Proteomic profiling of Botrytis cinerea conidial germination

Abstract

Botrytis cinerea is one of the most relevant plant pathogenic fungi. The first step during its infection process is the germination of the conidia. Here, we report on the first proteome analysis during the germination of B. cinerea conidia, where 204 spots showed significant differences in their accumulation between ungerminated and germinated conidia by two-dimensional polyacrylamide gel electrophoresis and qPCR. The identified proteins were grouped by gene ontology revealing that the infective tools are mainly preformed inside the ungerminated conidia allowing a quick fungal development at the early stages of conidial germination. From 118 identified spots, several virulence factors have been identified while proteins, such as mannitol-1-phosphate dehydrogenase, 6,7-dimethyl-8-ribityllumazine synthase or uracil phosphoribosyltransferase, have been disclosed as a new potential virulence factors in botrytis whose role in pathogenicity needs to be studied to gain new insights about the role of these proteins as therapeutic targets and virulence factors.

Modifications of fungal membrane proteins profile under pathogenicity induction: A proteomic analysis ofBotrytis cinereamembranome

“…B. cinerea B05.10 strain (kindly provided by Dr. Paul Tudzunski from the University of Münster, Germany) was used for this study. Conidial stock suspension were prepared and maintained as previously reported . Two different carbon sources were included in our study: glucose (GLU) (Panreac, Spain) and TCW obtained from tomato fruits ( Lycopersicon esculentum cv.…”

Dataset of the Botrytis cinerea phosphoproteome induced by different plant-based elicitors

“…B. cinerea B05.10 strain (provided by Dr Paul Tudzynski from the University of Münster, Germany) was maintain in conidial stock suspension as previously reported in González-Rodriguez et al, 2015 [2] . Conidial suspension were inoculated to a final concentration of 5·10 4 conidia/mL in 250 mL of Minimal salt medium (50 mM NH4Cl, 7.3 mM KH2PO4, 4.2 mM MgSO4, 6.7 mM KCl, 0.07 mM FeSO4) supplemented with 1% of a sole carbon source: glucose (GLU) (Panreac, Spain) or tomato cell wall (TCW) obtained from tomato fruits ( Lycopersicon esculentum cv bola) of commercial maturity and deproteinized as previously reported [3] .…”

Proteomic Studies to Understand the Mechanisms of Peach Tissue Degradation by Monilinia laxa

“…The proteome has been used to determine proteins related to pathogenesis (El-Bebany et al, 2010;Ismail and Able, 2016) and in plant-based interactions (Shah et al, 2012). In this sense, a great effort has been made in the closely necrotrophic fungus B. cinerea to understand molecular mechanisms from a proteomic view, such as conidial germination (Espino et al, 2010;Gonzaĺez-Rodrıǵuez et al, 2014), modulation of protein secretion patterns under different carbon sources (Fernańdez-Acero et al, 2009;Shah et al, 2009b) and plant-based elicitors (Shah et al, 2009a;Fernańdez-Acero et al, 2010). Furthermore, how B. cinerea responded to non-nutritional changes such as pH (Li et al, 2012) and the involvement of membrane proteins in signal transduction cascades (Escobar-Niño et al, 2019).…”