Recent advances in sample pre-treatment for emerging methods in proteomic analysis

Ramos‐Payán, María; Ocaña-González, Juan Antonio; Fernández‐Torres, Rut; Maspoch, S.; Bello‐López, Miguel Ángel

doi:10.1016/j.talanta.2017.06.056

Cited by 5 publications

(3 citation statements)

References 123 publications

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“…Compared to the conventional IMAC technique, various micro/nanoparticles including 602 magnetic core-mesoporous shell variants, mesoporous SiO 2 supported nanocomposites show promise [54]. On one hand, the synthesized novel sorbents mostly exhibit excellent performances for p-peptide enrichment due to the intrinsic and robust magnetic properties, which were beneficial for rapid enrichment and separation of p-peptides.…”

Section: Developments Includementioning

confidence: 99%

Phosphopeptide enrichment for phosphoproteomic analysis - A tutorial and review of novel materials

Qiu

Evans

Landels

et al. 2020

Analytica Chimica Acta

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Post-translational modifications (PTMs), mass spectrometry (MS), liquid chromatography (LC), tandem MS (MS/MS), phosphopeptide (p-peptide), label-free quantification (LFQ), stable isotope labelling by amino acids in cell culture (SILAC), Tandem Mass Tags (TMT) isobaric tags for relative and absolute quantification (iTRAQ), phospho-serine (pSer), threonine (pThr), tyrosine (pTyr), immobilized metal ion affinity chromatography (IMAC), metal oxide affinity chromatography (MOAC), acetonitrile (ACN), sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS-PAGE), filter assisted sample preparation (FASP), solid phase extraction (SPE), high performance liquid chromatography (HPLC), reverse phase (RP), strong cation exchange (SCX), electrostatic repulsion hydrophilic interaction chromatography (ERLIC), solution isoelectric focusing (sIEF), strong anion exchange (SAX), histidine (pHis), arginine (Arg),

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Section: Developments Includementioning

confidence: 99%

Phosphopeptide enrichment for phosphoproteomic analysis - A tutorial and review of novel materials

Qiu

Evans

Landels

et al. 2020

Analytica Chimica Acta

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“…Therefore, phosphorylation research is of great significance; however, analysis of intact phosphoproteins in biological samples remains a great challenge because of low abundance, low stoichiometry, and suppression from nonphosphoproteins which often has higher sensitivity. Hence, enrichment procedure prior to analysis is a prerequisite .…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, phosphorylation research is of great significance; however, analysis of intact phosphoproteins in biological samples remains a great challenge because of low abundance, low Article Related Abbreviations: ACN, acetonitrile; ATP, adenosine triphosphates; GMA, glycidyl methacrylate; IMAC, immobilized metal-ion affinity chromatography; MP, microsphere; OVA, ovalbumin; PGMA, poly-glycidyl methacrylate stoichiometry, and suppression from nonphosphoproteins which often has higher sensitivity. Hence, enrichment procedure prior to analysis is a prerequisite [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Facile synthesis of titanium(IV) ion‐immobilized poly‐glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate for highly specific enrichment of intact phosphoproteins

Wang

Tian

2018

J of Separation Science

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In this study, a facile synthesis of the titanium(IV) ion‐immobilized poly‐glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate was developed for efficient enrichment of intact phosphoproteins. The titanium(IV) ion‐immobilized microparticles had higher saturated adsorption capacity for phosphoproteins (1217.6 mg/g for β‐casein) than nonphosphoproteins (97.1 mg/g for bovine serum albumin) and the average particle diameter was about 1.4 μm with good dispersibility. In application, as demonstrated by SDS‐PAGE, titanium(IV) ion‐immobilized microparticles exhibited good performance in enriching intact phosphoproteins from standard protein mixtures of β‐casein and bovine serum albumin with high specificity and selectivity. In addition, titanium(IV) ion‐immobilized microparticles were also successfully applied in intact phosphoprotein enrichment from complex biological samples including nonfat milk, chicken egg white, and mouse heart tissue extract.

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Cook Your Samples: The Application of Microwave Irradiation in Speeding Up Biological Processes

Chen

Liu

et al. 2018

Mol Biotechnol

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Classic and conventional procedures in molecular cloning are inherent compositions in modern molecular biological experiments and are frequently involved in daily laboratory activities. They take up the majority of the total time input in spite of the availability of well-designed specialized commercial kits. A similar situation is also in the field of biotechnology. Fortunately, microwave/ultrasonic irradiation has been found to be capable of speeding up these processes, such as proteolysis in sample preparation for proteomics research, and digestion, ligation, (de)phosphorylation of DNA with the corresponding enzymes, even the introduction of DNA samples to recipient cells, and biotransformation (e.g., the production of biodiesel). Microwave/ultrasonic irradiation, when used solely or in combination with other existing operations, makes it possible to finish these time-consuming processes in as short as 1 min with comparable or even improved efficiency, and there is no need of reagent upgradation. The adoption of irradiation is ideal because it eliminates any possible side effects of the chemicals used as performance enhancer(s) that will inevitably make the system more complicated at least. More notably, the needed irradiation in the laboratory can be generated by a common microwave oven or ultrasonic cleaner. Taken together, microwave/ultrasonic irradiation provides an accessible method to make the procedures mentioned above time- and cost- efficient. In this article, we reviewed the relevant literature and discussed the experiment and mechanism details.

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Recent advances in sample pre-treatment for emerging methods in proteomic analysis

Cited by 5 publications

References 123 publications

Phosphopeptide enrichment for phosphoproteomic analysis - A tutorial and review of novel materials

Phosphopeptide enrichment for phosphoproteomic analysis - A tutorial and review of novel materials

Facile synthesis of titanium(IV) ion‐immobilized poly‐glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate for highly specific enrichment of intact phosphoproteins

Cook Your Samples: The Application of Microwave Irradiation in Speeding Up Biological Processes

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