Proteomics identifies a novel role of fibrinogen-like protein 1 in Crohn’s disease

Sun, Xuemei; Qiao, Lixin; Gong, Jing; Wen, Kai; Xu, Zhizhong; Yang, Bo‐Lin

doi:10.3748/wjg.v27.i35.5946

Cited by 9 publications

(12 citation statements)

References 23 publications

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“…In a report on Crohn’s disease and FGL1, intestinal tissues, which involve local immunity, were examined. 21 All of these findings suggest a fundamental role of FGL1 in regulating immune-mediated inflammation.…”

Section: Discussionmentioning

confidence: 98%

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Fibrinogen-Like Protein 1 as a Novel Biomarker of Psoriasis Severity

Sun¹,

Liu²,

Chen³

et al. 2022

JIR

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Background Psoriasis is an immune-mediated chronic systemic inflammatory skin disease whose diagnosis and severity assessment pose challenges for clinicians worldwide. The use of serum biomarkers facilitates the early diagnosis and treatment of psoriasis. Methods This case–control study compared tumor necrosis factor α (TNF-α), interleukin (IL)-6, IL-17, IL-10, and fibrinogen-like protein 1 (FGL1) levels of 139 untreated psoriasis patients and 140 healthy controls. Serum samples were collected, and enzyme-linked immunosorbent assays were performed to quantify their levels. Subgroups were analyzed according to abnormal lipid metabolism status. Results Compared to controls, patients with psoriasis exhibited lower concentrations of serum TNF-α, IL-17, and FGL1 ( P < 0.05). A correlation analysis showed that FGL1 was inversely correlated with high-density lipoprotein cholesterol and IL-17 in the psoriatic state. Stepwise multiple regression analysis revealed that FGL1 and total cholesterol were the independent determinants of Psoriasis Area and Severity Index (PASI) score in psoriasis patients. The area under the receiver operating characteristic curve of FGL1 assessing moderate-to-severe psoriasis and mild psoriasis was 0.70, while the area under the curve (AUC) assessing severe psoriasis and mild-to-moderate psoriasis was 0.67, better than that of IL-17. In addition, FGL1, but not IL-17, was able to identify psoriasis with abnormal lipid metabolism to a certain extent (AUC = 0.60). Conclusion In conclusion, serum FGL1 may be a promising biomarker for diagnosing and staging psoriasis. It may also be involved in its progression and comorbid abnormal lipid metabolism.

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Section: Discussionmentioning

confidence: 98%

“… 19 Studies have shown that FGL1 may be a new biomarker for immune-related diseases including Crohn’s disease and rheumatoid arthritis. 20 , 21 Altogether, these findings suggest that FGL1 is associated with psoriasis pathogenesis and may be useful for diagnosing and staging its severity.…”

Section: Introductionmentioning

confidence: 85%

Fibrinogen-Like Protein 1 as a Novel Biomarker of Psoriasis Severity

Sun¹,

Liu²,

Chen³

et al. 2022

JIR

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“…Additionally, the role of FGL1 in CD onset and development is unknown. Our previous study has found increased FGL1 levels in both intestinal mucosa and blood plasma of CD patients (Sun et al, 2021). Overexpression of FGL1 enhances lipopolysaccharide-induced inflammatory response in IECs by activating NF-κB signaling (Sun et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Our previous study has screened out fibrinogen-like protein 1 (FGL1) as a potential biomarker and therapeutic target of CD by proteomics ( Sun et al, 2021 ). FGL1, also known as hepassocin or hepatocyte-derived fibrinogen-related protein 1 (HFREP1), is a hepatocyte-secreted acute phase reactant involving in a variety of inflammatory diseases ( Liu and Ukomadu, 2008 ; Liu et al, 2020 ; Sun et al, 2021 ; Liu et al, 2022 ; Sui et al, 2022 ). Functionally, FGL1 belongs to a fibrinogen superfamily, and participates in the formation of a plasma clot through non-covalently binding to fibrin and fibrinogen ( Rijken et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Xue-Jie-San restricts ferroptosis in Crohn’s disease via inhibiting FGL1/NF-κB/STAT3 positive feedback loop

Gao

Zhang

et al. 2023

Front. Pharmacol.

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Crohn’s disease (CD) is an incurable inflammatory bowel disease due to unclear etiology and pathogenesis. Accumulating evidences have shown the harmful role of ferroptosis in CD onset and development. Additionally, fibrinogen-like protein 1 (FGL1) has been verified to be a potential therapeutic target of CD. Xue-Jie-San (XJS) is an effective prescription for treating CD. However, its therapeutic mechanism has not been fully elucidated. This study aimed to determine whether XJS alleviating CD via regulating ferroptosis and FGL1 expression. A colitis rat model was induced by 2,4,6-trinitrobenzene sulfonic acid and treated with XJS. The disease activity indices of the colitis rats were scored. Histopathological damage was assessed using HE staining. ELISA was performed to examine inflammatory cytokines. Transmission electron microscopy was utilized to observe ultrastructure changes in intestinal epithelial cells (IECs). Iron load was evaluated by examining iron concentrations, the expressions of FPN, FTH and FTL. Lipid peroxidation was investigated through detecting the levels of ROS, 4-HNE, MDA and PTGS2. Furthermore, the SLC7A11/GSH/GPX4 antioxidant system and FGL1/NF-κB/STAT3 signaling pathway were examined. The results showed that colitis was dramatically ameliorated in the XJS-treated rats as evidenced by relief of clinical symptoms and histopathological damages, downregulation of pro-inflammatory cytokines IL-6, IL-17 and TNF-α, and upregulation of anti-inflammatory cytokine IL-10. Furthermore, XJS administration led to ferroptosis inhibition in IECs by reducing iron overload and lipid peroxidation. Mechanistically, XJS enhanced the SLC7A11/GSH/GPX4 antioxidant system negatively regulated by the FGL1/NF-κB/STAT3 positive feedback loop. In conclusion, XJS might restrain ferroptosis in IECs to ameliorate experimental colitis by inhibition of FGL1/NF-κB/STAT3 positive feedback loop.

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“…FGL1 is a member of the fibrinogen family and is critical for the formation of fibrin clots. FGL1 is highly expressed during intestinal inflammation and is hypothesised to promote the expression of pro-inflammatory cytokines through the activation of the NF-κB signalling pathway (X. L. Sun et al, 2021). For example, FGL1 is highly expressed in rheumatoid arthritis (Liu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Analysis of differentially expressed microRNAs in bovine mammary epithelial cells treated with lipoteichoic acid

Shen

Yan

et al. 2022

Animal Physiology Nutrition

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Mastitis is one of the most common diseases of dairy cattle and can be caused by physical stress, chemicals and microbial infection. Staphylococcus aureus is the most common pathogens that induce mastitis in dairy cattle. In this study, bovine mammary epithelial cells (BMECs) were treated either with lipoteichoic acid (LTA, 30 µg/ml) or 1 × phosphate‐buffer saline (PBS, control) and RNA‐Seq was applied to explore the effect of LTA on the expression microRNAs (miRNAs) in BMECs. Compared to the control group, 43 miRNAs were significantly up‐regulated and eight miRNAs were significantly down‐regulated. Additionally, 724 genes were significantly up‐regulated and 13 genes were significantly down‐regulated in LTA group relative to the control group. Bta‐miR‐196a, bta‐miR‐2285aj‐5p, bta‐miR‐143, bta‐miR‐2433, bta‐miR‐2284f and bta‐miR‐2368‐3p were selected from 51 differentially expressed miRNAs and are discussed in this manuscript. Target gene prediction revealed that the target genes of these six miRNAs were all differentially expressed, including MT1E, SPDYA, FGL1, TLR2, PAPOLG, ZDHHC17 and SMC4. Subsequently, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the target genes with differentially expressed miRNAs were enriched in mitogen‐activated protein kinase (MAPK) signalling pathway, rheumatoid arthritis and cancer. Therefore, the results of this study provided new evidences for the molecular mechanism of LTA‐induced mastitis, which may provide new targets for the diagnosis and treatment of mastitis in dairy cattle.

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Proteomics identifies a novel role of fibrinogen-like protein 1 in Crohn’s disease

Cited by 9 publications

References 23 publications

Fibrinogen-Like Protein 1 as a Novel Biomarker of Psoriasis Severity

Fibrinogen-Like Protein 1 as a Novel Biomarker of Psoriasis Severity

Xue-Jie-San restricts ferroptosis in Crohn’s disease via inhibiting FGL1/NF-κB/STAT3 positive feedback loop

Analysis of differentially expressed microRNAs in bovine mammary epithelial cells treated with lipoteichoic acid

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