Proteomic studies with a novel nano-magnetic chelating system to capture metalloproteins and its application in the preliminary study of monocyte and macrophage sub-secretome

Couto, Cláudia; Neves, Bruno Miguel; Ferreira, Rita; Daniel‐da‐Silva, Ana L.; Vitorino, Rui

doi:10.1016/j.talanta.2016.05.051

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…Alternatively, due to their effect on M1 phenotype activation in macrophages, various IONP formulations can also be explored as a possible vaccine adjuvant [44]. More recently, Couto et al developed an IONP-based chelating chromatography method to study metalloprotein secretion from monocytes and macrophages [45]. Although it is in a very early stage, this technology was found to be more efficient and cost-effective, and it may be expanded for broader proteomics and metabolomics applications.…”

Section: General Overview and Biomedical Application Of Iron-basedmentioning

confidence: 99%

Immunological effects of iron oxide nanoparticles and iron-based complex drug formulations: Therapeutic benefits, toxicity, mechanistic insights, and translational considerations

Shah

Dobrovolskaia

2018

Nanomedicine: Nanotechnology, Biology and Medicine

113

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Nanotechnology offers several advantages for drug delivery. However, there is the need for addressing potential safety concerns regarding the adverse health effects of these unique materials. Some such effects may occur due to undesirable interactions between nanoparticles and the immune system, and they may include hypersensitivity reactions, immunosuppression, and immunostimulation. While strategies, models, and approaches for studying the immunological safety of various engineered nanoparticles, including metal oxides, have been covered in the current literature, little attention has been given to the interactions between iron oxide-based nanomaterials and various components of the immune system. Here we provide a comprehensive review of studies investigating the effects of iron oxides and iron-based nanoparticles on various types of immune cells, highlight current gaps in the understanding of the structure-activity relationships of these materials, and propose a framework for capturing their immunotoxicity to streamline comparative studies between various types of iron-based formulations.

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Section: General Overview and Biomedical Application Of Iron-basedmentioning

confidence: 99%

Immunological effects of iron oxide nanoparticles and iron-based complex drug formulations: Therapeutic benefits, toxicity, mechanistic insights, and translational considerations

Shah

Dobrovolskaia

2018

Nanomedicine: Nanotechnology, Biology and Medicine

113

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“…Sensitive quantification of low-abundance proteins in the circulation is a technical challenge that was recently addressed with nanoparticle-based enrichment and subsequent mass spectrometry [91][92][93][94]. Moreover, enrichment of metalloproteins with magnetic chelating nanoparticles [95] or enrichment and detection of serum proteins with fluorescent nanocrystal microbeads coupled to a variety of antibodies [96] was described. An even more advanced technique with easy clinical application is the instrument-free, cost-effective method to enrich and colorimetrically detect phosphopeptides from serum samples within 15 min using TiO 2 -nanoparticle-based chemisorption and a tetrabromophenol assay [97].…”

Section: Circulating Proteinsmentioning

confidence: 99%

Combinatorial Power of cfDNA, CTCs and EVs in Oncology

Keup

Kasimir‐Bauer

2022

Diagnostics

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Liquid biopsy is a promising technique for clinical management of oncological patients. The diversity of analytes circulating in the blood useable for liquid biopsy testing is enormous. Circulating tumor cells (CTCs), cell-free DNA (cfDNA) and extracellular vesicles (EVs), as well as blood cells and other soluble components in the plasma, were shown as liquid biopsy analytes. A few studies directly comparing two liquid biopsy analytes showed a benefit of one analyte over the other, while most authors concluded the benefit of the additional analyte. Only three years ago, the first studies to examine the value of a characterization of more than two liquid biopsy analytes from the same sample were conducted. We attempt to reflect on the recent development of multimodal liquid biopsy testing in this review. Although the analytes and clinical purposes of the published multimodal studies differed significantly, the additive value of the analytes was concluded in almost all projects. Thus, the blood components, as liquid biopsy reservoirs, are complementary rather than competitive, and orthogonal data sets were even shown to harbor synergistic effects. The unmistakable potential of multimodal liquid biopsy testing, however, is dampened by its clinical utility, which is yet to be proven, the lack of methodical standardization and insufficiently mature reimbursement, logistics and data handling.

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Recent advances in sample pre-treatment for emerging methods in proteomic analysis

Ramos‐Payán

Ocaña-González

Fernández‐Torres

et al. 2017

Talanta

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Proteomic studies with a novel nano-magnetic chelating system to capture metalloproteins and its application in the preliminary study of monocyte and macrophage sub-secretome

Cited by 3 publications

References 17 publications

Immunological effects of iron oxide nanoparticles and iron-based complex drug formulations: Therapeutic benefits, toxicity, mechanistic insights, and translational considerations

Immunological effects of iron oxide nanoparticles and iron-based complex drug formulations: Therapeutic benefits, toxicity, mechanistic insights, and translational considerations

Combinatorial Power of cfDNA, CTCs and EVs in Oncology

Recent advances in sample pre-treatment for emerging methods in proteomic analysis

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