Proteomics analysis of human cerebrospinal fluid

Yuan, Xianglin; Desiderio, Dominic M.

doi:10.1016/j.jchromb.2004.06.044

Cited by 108 publications

(96 citation statements)

References 45 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, in one of this work, the removal of high-abundant proteins by immunocapture and ultrafiltration allowed the detection by 2-D LC-MS/MS analysis of several new sets of unique CSF proteins. The study of the global CSF proteome was also completed by peptidomics investigations which revealed the presence in this fluid of many neuroendocrine peptides and neurosecretory proteins [20][21][22].…”

Section: Proteomics Analysis Of the Normal Csf Proteomementioning

confidence: 99%

Clinical proteomics of the cerebrospinal fluid: Towards the discovery of new biomarkers

Roche

Gabelle

Lehmann

2008

Proteomics Clinical Apps

View full text Add to dashboard Cite

The cerebrospinal fluid (CSF) circulates within the CNS where it plays an essential physiological role in homeostasis of neuronal cells. This biological fluid has an important protein diversity that results from both filtration of serum through the blood-brain barrier and production/secretion of neuronal peptides and proteins. Changes in CSF composition depend on blood proteome, CSF circulation alterations, as well as physiological or pathological brain status. Hence, CSF proteomic analysis gives a unique opportunity to detect and describe biomarkers in neurological affections. Although lumbar puncture is considered as invasive, post lumbar puncture events remain rare and minor. Nevertheless, CSF biological analysis is currently limited to a small number of parameters and clinical situations suggesting mainly meningitis, malignancies or dementia. Few CSF proteomic studies have been performed in comparison to those on blood. In this review, we will provide a proteomics description of the CSF, summarize the current clinical use of this fluid and describe its clinical proteomics examination.

show abstract

Section: Proteomics Analysis Of the Normal Csf Proteomementioning

confidence: 99%

Clinical proteomics of the cerebrospinal fluid: Towards the discovery of new biomarkers

Roche

Gabelle

Lehmann

2008

Proteomics Clinical Apps

View full text Add to dashboard Cite

show abstract

“…Among the albumin depletion columns, Cibacron Blue-Sepharose (73) media or monoclonal antibodies (74), (75), (76) are the most common affinity materials. The protein concentration is much higher in serum than in CSF and reducing the volume of CSF might be necessary before the affinity removal of albumin (77).…”

Section: Prefractionation Techniquesmentioning

confidence: 99%

“…CSF is secreted from several central nervous system (CNS) tissues; in particular, from the ventricular choroid plexus, and is directly connected to the extracellular (interstitial) fluid. The extracellular fluid surrounds the neurons and glia, therefore changes of protein and peptide concentration and modifications in CSF may reflect ongoing pathological processes in the CNS (77). The total volume of CSF in the human ventricular system is about 100-150 ml.…”

Section: Cerebrospinal Fluidmentioning

confidence: 99%

Prefractionation of Cerebrospinal Fluid to Enhance Glycoprotein Concentration Prior to Structural Determination with FT−ICR Mass Spectrometry

2005

View full text Add to dashboard Cite

När vi leva, låtom oss leva. While we live, let us live. "Let us enjoy life." Dum vivimus vivamus.3 Mass spectrometry for comparative proteomics of degenerative and regenerative processes in the brain ABSTRACT Biological processes involve changes at the protein level which can be detected and quantified. Proteomics aims to determine protein changes from a normal state, for instance to measure the degree of recovery in a biological system or the state of disease progression. Mass spectrometry is the most important tool in proteomics for the identification of proteins and determination of post-translational modifications such as glycosylation. Glycoproteins were found to be altered in patients with Alzheimer's disease (AD), which is the most common form of dementia. Changes in glycosylation levels were quantified with a glycoprotein-specific stain after gel separation. Glycan structures were determined with mass spectrometry in this thesis. Protein quantification with mass spectrometric methods is based on stable isotope labeling of proteins and labeled cell cultures can be used as internal standards for tissue proteomics. Quantitative proteomics was applied to assess protein expression levels with mass spectrometry in the murine brain after specific neurosurgery to study regenerative processes.In order to compare glycosylated proteins in cerebrospinal fluid (CSF) from individual AD patients with healthy control individuals, glycoproteomic methods were developed. To enhance the concentration of glycoproteins prior to gel separation, affinity chromatography of CSF was the most suitable prefractionation method for removing the most abundant CSF protein albumin. CSF proteins were separated with narrow pH-range two-dimensional gel electrophoresis followed by multiple staining for quantification of glycoprotein isoforms. Structural analysis of glycopeptides was performed with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), which provided very high mass accuracy and facilitated site-specific determination. A decreased glycosylation level for a protein localized in senile plaques, α 1 -antitrypsin, was found in AD patients. No specific glycoform of the studied proteins could be assigned to AD, emphasizing that further studies should include a larger subject group and cover proteins in various pH intervals. Knowledge of the respective glycoprotein structures in relation to clinical disease parameters may assist in the elucidation of the pathogenesis.In order to study proteins involved in the response of astrocytes and regenerative processes after neurotrauma, a quantitative mass spectrometric method was developed. Astrocytes, which are the most abundant cells in the central nervous system, react to neurotrauma by becoming reactive (reactive gliosis). Mice lacking the intermediate filament proteins GFAP and vimentin (GFAP -/-Vim -/-) show attenuated reactive gliosis and enhanced regeneration after neurotrauma. Comparative proteomic analysis showed upregulation of the adapter protein 14-3-3 in wildt...

show abstract

“…Recent detailed articles describing the proteomic advances made in neurological pathology, focusing mainly on neurodegenerative diseases, can be found in the literature [78,[217][218][219][220].…”

Section: Cerebrospinal Fluidmentioning

confidence: 99%

A better understanding of molecular mechanisms underlying human disease

Bermúdez-Crespo

López

2007

Proteomics Clinical Apps

View full text Add to dashboard Cite

This review summarises and discusses the degree to which proteomics is contributing to medical care, providing examples and signspots for future directions. Why do genomic approaches provide a limited view of gene expression? Because of the multifactorial nature of many diseases, proteomics enables us to understand the molecular basis of disease, not only at the organism, whole‐cell or tissue levels, but also in subcellular structures, protein complexes and biological fluids. The application of proteomics in medicine is expected to have a major impact by providing an integrated view of individual disease processes. This review describes several proteomic platforms and examines the role of proteomics as a tool for clinical biomarker discovery, the identification of prognostic and earlier diagnostic markers, their use in monitoring the effects of drug treatments and eventually find more efficient and safer therapeutics for a wide range of pathologies.

show abstract

Proteomics analysis of human cerebrospinal fluid

Cited by 108 publications

References 45 publications

Clinical proteomics of the cerebrospinal fluid: Towards the discovery of new biomarkers

Clinical proteomics of the cerebrospinal fluid: Towards the discovery of new biomarkers

Prefractionation of Cerebrospinal Fluid to Enhance Glycoprotein Concentration Prior to Structural Determination with FT−ICR Mass Spectrometry

A better understanding of molecular mechanisms underlying human disease

Contact Info

Product

Resources

About