DNA Markers for Selection of Late Blight Resistant Potato Breeding Lines

Chen, Shaohua; Borza, Tudor; Byun, B. H.; Coffin, R. H.; Coffin, Joyce; Peters, R. D.; Wang‐Pruski, Gefu

doi:10.4236/ajps.2017.86079

Cited by 19 publications

(13 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Presence of Rpi-blb1 late blight resistant gene was shown in lines 13, 41, 61, 72, 54 and 70. Similar findings were reported by Chen et al [16], who stated that genotypic and phenotypic data were related in case of late blight disease when DNA markers derived from the Rpi-blb1 gene were used for marker assisted selection. However, from these findings it is revealed that, late blight resistant gene had been successfully transferred to the recipient variety from the donor, in most of the selected LB resistant lines.…”

Section: Late Blight Gene Confirmationsupporting

confidence: 88%

See 1 more Smart Citation

Field Screening and Marker Assisted Selection of Late Blight Resistant Potato Lines

Islam¹,

Raihan²,

Nahiyan³

et al. 2018

IJPSS

View full text Add to dashboard Cite

Late blight (LB), caused by Phytophthora infestans (Mont.) de Bary, is the most destructive disease of potato. The main objective of this study was to evaluate 72 potato lines, derived from crosses of the recipient cv. ACI Pakri-1 (female) and a LB resistant donor variety (male), against LB disease. Parent materials, LB susceptible varieties Diamant and BARI Alu-40 and LB resistant variety BARI Alu-77 were used as check varieties. The experiment was conducted with three levels of inoculums pressure (i) LB inoculation & no fungicide, (ii) No LB inoculation & no fungicide and (iii) No LB inoculation & fungicide. LB infection was assessed at 10 day intervals by scoring the percentage of foliage destruction. Subsequently, the area under the disease progress curve (AUDPC), relative AUDPC and susceptible scale value were estimated. Three categories of potato lines were selected considering level of LB infection and tuber appearance -(a) LB resistant -8 lines, (b) LB tolerant -14 lines, and (c) LB susceptible -14 lines. The foliage destruction of selected LB resistant

show abstract

Section: Late Blight Gene Confirmationsupporting

confidence: 88%

“…DNA was isolated from sprout by following CTAB method [15]. PCR was performed with selected markers [16]. Amplified PCR products were detected on a 3% agarose gel stained in 1 x Tris-base EDTA buffer and visualised on a UV trans-illuminator.…”

Section: Lb Gene Confirmation Through Pcrmentioning

confidence: 99%

Field Screening and Marker Assisted Selection of Late Blight Resistant Potato Lines

Islam¹,

Raihan²,

Nahiyan³

et al. 2018

IJPSS

View full text Add to dashboard Cite

show abstract

“…Despite a wide range of genetic studies of the potato tubers eye depth trait and the identification of genes and QTL associated with eye depth variability, DNA markers are still not used in the breeding of potatoes to this trait. Meanwhile, the use of diagnostic DNA markers allows to carry out more efficient prebreeding research (screening of potato genetic resources to identify donors of valuable allelic variants) and markerassisted selection in breeding programs (Gebhardt et al, 2006;Chen et al, 2017;Klimenko et al, 2017Klimenko et al, , 2019. We were able to develop a number of new PCR markers that can be convenient for screening the genetic resources and breeding material of potato.…”

Section: Resultsmentioning

confidence: 99%

Genomic regions of Solanum tuberosum L. associated with the tuber eye depth

et al. 2020

View full text Add to dashboard Cite

Potato (Solanum tuberosum L.) is one of the most important food crops in the world. The genome of this potato species is autotetraploid and has a high level of heterozygosity, also this potato species is a cross-pollinated plant. These characteristics complicate the genetic analysis and breeding process. The tuber’s eye depth is an important trait that affects the suitability of potato varieties for processing. Potato breeding for this trait is based on phenotypic assessment. Identification of the loci that control tuber eye depth would allow diagnostic markers for the marker-assisted selection to be created. The aim of this study is to search for loci associated with the eye depth by analyzing Solanum tuberosum varieties from the GenAgro collection of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, genotyped using the Illumina 22K SNP potato array DNA chip. The 24 significant markers associated with the “eye depth” trait were identified using 15,214 SNP markers genotyped with the Illumina 22K SNP potato array chip and the general linear model (GLM) taking into account the population structure. Data obtained showed the presence of SNPs in four genomic regions: on chromosome 4 (1 marker in the 3.92 Mb area), 5 (1 marker in the 4.67 Mb area) and 10 (1 marker in the 4.87 Mb area and 21 markers in the region between 48.1–48.9 Mb). The results of localization in the region 48.1–48.9 Mb of chromosome 10 correspond to previously published studies, the remaining three regions were detected for the first time. DNA sections containing SNPs linked to the tuber’s eye depth were studied in the SolTub_3.0 potato genome assembly (https:// plants.ensembl.org/). KASP markers were developed based on the data obtained. It will be possible to screen the breeding material and to breed the varieties more effectively using current markers associated with a shallow tuber’s eye depth.

show abstract

“…The genes R1, R2 and its orthologue Rpi-abpt, R2-like, and Rpi-blb3; R3a and R3b, R8, R9a, the orthologues Rpi-blb1 and Rpi-sto1, Rpi-blb2, Rpi-vnt1 and Rpi-chc1 have been located on chromosomes 4 to 11 and characterized most comprehensively [11]. DNA markers corresponding to the fragments of Rpi genes introgressed into cultivated potato from wild relatives are actively deployed for screening breeding material to select promising genotypes combining several Rpi genes [12][13][14]. The dRenSeq method for diagnostic sequencing genome target fragments ensures a high degree of certainty of the identification of full-length sequences of known Rpi genes in genetic collections and breeding material [15].…”

Section: Introductionmentioning

confidence: 99%

Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars

et al. 2021

View full text Add to dashboard Cite

(1) Background: Although resistance to pathogens and pests has been researched in many potato cultivars and breeding lines with DNA markers, there is scarce evidence as to the efficiency of the marker-assisted selection (MAS) for these traits when applied at the early stages of breeding. A goal of this study was to estimate the potential of affordable DNA markers to track resistance genes that are effective against the pathogen Phytophthora infestans (Rpi genes), as a practical breeding tool on a progeny of 68 clones derived from a cross between the cultivar Sudarynya and the hybrid 13/11-09. (2) Methods: this population was studied for four years to elucidate the distribution of late blight (LB) resistance and other agronomical desirable or simple to phenotype traits such as tuber and flower pigmentation, yield capacity and structure. LB resistance was phenotypically evaluated following natural and artificial infection and the presence/absence of nine Rpi genes was assessed with 11 sequence-characterized amplified region (SCAR) markers. To validate this analysis, the profile of Rpi genes in the 13/11-09 parent was established using diagnostic resistance gene enrichment sequencing (dRenSeq) as a gold standard. (3) Results: at the early stages of a breeding program, when screening the segregation of F1 offspring, MAS can halve the workload and selected SCAR markers for Rpi genes provide useful tools.

show abstract

DNA Markers for Selection of Late Blight Resistant Potato Breeding Lines

Cited by 19 publications

References 24 publications

Field Screening and Marker Assisted Selection of Late Blight Resistant Potato Lines

Field Screening and Marker Assisted Selection of Late Blight Resistant Potato Lines

Genomic regions of Solanum tuberosum L. associated with the tuber eye depth

Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars

Contact Info

Product

Resources

About