Proteomic response of gill microsomes of Crassostrea brasiliana exposed to diesel fuel water-accommodated fraction

Müller, Gabrielle do Amaral e Silva; Lüchmann, Karim Hahn; Razzera, Guilherme; Toledo-Silva, Guilherme; Bebianno, Maria João; Marques, Maria Risoleta Freire; Bainy, Afonso Celso Dias

doi:10.1016/j.aquatox.2018.06.001

Cited by 8 publications

(4 citation statements)

References 85 publications

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“…The network analysis of lower abundant proteins highlights the disruption of the "glutamate biosynthetic process" and, interestingly, glutamate synthase (FC, 0.018; FDR, 0.082) was found among the top five of less abundant proteins in this tissue pool. Its decrease decreases glutamate synthesis (from 2-oxoglutarate, an intermediate of the tricarboxylic acid cycle), which is in agreement with results from Müller et al (2018) on oysters. These authors observed a decrease in glutamate 4 receptor abundance in gill microsomes of oysters Crassostrea brasiliana exposed during 24 h to water contaminated by diesel fuel.…”

Section: Proteins With a Lower Abundancesupporting

confidence: 90%

Proteome changes in muscles, ganglia, and gills in Corbicula fluminea clams exposed to crude oil: Relationship with behavioural disturbances

et al. 2020

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Section: Proteins With a Lower Abundancesupporting

confidence: 90%

Proteome changes in muscles, ganglia, and gills in Corbicula fluminea clams exposed to crude oil: Relationship with behavioural disturbances

et al. 2020

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“…Specifically, enrichment was seen among hypomethylated genes coding for a gene with enzyme annotation of glycosyltransferase and the gene coding for a glutathione synthetase-like protein. These enzymes are important for the biosynthesis of many carbohydrates and detoxification that have been shown to be differentially regulated, at the transcript level, in response to stress in other Crassostrea species (Müller et al, 2018;Tanguy, Boutet, & Moraga, 2005). The hypermethylation of ketohexokinase, an enzyme involved in metabolism, suggests that differences in food availability between the estuaries may influence these methylation patterns.…”

Section: Dna Methylation and Population Structurementioning

confidence: 99%

Population epigenetic divergence exceeds genetic divergence in the Eastern oyster Crassostrea virginica in the Northern Gulf of Mexico

Johnson

Kelly

2020

Evolutionary Applications

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Evolutionary Applications. 2020;13:945-959. | 945 wileyonlinelibrary.com/journal/eva AbstractPopulations may respond to environmental heterogeneity via evolutionary divergence or phenotypic plasticity. While evolutionary divergence occurs through DNA sequence differences among populations, plastic divergence among populations may be generated by changes in the epigenome. Here, we present the results of a genome-wide comparison of DNA methylation patterns and genetic structure among four populations of Eastern oyster (Crassostrea virginica) in the northern Gulf of Mexico. We used a combination of restriction site-associated DNA sequencing (RADseq) and reduced representation bisulfite sequencing (RRBS) to explore population structure, gene-wide averages of F ST , and DNA methylation differences between oysters inhabiting four estuaries with unique salinity profiles. This approach identified significant population structure despite a moderately low F ST (0.02) across the freshwater boundary of the Mississippi river, a finding that may reflect recent efforts to restore oyster stock populations. Divergence between populations in CpG methylation was greater than for divergence in F ST , likely reflecting environmental effects on DNA methylation patterns. Assessment of CpG methylation patterns across all populations identified that only 26% of methylated DNA was intergenic; and, only 17% of all differentially methylated regions (DMRs) were within these same regions.DMRs within gene bodies between sites were associated with genes known to be involved in DNA damage repair, ion transport, and reproductive timing. Finally, when assessing the correlation between genomic variation and DNA methylation between these populations, we observed population-specific DNA methylation profiles that were not directly associated with single nucleotide polymorphisms or broader genebody mean F ST trends. Our results suggest that C. virginica may use DNA methylation to generate environmentally responsive plastic phenotypes and that there is more divergence in methylation than divergence in allele frequencies. K E Y W O R D SCrassostrea virginica, DNA methylation, epigenetics, oyster, reduced representation bisulfite sequencing, salinity

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“…A gene encoding tubulin β chain was also downregulated under EE with respect to AA and EA. Tubulin is a major constituent of microtubules and is associated with the cytoskeleton [ 74 ]. In Müller et al (2018) [ 74 ], Crassostrea brasiliana exposed to a diesel fuel water-accommodated fraction differentially regulated α and β tubulin.…”

Section: Discussionmentioning

confidence: 99%

“…Tubulin is a major constituent of microtubules and is associated with the cytoskeleton [ 74 ]. In Müller et al (2018) [ 74 ], Crassostrea brasiliana exposed to a diesel fuel water-accommodated fraction differentially regulated α and β tubulin. This differential expression was cited to be the result of oxidative stress, which could explain the downregulation in larvae here.…”

Section: Discussionmentioning

confidence: 99%

A Transcriptomic Analysis of Phenotypic Plasticity in Crassostrea virginica Larvae under Experimental Acidification

Barbosa

Schwaner

Espinosa

et al. 2022

Genes

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Ocean acidification (OA) is a major threat to marine calcifiers, and little is known regarding acclimation to OA in bivalves. This study combined physiological assays with next-generation sequencing to assess the potential for recovery from and acclimation to OA in the eastern oyster (Crassostrea virginica) and identify molecular mechanisms associated with resilience. In a reciprocal transplant experiment, larvae transplanted from elevated pCO2 (~1400 ppm) to ambient pCO2 (~350 ppm) demonstrated significantly lower mortality and larger size post-transplant than oysters remaining under elevated pCO2 and had similar mortality compared to those remaining in ambient conditions. The recovery after transplantation to ambient conditions demonstrates the ability for larvae to rebound and suggests phenotypic plasticity and acclimation. Transcriptomic analysis supported this hypothesis as genes were differentially regulated under OA stress. Transcriptomic profiles of transplanted and non-transplanted larvae terminating in the same final pCO2 converged, further supporting the idea that acclimation underlies resilience. The functions of differentially expressed genes included cell differentiation, development, biomineralization, ion exchange, and immunity. Results suggest acclimation as a mode of resilience to OA. In addition, the identification of genes associated with resilience can serve as a valuable resource for the aquaculture industry, as these could enable marker-assisted selection of OA-resilient stocks.

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Proteomic response of gill microsomes of Crassostrea brasiliana exposed to diesel fuel water-accommodated fraction

Cited by 8 publications

References 85 publications

Proteome changes in muscles, ganglia, and gills in Corbicula fluminea clams exposed to crude oil: Relationship with behavioural disturbances

Proteome changes in muscles, ganglia, and gills in Corbicula fluminea clams exposed to crude oil: Relationship with behavioural disturbances

Population epigenetic divergence exceeds genetic divergence in the Eastern oyster Crassostrea virginica in the Northern Gulf of Mexico

A Transcriptomic Analysis of Phenotypic Plasticity in Crassostrea virginica Larvae under Experimental Acidification

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