A better understanding of molecular mechanisms underlying human disease

Bermúdez-Crespo, José; López, José Luis

doi:10.1002/prca.200700086

Cited by 21 publications

(19 citation statements)

References 267 publications

(248 reference statements)

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“…Although there are only about 23 000 human genes, it is difficult to realize all of the expressed human proteins, which mostly attributes to post translational modifications (PTMs), such as phosphorylation, glycosylation, ubiquitination, etc. 1, 2. Reversible protein phosphorylation is one of the most existing PTMs in organisms and it is crucial to many complex biological processes, such as cellular growth, division, and signal transduction 3–5.…”

Section: Introductionmentioning

confidence: 99%

Bioavailability of pharmaceuticals in waters close to wastewater treatment plants: Use of fish bile for exposure assessment

Lahti

Brozinski

Segner

et al. 2012

Enviro Toxic and Chemistry

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Pharmaceuticals are ubiquitous in surface waters as a consequence of discharges from municipal wastewater treatment plants. However, few studies have assessed the bioavailability of pharmaceuticals to fish in natural waters. In the present study, passive samplers and rainbow trout were experimentally deployed next to three municipal wastewater treatment plants in Finland to evaluate the degree of animal exposure. Pharmaceuticals from several therapeutic classes (in total 15) were analyzed by liquid chromatography-tandem mass spectrometry in extracts of passive samplers and in bile and blood plasma of rainbow trout held at polluted sites for 10 d. Each approach indicated the highest exposure near wastewater treatment plant A and the lowest near that of plant C. Diclofenac, naproxen, and ibuprofen were found in rainbow trout, and their concentrations in bile were 10 to 400 times higher than in plasma. The phase I metabolite hydroxydiclofenac was also detected in bile. Hence, bile proved to be an excellent sample matrix for the exposure assessment of fish. Most of the monitored pharmaceuticals were found in passive samplers, implying that they may overestimate the actual exposure of fish in receiving waters. Two biomarkers, hepatic vitellogenin and cytochrome P4501A, did not reveal clear effects on fish, although a small induction of vitellogenin mRNA was observed in trout caged near wastewater treatment plants B and C.

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Section: Introductionmentioning

confidence: 99%

Bioavailability of pharmaceuticals in waters close to wastewater treatment plants: Use of fish bile for exposure assessment

Lahti

Brozinski

Segner

et al. 2012

Enviro Toxic and Chemistry

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show abstract

“…After genomics, proteomics is often considered the next logical step to study biological systems, with the added capacity to describe the spatiotemporal differences in protein expression, both in normal and pathological tissue 18–20. The proteome represents all the proteins expressed by a genome, cell, tissue or organism at a given time under defined physiological conditions.…”

Section: Introductionmentioning

confidence: 99%

An Optimal Protocol to Analyze the Rat Spinal Cord Proteome

et al. 2009

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Since the function of the spinal cord depends on the proteins found there, better defing the normal Spinal Cord Proteome is an important and challenging task. Although brain and cerebrospinal fluid samples from patients with different central nervous system (CNS) disorders have been studied, a thorough examination of specific spinal cord proteins and the changes induced by injury or associated to conditions such as neurodegeneration, spasticity and neuropathies has yet to be performed. In the present study, we aimed to describe total protein content in the spinal cord of healthy rats, employing different proteomics tools. Accordingly, we have developed a fast, easy, and reproducible sequential protocol for protein extraction from rat spinal cords. We employed conventional two dimensional electrophoresis (2DE) in different pH ranges (eg. 4–7, 3–11 NL) combined with identification by mass spectrometry (MALDI-TOF/TOF), as well as first dimension protein separation combined with Liquid Chromatography Mass Spectrometry/Mass Spectrometry (LC-MS/MS) to maximise the benefits of this technology. The value of these techniques is demonstrated here by the identification of several proteins known to be associated with neuroglial structures, neurotransmission, cell survival and nerve growth in the central nervous system. Furthermore this study identified many spinal proteins that have not previously been described in the literature and which may play an important role as either sensitive biomarkers of dysfunction or of recovery after Spinal Cord Injury.

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“…In these years, a wide range of human diseases have been investigated using 2-DE technology (Hu et al, 2006;Bermúdez-Crespo and López, 2007). This can be attributed to the availability of commercial IPG strips, the use of MS to protein identification and more recently, the 2D-DIGE development.…”

Section: Biomedical Applications Of 2-dementioning

confidence: 99%

Possibilities of Two-Dimensional Gel Electrophoresis in the Understanding of Human Disease

Jose¹,

Bermúdez-Crespo²

2007

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Because of the multifactorial nature of many diseases, two-dimensional electrophoresis is a basic proteomics tool for its ability of simultaneously detecting post-and co-translational modifications, which cannot be predicted from genome sequences.This review describes the central role of proteomics tool, two-dimensional electrophoresis for clinical biomarker discovery, the identification of prognostic and diagnostic markers, their use in monitoring the effects of drug treatments and eventually finding more efficient and safer therapeutics for a wide range of pathologies.

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A better understanding of molecular mechanisms underlying human disease

Cited by 21 publications

References 267 publications

Bioavailability of pharmaceuticals in waters close to wastewater treatment plants: Use of fish bile for exposure assessment

Bioavailability of pharmaceuticals in waters close to wastewater treatment plants: Use of fish bile for exposure assessment

An Optimal Protocol to Analyze the Rat Spinal Cord Proteome

Possibilities of Two-Dimensional Gel Electrophoresis in the Understanding of Human Disease

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