Proteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approach

Abstract

BackgroundInduced resistance (IR) can be part of a sustainable plant protection strategy against important plant diseases. β-aminobutyric acid (BABA) can induce resistance in a wide range of plants against several types of pathogens, including potato infected with Phytophthora infestans. However, the molecular mechanisms behind this are unclear and seem to be dependent on the system studied. To elucidate the defence responses activated by BABA in potato, a genome-wide transcript microarray analysis in combination with label-free quantitative proteomics analysis of the apoplast secretome were performed two days after treatment of the leaf canopy with BABA at two concentrations, 1 and 10 mM.ResultsOver 5000 transcripts were differentially expressed and over 90 secretome proteins changed in abundance indicating a massive activation of defence mechanisms with 10 mM BABA, the concentration effective against late blight disease. To aid analysis, we present a more comprehensive functional annotation of the microarray probes and gene models by retrieving information from orthologous gene families across 26 sequenced plant genomes. The new annotation provided GO terms to 8616 previously un-annotated probes.ConclusionsBABA at 10 mM affected several processes related to plant hormones and amino acid metabolism. A major accumulation of PR proteins was also evident, and in the mevalonate pathway, genes involved in sterol biosynthesis were down-regulated, whereas several enzymes involved in the sesquiterpene phytoalexin biosynthesis were up-regulated. Interestingly, abscisic acid (ABA) responsive genes were not as clearly regulated by BABA in potato as previously reported in Arabidopsis. Together these findings provide candidates and markers for improved resistance in potato, one of the most important crops in the world.

Plant Resistance Inducers against Pathogens in Solanaceae Species—From Molecular Mechanisms to Field Application

“…The general notion is that priming has a low fitness cost and that only subtle changes take place when induction occurs by a PRI. However, at least the latter can be questioned since there are now several genome-wide studies showing that substantial changes in the expression of genes, proteins and metabolites take place (for example [11,12]). Based on “-omics” studies, it was recently suggested to divide the priming phenomenon into three different stages: a “priming phase”, a “post-challenge primed state” and a “transgenerational primed state” [13].…”

Functional Analogues of Salicylic Acid and Their Use in Crop Protection

“…BABA also provided significant control of potato late blight in the field when used alone or in combination of the standard fungicide [51]. In potato, it was able to induce HR-like lesions surrounded by callose and the production of H 2 O 2 , as well as the enhancement of phenolic content and activation of PR1 [52]. To elucidate in depth molecular mechanisms of BABA-induced resistance against potato late blight, Bengtsson et al, developed an original approach based on a transcript analysis in combination with quantitative proteomic analysis of the apoplast secretome.…”

“…Arabidopsis/hyaloperonospora parasitica, Botrytis cinerea (Laboratory) [46] Brassica napus/Leptosphaeria maculans (Laboratory) [49] Lettuce/Bremia lactucae (Laboratory) [47] Tomato/Phytophthora infestans (Laboratory) [48] Potato/Phytophthora infestans (Laboratory/Field) [51,52] Grapevine/Plasmopara viticola [50] …”

Cell Wall Proteomics as a Means to Identify Target Genes to Improve Second‐Generation Biofuel Production

“…CWPs are essential to the wall functions such as modiication of the cell wall components, its structure, signalling, interaction with the plasma membrane and response to stresses [27]. Several factors can modify the cell wall proteome content, such as development [28 31] and biotic or abiotic stresses [32,33].…”

“…CWPs are essential to the wall functions such as modiication of the cell wall components, its structure, signalling, interaction with the plasma membrane and response to stresses [27]. Several factors can modify the cell wall proteome content, such as development [28 31] and biotic or abiotic stresses [32,33].CWPs share three common characteristics: a signal peptide to be targeted to the secretory pathway, no intracellular retention motif and the absence of hydrophobic trans-membrane domains. The signal peptide presents a positive charge at its N-terminus, a hydrophobic central region and a polar C-terminus [34].…”