Proteomics analysis suggests broad functional changes in potato leaves triggered by phosphites and a complex indirect mode of action against Phytophthora infestans

Lim, Sang-Hyun; Borza, Tudor; Peters, R. D.; Coffin, R. H.; Al-Mughrabi, Khalil I.; Pinto, Devanand M.; Wang‐Pruski, Gefu

doi:10.1016/j.jprot.2013.03.010

Cited by 88 publications

(69 citation statements)

References 56 publications

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“…We found in this study that Phi induced changes in levels of alanine and the abundance of valine, glutamine and asparagine were reduced in the Phi-treated plants. This result is consistent with the report by Lim et al [9]. Many studies have also demonstrated a role of amino acids in the establishment of resistance against plant pathogens [46] [48] [53], and that the alteration of specific metabolite levels by feeding or genetic manipulation of plant metabolic pathways can lead to resistance against pathogens.…”

Section: Amino Acid Changes From Different Treatment Groupssupporting

confidence: 92%

“…The amino acids isoleucine, leucine and valine are important in the production of plant defense secondary metabolites such as cyanogenic glycosides, and glucosinolates [52]. Lim et al [9] examined the direct effect of Phi application on potato leaves using comparative proteomics and identified significant changes in quantity of proteins after Phi treatment in potato leaves. They reported that down-regulated proteins were involved in metabolism and energy functions, such as amino acid metabolism, starch metabolism, photosynthesis, glycolysis and tricarboxylic acid (TCA) cycle; whereas majority up-regulated proteins were defense-related and associated with the salicylic acid (SA) dependent pathway, antimicrobial activity, the ROS pathway, the Ca 2+ dependent pathway and the hypersensitivity reaction (HR).…”

Section: Amino Acid Changes From Different Treatment Groupsmentioning

confidence: 99%

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Metabolomics Profile of Potato Tubers after Phosphite Treatment

Gao¹,

Locke²,

Zhang³

et al. 2018

AJPS

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Phosphite (Phi)-based fungicides are used to control the oomycete Phytophthora infestans which causes late blight disease, the most devastating disease in potatoes. In order to examine the effects of Phi-based fungicides on potato tubers through foliar or post-harvest application, a metabolite profiling approach based on gas chromatography coupled to mass spectrometry (GC-MS) has been established. A total of 132 metabolites were detected using the GC-MS approach. Among these, 34 metabolites were identified after normalization and annotated with a compound name with standard mass spectral library. Metabolomic analysis of Phi-treated plants showed significant differences in the levels of many metabolites especially amino acids. Multivariate statistical approaches, such as principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), were employed to explore the relationships between metabolites to detect group differences. A good discrimination between the control and the Phi-treated plants was observed, which demonstrated that significant changes in the metabolite profile have been caused by the two different Phi applications (foliar or post-harvest). This finding suggests that the alteration of specific metabolite levels by accumulation of Phi can lead to resistance against the pathogen.

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Section: Amino Acid Changes From Different Treatment Groupssupporting

confidence: 92%

Section: Amino Acid Changes From Different Treatment Groupsmentioning

confidence: 99%

Metabolomics Profile of Potato Tubers after Phosphite Treatment

Gao¹,

Locke²,

Zhang³

et al. 2018

AJPS

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“…The tubers harvested at the end of the growing season were used for tuber rot evaluation. Detached leaf infection assay [22] was performed for all 100 individuals using P. infestans mating type A2, genotype US-8. Each leaf was placed in a Petri dish (15 cm in diameter) and inoculated with approximately 50,000 sporangia, applied on both sides of the leaf.…”

Section: Detached Leaf Infection Assay and Tuber Infection Assaymentioning

confidence: 99%

DNA Markers for Selection of Late Blight Resistant Potato Breeding Lines

Chen¹,

Borza²,

Byun³

et al. 2017

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Potato late blight, caused by the oomycete Phytophthora infestans, is one of the most devastating diseases in the agricultural sector around the world. Many genes (R genes) conferring resistance to late blight have been identified in various potato species and most of these R genes have been used in potato breeding. The aim of this study was to develop and validate PCR-based assays for the R genes Rpi-blb1, Rpi-blb2, Rpi-blb3 and Rpi-bt1, to distinguish between late blight resistant and late blight susceptible potato progeny in the given breeding background. A total of 100 breeding progeny were screened for the presence of these R genes and tested for resistance against P. infestans mating type A2, genotype US-8 strain, using detached leaf and tuber rot assays. PCR products for the Rpi-blb1 and Rpi-bt1 resistance genes were identified in the resistant progeny but were absent in the susceptible ones; therefore these PCR assays could differentiate between late blight resistant and susceptible plants. Genotypic data from the DNA markers derived from the Rpi-blb1 and Rpi-bt1 genes was found to correlate with the phenotypic data for foliar late blight but not with data for tuber rot. Our results demonstrate that markers derived from these two R genes could be useful for marker-assisted selection (MAS) for foliar late blight resistance in potato breeding programs.

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“…Plantas tratadas com a molécula apresentaram os menores valores de queima observados, não diferindo apenas das plantas tratadas com o fungicida Carbendazim. Isso provavelmente se deu pelo complexo modo de ação dos fosfitos que, além de agir diretamente sobre os microrganismos, potencializa a resposta de defesa das plantas, induzindo a resistência destas (7,15).…”

Section: Resultsunclassified

Produtos naturais disponíveis comercialmente induzem o acúmulo de fitoalexinas em cotilédones de soja e mesocótilos de sorgo

et al. 2017

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RESUMO O objetivo deste trabalho foi determinar o potencial de diferentes doses do extrato da alga marinha Ascophyllum nodosum (AN), do fosfito de potássio (FP), do óleo de nim (ON) e do extrato pirolenhoso (EP), todos produtos naturais disponíveis comercialmente, em induzir o acúmulo de fitoalexinas em cotilédones de soja e mesocótilos de sorgo. Foram utilizadas 5 doses para cada produto, sendo: 0,0, 0,1, 0,2, 0,3 e 0,4 mL/L para o FP e 0,0, 0,1, 0,3, 0,5 e 1,0 mL/L para os demais. Além destes, o tratamento com o acibenzolar-S-metílico (ASM) serviu de controle positivo. A análise de variância demonstrou que todos os produtos testados foram eficientes em induzir fitoalexinas em cotilédones de soja e mesocótilos de sorgo, com destaque especial para o AN que, nas duas maiores doses, diferiu significativamente tanto da dose zero quanto do controle positivo. Além disso, o AN foi, numericamente, superior aos demais produtos. O aumento progressivo das doses resultou em aumentos também progressivos de fitoalexinas em soja e em sorgo, demonstrando alta correlação do ‘fator dose’ com a quantidade de fitoalexina produzida. Esses resultados apontam para a possibilidade de utilização desses produtos como componentes bastante promissores no manejo integrado de doenças de plantas.

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Proteomics analysis suggests broad functional changes in potato leaves triggered by phosphites and a complex indirect mode of action against Phytophthora infestans

Cited by 88 publications

References 56 publications

Metabolomics Profile of Potato Tubers after Phosphite Treatment

Metabolomics Profile of Potato Tubers after Phosphite Treatment

DNA Markers for Selection of Late Blight Resistant Potato Breeding Lines

Produtos naturais disponíveis comercialmente induzem o acúmulo de fitoalexinas em cotilédones de soja e mesocótilos de sorgo

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