Modifications of fungal membrane proteins profile under pathogenicity induction: A proteomic analysis ofBotrytis cinereamembranome

Abstract

Botrytis cinerea is a model fungus for the study of phytopathogenicity that exhibits a wide arsenal of tools to infect plant tissues. Most of these factors are related to signal transduction cascades, in which membrane proteins play a key role as a bridge between environment and intracellular molecular processes. This work describes the first description of the membranome of Botrytis under different pathogenicity conditions induced by different plant-based elicitors: glucose and tomato cell wall (TCW). A discovery proteomics analysis of membrane proteins was carried out by mass spectrometry. A total of 2794 proteins were successfully identified, 46% of them were classified as membrane proteins based on the presence of transmembrane regions and lipidation. Further analyses showed significant differences in the membranome composition depending on the available carbon source: 804 proteins were exclusively identified when the fungus was cultured with glucose as a sole carbon source, and 251 proteins were exclusively identified with TCW. Besides, among the 1737 common proteins, a subset of 898 proteins presented clear differences in their abundance. GO enrichment and clustering interaction analysis revealed changes in the composition of membranome with increase of signalling function in glucose conditions and carbohydrate degradation process in TCW conditions. All MS data have been deposited in the ProteomeXchange with identifier PXD003099 (http://proteomecentral.proteomexchange.org/dataset/PXD003099).

Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation

“…cinerea only cover around 10% of the total proteins predicted in the genome database 9 . In an attempt to increase this coverage, several different subproteomes have been characterized, particularly phosphoproteins 10,11 , and membrane proteins 12 . To further investigate the protein components of transduction pathways, a study has been undertaken to obtain the subproteome (phosphoproteins) of a subproteome (membrane proteins) with the object of revealing those membrane proteins that are controlled by phosphorylation.…”

“…Both conditions are known to induce different fungal responses in the B . cinerea phosphoproteome and membranome 11,12 . Interestingly, the differences identified in the composition of membrane-associated phosphoproteins in each assayed condition may indicate the relative importance of the regulation of particular factors - the pyruvate metabolism, unfolded protein response (UPR), oxidative stress response, autophagy and cell death - during the infection process of B .…”

Comparative quantitative proteomics of osmotic signal transduction mutants in Botrytis cinerea explain mutant phenotypes and highlight interaction with cAMP and Ca2+ signalling pathways

“…Although shotgun proteomics have in principle democratized global proteome analyses, such as subcellular proteomics, dynamic proteomic changes and posttranslational modification, the required technical equipment and know-how or access to proteomic platforms, as well as costs of analysis are still important limiting factors orientating functional genomics in favour of transcriptomics [27]. Proteomic analyses in Botrytis cinerea have mainly focused on sub-proteomes (e.g., secreted or membrane proteins) or phosphoproteomes [28][29][30][31][32][33][34][35][36][37].…”

“…We also noted the accumulation of endopolygalacturonase 1 BcPGA1 and a putative pectin methyltransferase. All these secreted proteins, generally induced by plant cell wall extracts, are involved in the modification and degradation of the plant wall and, consequently, in plant infection [31,[35][36][37].…”

Pathogenicity and Virulence Factors of Fusarium graminearum Including Factors Discovered Using Next Generation Sequencing Technologies and Proteomics

“…Various forms of proteomics are significant in studying plant-pathogen relations and other factors which include elicitors. An example of this is phosphoproteomics which has to some extent been studied in necrotrophic pathogens like B. cinerea [156][157][158], Septoria tritici [159]. These studies need to be extended to F. graminearum to deepen the understanding of its pathogenicity and virulence.…”