Proteomic profiling of follicle fluids after superstimulation in one‐month‐old lambs

Abstract

Follicular fluid (FF) accumulates in the antrum of the ovarian follicle. In addition, FF provides the microenvironment for oocyte development, oocyte maturation and competence, which are acquired during follicular development. Superstimulatory treatment of 1-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles are unable to completely mature and ovulate. Furthermore, the oocytes exhibit lower competence compared with those of ewes. In this study, we utilized an isobaric tag for relative and absolute quantification (iTRAQ)-based proteomics analysis and compare protein composition between pre-pubertal and adult superstimulated follicle FF in sheep. In total, 243 differentially expressed proteins were identified, including 155 downregulated and 88 upregulated between lamb and ewe. Gene ontology (GO) and KEGG pathway analysis indicated that the differentially expressed proteins are involved in signal transduction, anatomical structure development, stress response, metabolic pathways, and the complement and coagulation cascades. Many of the proteins known to affect follicle development were observed in lower abundance in FF of lamb (e.g. ADAMTS9, CD14, CTNNB1, FST, GCLC, HSPG2, IGFBP2, IGFBP6, INHBA, PRL, PAPPA, POSTN, PRDX1, SERPINA1, SOD3, STC1, VEGFC, etc.). However, a higher abundance was observed for proteasome proteins. Inadequate amounts of these proteins in FF may be lead to the unique characteristics of follicular development in lamb. These differentially expressed proteins illuminate the age-dependent changes in protein expression in the follicle microenvironment.

Differential expression and prediction of function of lncRNAs in the ovaries of low and high fecundity Hanper sheep

“…Until the corpus luteum begins to shrink after the middle luteal phase, the expression of VEGF remains low or is even undetectable (Kuokkanen et al., 2016). The increase in VEGF prior to ovulation may reflect the proliferation and maturation of granulosa cells and follicular membrane cells and is possibly an indicator of the maturation of granulosa and follicular membrane cells (Wu et al., 2018). In the present study, the expression levels of VEGF in sheep that have had multiple lambs in the LM versus LP group were higher than those that are in single lambs.…”

Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814)

“…Proteins derived from blood serum (albumin, serotransferrin, inter-alpha-trypsin, haptoglobin, apolipoprotein and ceruloplasmin) were found in M. gouazoubira and had previously been described in FF of other species, such as bubaline (Fu et al, 2016), bovine (Ferrazza et al, 2017), caprine (Paula Junior et al, 2018) and ovine (Wu et al, 2018). During the final stage of folliculogenesis, the composition of FF is influenced by the blood-follicle barrier, which allows passage of low-molecularweight proteins .…”

“…The determination of follicular fluid (FF) composition may be important to ascertain the microenvironment involved in follicle development and as an indication of the nutritional needs of the oocyte. In addition, selection of competent oocytes requires the identification of molecular markers present in the FF , Several studies on the proteome of FF have been performed in bovine (Mortarino et al, 1999), canine , equine (Fahiminiya et al, 2011), swine (Sun et al, 2011), bubaline (Fu et al, 2016) and ovine (Wu et al, 2018).…”

The Roles of the miRNAome and Transcriptome in the Ovine Ovary Reveal Poor Efficiency in Juvenile Superovulation

“…Nevertheless, embryos derived from JIVET exhibit a significantly lower survival rate [38]. The maturation status of the cumulus cell [39], oocyte cytoplasm [40], oocyte nuclear [41], and follicular fluid microenvironment in juvenile ovaries [42][43][44] have been suggested to explain the poor efficiency of JIVET. In the present study, we focused on the initial stage of JIVET: ovaries under the treatment of superovulation; therefore, the miRNAome and transcriptome were compared between juvenile and adult ovaries to identify the mechanism of inferior oocyte capture by JIVET.…”