Proteomic signature of muscle fibre hyperplasia in response to faba bean intake in grass carp

Abstract

Fish muscle growth is important for the rapidly developing global aquaculture industry, particularly with respect to production and quality. Changes in muscle fibre size are accomplished by altering the balance between protein synthesis and proteolysis. However, our understanding regarding the effects of different protein sources on fish muscle proteins is still limited. Here we report on the proteomic profile of muscle fibre hyperplasia in grass carp fed only with whole faba bean. From the results, a total of 99 significantly changed proteins after muscle hyperplasia increase were identified (p < 0.05, ratio <0.5 or >2). Protein–protein interaction analysis demonstrated the presence of a network containing 56 differentially expressed proteins, and muscle fibre hyperplasia was closely related to a protein–protein network of 12 muscle component proteins. Muscle fibre hyperplasia was also accompanied by decreased abundance in the fatty acid degradation and calcium signalling pathways. In addition, metabolism via the pentose phosphate pathway decreased in grass carp after ingestion of faba bean, leading to haemolysis. These findings could provide a reference for the prevention and treatment of human glucose-6-phosphate dehydrogenase deficiency (“favism”).

Feeding Faba Beans (Vicia faba L.) Reduces Myocyte Metabolic Activity in Grass Carp (Ctenopharyngodon idellus)

“…Though many studies have explored the molecular changes in FBFGs, the mechanism of increased "hardness" in the muscle of this type of grass carp was not fully understood (Yu et al, 2017). It was suggested that fiber density was one of the main reasons for the observed change in muscle texture, which was due to the attenuation of myocyte hyperplasia (Yu et al, 2017).…”

“…The samples of fixed muscle were washed in tap water for 12 h, followed by a routine dehydration in a graded series of ethanol (30, 50, 70, 80, 90, 95, and 100% twice). Samples were then equilibrated in xylene and embedded in paraffin based on standard histological techniques previously described (Yu et al, 2017). Afterward, 5-µm sections were cut with a rotary microtome (RM2235, Leica, Germany), mounted on glass slides, and stained with H&E. Histological samples were observed and photographed using an upright microscope (Leica biosystems, Wetzlar, Germany).…”

“…Compared with commercial diet fed-grass carp (CDFG), FBFG needs a strict culture environment of fresh water with high dissolved oxygen (Xie et al, 2012). Moreover, FBFG presents lower growth and swimming motility, as well as higher mortality than CDFG (Yu et al, 2017;Tian et al, 2019). Interestingly, faba bean readily induces fat accumulation in the visceral tissues (such as the adipose tissue and hepatopancreas) compared with commercial diets, suggesting an unreleased energy status in FBFG.…”

Safety evaluation of four faba bean extracts used as dietary supplements in grass carp culture based on hematological indices, hepatopancreatic function and nutritional condition

“…(Clauss, Dove & Arnold, 2008;Fazio, 2018). It has been reported that ingestion of faba bean leads to hemolysis, which results in reduced red blood cell count (RBC) and decreased hemoglobin content, and thereby increases the oxygen requirement in grass carp (Yu et al, 2017). Our previous study has found that the FB and FB water extract groups have low RBC and glucose-6-phosphate dehydrogenase (G6PD) activity, which possibly causes hemolysis (Ma et al, 2020).…”

Broad Bean (Vicia faba L.) Induces Intestinal Inflammation in Grass Carp (Ctenopharyngodon idellus C. et V) by Increasing Relative Abundances of Intestinal Gram-Negative and Flagellated Bacteria

“…In 2012, the harvest volume of GCBB was over 22,100 tons in Guangdong Province, China ( Zhang et al, 2015 ). GCBB exhibits increased muscle hardness and crispiness as compared to normal grass carp ( Yu et al, 2017 ) due to a 36.7% higher collagen content in muscle ( Liu et al, 2011 ). However, constant consumption of broad bean by the carp causes intestinal mucosal injury resulting from perpetual inflammation ( Zhang, 2015 ), although the underlying mechanism is unclear.…”