Broad Bean (Vicia faba L.) Induces Intestinal Inflammation in Grass Carp (Ctenopharyngodon idellus C. et V) by Increasing Relative Abundances of Intestinal Gram-Negative and Flagellated Bacteria

Li, Zhifei; Yu, Ermeng; Wang, Guangjun; Yu, Deguang; Zhang, Kai; Gong, Wangbao; Xie, Jun

doi:10.3389/fmicb.2018.01913

Cited by 19 publications

(17 citation statements)

References 53 publications

(61 reference statements)

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“…Interestingly, faba bean has been suggested to cause intestinal damage and induce inflammation in grass carps (Li et al, 2018), and wounds have been reported to attract fat cells to drive repair and prevent infection (Franz et al, 2018). However, further studies are needed to determine whether intestinal damage or inflammation can induce fat accumulation in peri-intestinal tissues of fish.…”

Section: Discussionmentioning

confidence: 99%

Feeding Faba Beans (Vicia faba L.) Reduces Myocyte Metabolic Activity in Grass Carp (Ctenopharyngodon idellus)

Tian

et al. 2020

Front. Physiol.

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In this study, we aimed to explore the effects of faba bean (Vicia faba L.) on the energy metabolism of grass carp (Ctenopharyngodon idellus). A total of 180 fish (∼2900 g) were randomly assigned to six tanks (2.5 × 2.5 × 1.2 m; 30 individuals per tank) and fed either faba bean (Vicia faba L.) or a commercial diet for 120 days (3% body weight, twice per day). The results showed that faba bean-fed grass carp (FBFG) had significantly lower growth and higher fat accumulation in the mesenteric adipose tissue and hepatopancreas than commercial diet-fed grass carp (CDFG). Compared with CDFG, FBFG exhibited no significant difference in proximate composition of the muscle; however, an obvious decrease in muscle fiber size and significantly higher hardness, chewiness, and gumminess were observed. Transcriptome results showed that a total of 197 genes were differentially regulated in the dorsal muscle. Down-regulated genes included four genes annotated with myocyte development and 12 transcripts annotated with components of myofibrils. In addition, the FBFG group exhibited significantly lower expression of genes associated with oxygen transport, the mitochondrial respiratory chain, and creatine metabolism, suggesting reduced energy availability in the muscle of the FBFG. Moreover, using western-blotting and enzyme assays, we found decreased protein levels in the mitochondrial electron transport respiratory chain and creatine metabolism activities, as well as increased expression of autophagy marker protein levels, in the muscle of FBFG. Overall, our results suggest that an abnormal energy distribution may exist in grass carps after feeding with faba bean, which is reflected by a mass of fat deposition in the adipose tissue and hepatopancreas and subdued metabolic activity in the muscle.

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Section: Discussionmentioning

confidence: 99%

Feeding Faba Beans (Vicia faba L.) Reduces Myocyte Metabolic Activity in Grass Carp (Ctenopharyngodon idellus)

Tian

et al. 2020

Front. Physiol.

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“…These antinutritional factors reduced the activity of digestive enzymes (Li, Leng, Li, Liu, et al, ; Zheng et al, ) and thus negatively affected the digestion and absorption of nutrients. Moreover, broad bean was reported to induce intestinal inflammation in grass carp (Li et al, ). In the present trial, grass carp fed SBB were found to consume the diet slowly, and some fish even vomited after eating during the feeding period.…”

Section: Discussionmentioning

confidence: 99%

The supplementation of nutrient additives in broad bean‐based diet improved the growth of “crisped” grass carp, Ctenopharyngodon idellus

Weibo

et al. 2019

J World Aquaculture Soc

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To investigate the effects of supplemental nutrient additives in broad bean‐based diets on growth, flesh quality, and collagen gene expression of grass carp, five diets were prepared, including complete formula diet (control), soaked broad bean (SBB), and three broad bean‐based diets containing 80% broad bean without (BBD1) or with the supplementation of methionine (BBD2), and methionine+vitamins+minerals (BBD3), and were fed to grass carp (171.9 ± 1.1 g), Ctenopharyngodon idellus, for 84 days. The results showed that broad bean‐based diets significantly increased weight gain (WG) and reduced the feed conversion ratio (FCR) of grass carp when compared to the SBB (p < .05). The BBD3 group reached levels similar to the WG and FCR of the control group (p > .05). Grass carp fed the BBD3 diet had lower steaming loss of flesh, higher muscle fiber density, and higher collagen content in muscle and skin than the control (p < .05). The relative expressions of COL1A1 and COL1A2 mRNA in muscle and skin were significantly higher in the BBD3 and SBB groups than in other groups (p < .05). In conclusion, the combination of methionine, vitamins, and minerals in broad bean‐based diets promoted the growth of “crisped” grass carp and improved flesh quality and collagen gene expression when compared to the control formula diet.

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“…The values were calculated through the R indicspecies package. Screened genera were verified using the standard non-parametric Kruskal–Wallis test through R with the ggpubr package according to a previous report (Li et al, 2018). The Kruskal–Wallis test was also used to detect the statistical significance of alpha-diversity indices among patients with different stages of HCC and healthy controls.…”

Section: Methodsmentioning

confidence: 99%

Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma

Huang

Zhou

et al. 2019

Front. Microbiol.

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Gut microbiota dysbiosis is closely associated with primary hepatocellular carcinoma (HCC). Recent studies have evaluated the early diagnosis of primary HCC through analysis of gut microbiota dysbiosis. However, the relationship between the degree of dysbiosis and the prognosis of primary HCC remains unclear. Because primary HCC is accompanied by dysbiosis and dysbiosis usually increases the circulatory concentrations of endotoxin and other harmful bacterial substances, which further increases liver damage, we hypothesized that level of dysbiosis associated with primary HCC increases with the stage of cancer progression. To test this hypothesis, we introduced a more integrated index referred to as the degree of dysbiosis ( D dys ); and we investigated D dys of the gut microbiota with the development of primary HCC through high-throughput sequencing of 16S rRNA gene amplicons. Our results showed that compared with healthy individuals, patients with primary HCC showed increased pro-inflammatory bacteria in their fecal microbiota. The D dys increased significantly in patients with primary HCC compared with that in healthy controls. Moreover, there was a tendency for the D dys to increase with the development of primary HCC, although no significant difference was detected between different stages of primary HCC. Our findings provide important insights into the use of gut microbiota analysis during the treatment of primary HCC.

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Broad Bean (Vicia faba L.) Induces Intestinal Inflammation in Grass Carp (Ctenopharyngodon idellus C. et V) by Increasing Relative Abundances of Intestinal Gram-Negative and Flagellated Bacteria

Cited by 19 publications

References 53 publications

Feeding Faba Beans (Vicia faba L.) Reduces Myocyte Metabolic Activity in Grass Carp (Ctenopharyngodon idellus)

Feeding Faba Beans (Vicia faba L.) Reduces Myocyte Metabolic Activity in Grass Carp (Ctenopharyngodon idellus)

The supplementation of nutrient additives in broad bean‐based diet improved the growth of “crisped” grass carp, Ctenopharyngodon idellus

Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma

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