Molecular characterisation and regulation of a Nicotiana tabacum S-domain receptor-like kinase gene induced during an early rapid response to lipopolysaccharides

Sanabria, Natasha; Heerden, Henriëtte van; Dubery, Ian A.

doi:10.1016/j.gene.2012.03.073

Cited by 40 publications

(53 citation statements)

References 65 publications

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“…RNA was reverse transcribed using Transcriptor Reverse Transcriptase (Roche) with equal concentrations of the Oligo‐dT primer and random hexamers. Similar to our previous report , 18S rRNA was the only stable reference gene during the LPS treatments. Experiments were designed to amplify two products in the PCR reactions.…”

Section: Methodssupporting

confidence: 90%

Alternative splicing of the receptor‐like kinase Nt‐Sd‐RLK in tobacco cells responding to lipopolysaccharides: suggestive of a role in pathogen surveillance and perception?

Sanabria

Dubery

2016

FEBS Letters

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Nt-Sd-RLK encodes an S-domain lectin receptor-like kinase that is induced in response to microbe-associated molecular pattern molecules (MAMPs) such as lipopolysaccharide (LPS). In this study, we investigated the alternative splicing of Nt-Sd-RLK in response to LPS stimulation. Our data indicate that in nonstimulated cells, a shorter transcript of Nt-Sd-RLK is generated and that in response to LPS, alternative splicing produces the full-length transcript. We propose that the extracellular domain of Nt-Sd-RLK encoded by the shorter transcript functions in pathogen surveillance. Once this domain binds LPS, alternative splicing generates the kinase domain-containing Nt-Sd-RLK that activates downstream signalling leading to a defence response. Thus, our findings suggest that plant defence signalling may be regulated through the alternative splicing of receptor-like kinases involved in pathogen recognition.

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Section: Methodssupporting

confidence: 90%

Alternative splicing of the receptor‐like kinase Nt‐Sd‐RLK in tobacco cells responding to lipopolysaccharides: suggestive of a role in pathogen surveillance and perception?

Sanabria

Dubery

2016

FEBS Letters

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“…Of note within the genes associated with a kinase function was the up‐regulation of a lectin protein kinase (Potri.004G027300). Lectin receptor‐like kinases, as part of the PRR family of proteins (De Felice & Wilson, ), are transcriptionally induced upon interaction with mutualistic fungi (Gaude et al ., ; Sanabria et al ., ), indicating that these receptors form another important component in the interaction of plant tissues with mutualistic organisms. Further, a homolog of CLAVATA3 (Potri.008G191500) and a cysteine‐rich receptor‐like kinase (Potri.004G024000), which have characterized roles in the perception and signaling during pathogenic plant–microbe interactions (Rayapuram et al ., ; Schwessinger & Ronald, ; Singh et al ., ), were found to be up‐regulated during the interaction.…”

Section: Resultsmentioning

confidence: 99%

Ethylene and jasmonic acid act as negative modulators during mutualistic symbiosis between Laccaria bicolor and Populus roots

et al. 2014

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SummaryThe plant hormones ethylene, jasmonic acid and salicylic acid have interconnecting roles during the response of plant tissues to mutualistic and pathogenic symbionts.We used morphological studies of transgenic-or hormone-treated Populus roots as well as whole-genome oligoarrays to examine how these hormones affect root colonization by the mutualistic ectomycorrhizal fungus Laccaria bicolor S238N.We found that genes regulated by ethylene, jasmonic acid and salicylic acid were regulated in the late stages of the interaction between L. bicolor and poplar. Both ethylene and jasmonic acid treatments were found to impede fungal colonization of roots, and this effect was correlated to an increase in the expression of certain transcription factors (e.g. ETHYLENE RESPONSE FACTOR1) and a decrease in the expression of genes associated with microbial perception and cell wall modification. Further, we found that ethylene and jasmonic acid showed extensive transcriptional cross-talk, cross-talk that was opposed by salicylic acid signaling.We conclude that ethylene and jasmonic acid pathways are induced late in the colonization of root tissues in order to limit fungal growth within roots. This induction is probably an adaptive response by the plant such that its growth and vigor are not compromised by the fungus.

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“…For example, Pi-d2 , a G-type lectin receptor kinase from rice, provides resistance against the fungal pathogen Magnaporthe grisea , the causal agent of rice blast (Chen et al, 2006). In tobacco, the expression of another G-type lectin receptor kinase was recently shown to be up-regulated by lipopolysaccharides (Sanabria et al, 2012). In Nicotiana benthamiana, the LecRK NbLRK1 was suggested to be a component of the N. benthamiana protein complex that recognizes the Phytophthora infestans INF1 elicitor and mediates INF1-induced cell death (Kanzaki et al, 2008).…”

Section: Lectin Receptor Kinases In Plant Defensementioning

confidence: 99%

Lectin receptor kinases in plant innate immunity

Singh¹,

Zimmerli²

2013

Front. Plant Sci.

110

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A key feature of innate immunity is the ability to recognize and respond to potential pathogens in a highly sensitive and specific manner. In plants, the first layer of defense is induced after recognition by pattern recognition receptors of microbe-associated molecular patterns. This recognition elicits a defense program known as pattern-triggered immunity. Pathogen entry into host tissue is a critical early step in causing infection. For foliar bacterial pathogens, natural surface openings such as stomata, are important entry sites. Stomata in contact with bacteria rapidly close and can thus restrict bacterial entry into leaves. The molecular mechanisms regulating stomatal closure upon pathogen perception are not yet well-understood. Plant lectin receptor kinases are thought to play crucial roles during development and in the adaptive response to various stresses. Although the function of most plant lectin receptor kinases is still not clear, a role for this kinase family in plant innate immunity is emerging. Here, we summarize recent progresses in the identification of lectin receptor kinases involved in plant innate immunity. We also discuss the role of lectin receptor kinases in stomatal innate immunity signaling.

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Molecular characterisation and regulation of a Nicotiana tabacum S-domain receptor-like kinase gene induced during an early rapid response to lipopolysaccharides

Cited by 40 publications

References 65 publications

Alternative splicing of the receptor‐like kinase Nt‐Sd‐RLK in tobacco cells responding to lipopolysaccharides: suggestive of a role in pathogen surveillance and perception?

Alternative splicing of the receptor‐like kinase Nt‐Sd‐RLK in tobacco cells responding to lipopolysaccharides: suggestive of a role in pathogen surveillance and perception?

Ethylene and jasmonic acid act as negative modulators during mutualistic symbiosis between Laccaria bicolor and Populus roots

Lectin receptor kinases in plant innate immunity

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