effective preservation technology for use in remote aquaculture locations.

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Proteomics in fisheries and aquaculture: An approach for food security

Cited by 33 publications

References 112 publications

Evaluation of Salipiger thiooxidans and Exiguobacterium aestuarii from the Saemangeum Reservoir as Potential Probiotics for Pacific White Shrimp (Litopenaeus vannamei)

Evaluation of Salipiger thiooxidans and Exiguobacterium aestuarii from the Saemangeum Reservoir as Potential Probiotics for Pacific White Shrimp (Litopenaeus vannamei)

Organ-Based Proteome and Post-Translational Modification Profiling of a Widely Cultivated Tropical Water Fish, Labeo rohita

RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis

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