Proteomic profiling of cellular targets of lipopolysaccharide-induced signalling in Nicotiana tabacum BY-2 cells

Molecular characterisation and regulation of a Nicotiana tabacum S-domain receptor-like kinase gene induced during an early rapid response to lipopolysaccharides

“…This LPS was found to trigger a rapid influx of Ca 2+ into the cytoplasm of tobacco cells, as well as the production of reactive oxygen and nitrogen species (ROS and NO) during an oxidative burst reaction and K + /H + exchange and alkalization of the extracellular culture medium . LPS also has specific effects on reversible protein phosphorylation events underlying the perception systems involved in its interaction of plant cells Gerber et al, 2006Gerber et al, , 2008, e.g. the phosphorylation and activation of an extracellular signal-related MAP kinase (Piater et al, 2004).…”

Negative feedback regulation of microbe-associated molecular pattern-induced cytosolic Ca2+ transients by protein phosphorylation

“…Biochemical and genetical evidence indicates important roles of various layers of protein phosphorylation in defense signaling (Benschop et al 2007;Gerber et al 2008;He et al 2004;Nühse et al 2004). Typical MAMPs/ PAMPs such as flg22 are perceived by receptor protein kinases such as FLS2 (Zipfel et al 2004).…”

Metabolomic insights into the bioconversion of isonitrosoacetophenone in Arabidopsis thaliana and its effects on defense-related pathways

“…Several promoters for genes encoding proteins functioning towards plant defense responses, such as that of PR-1, are known to respond to chemical activators of defense (Padidam, 2003). Using bacterial lipopolysaccharide (LPS) as an inducer of SAR (Coventry and Dubery, 2001), we have shown that both transcriptomics (Sanabria and Dubery, 2006;Madala et al, 2012a) and proteomics (Piater et al, 2004;Gerber et al, 2006Gerber et al, , 2008 contribute to a comprehensive understanding of activation of plant defense responses. Recently, however, it was also demonstrated that metabolomics, a science of systems biology, can be used in this regard (Beets et al, 2012;Tugizimana et al, 2012;James et al, 2013).…”