Proteomic profiling and identification in peritoneal fluid of children treated by peritoneal dialysis

Raaijmakers, Renske; Pluk, Wendy; Schröder, Cornelis H.; Gloerich, Jolein; Cornelissen, Elisabeth A.M.; Wessels, Hans; Willems, J. L.; Monnens, L.A.H.; Heuvel, L.P.W.J. van den

doi:10.1093/ndt/gfn212

Cited by 33 publications

(19 citation statements)

References 23 publications

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“…Isolation of vesicles is a possible way to enrich for low abundance, albeit clinically relevant proteins in the PDE. The total number of proteins observed in PDE-derived vesicles by proteomics in the present study was vastly greater than that reported in other studies using total protein extraction [13,16,18,31]. A complete list of the proteins identified can be accessible from the online database (http://sysbio.chula.ac.th/Database/PDEV/).…”

Section: Discussionmentioning

confidence: 60%

Multiple extracellular vesicle types in peritoneal dialysis effluent are prominent and contain known biomarkers

et al. 2017

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Peritoneal dialysis inevitability results in activation of inflammatory processes and its efficiency is highly variable between patients. An improved method to isolate biomarkers and study pathophysiological mechanisms in peritoneal dialysis effluent (PDE) is expected to be of much benefit for the development of this treatment approach and help with patient management. Extracellular vesicles (EVs) are released as part of normal cellular processes. Their proteome is expected to reflect both type and health of their cell of origin. Although there is a significant interest in using EVs for “liquid biopsies”, little is reported of their presence or composition in plentiful dialysis waste fluids, including peritoneal dialysis effluent (PDE). Here we determined the presence of EVs in PDE and subsequently characterized their proteome. EVs were first isolated from PDE using differential centrifugation, then a further enrichment using size exclusion chromatography (SEC) was performed. The presence of EVs was demonstrated using transmission electron microscopy, and their particle counts were investigated using nanoparticle tracking analysis and dynamic light scattering. Using tandem mass spectrometry, marker proteins from three types of EVs i.e. apoptotic bodies, ectosomes, and exosomes were identified. The proteomic results demonstrated that the isolation of EVs by differential centrifugation helped enrich for over 2,000 proteins normally masked by abundant proteins in PDE such as albumin and SEC markedly further improved the isolation of low abundant proteins. Gene ontology analysis of all identified proteins showed the marked enrichment of exosome and membrane-associated proteins. Over 3,700 proteins were identified in total, including many proteins with known roles in peritoneal pathophysiology. This study demonstrated the prominence of EVs in PDE and their potential value as a source of biomarkers for peritoneal dialysis patients.

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Section: Discussionmentioning

confidence: 60%

Multiple extracellular vesicle types in peritoneal dialysis effluent are prominent and contain known biomarkers

et al. 2017

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“…Proteomic tools, such as two-dimensional gel electrophoresis (2-DE) and mass spectrometry, have been widely applied in the study of body fluids, including cerebrospinal fluid [12], pleural and pericardial effusions [13][14][15], urine [16][17][18], and peritoneal dialysate [19][20][21]. For peritoneal fluid analysis, previous studies focus on proteome changes in endometriosis and ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

Impact of uremic environment on peritoneum: A proteomic view

Wang¹,

Lin²,

Chien³

et al. 2012

Journal of Proteomics

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“…Proteomic general analysis of peritoneal effluent has been already reported to give interesting results which can have useful application in the care of patients treated with PD [23][24][25]. However, in body fluid proteomics one of the main problems is represented by the very huge dynamic range of proteins, which can be as high as 10 10 or more in serum and plasma.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial Peptide Ligand Library and two dimensional electrophoresis: New frontiers in the study of peritoneal dialysis effluent in pediatric patients

Bruschi

Candiano

Santucci

et al. 2015

Journal of Proteomics

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Proteomic profiling and identification in peritoneal fluid of children treated by peritoneal dialysis

Abstract: This study gives a representative overview of the proteins present in PD fluid. The proteins in PD fluid reflect plasma proteins as well as local peritoneal processes. Potentially interesting proteins are revealed.

Cited by 33 publications

References 23 publications

Multiple extracellular vesicle types in peritoneal dialysis effluent are prominent and contain known biomarkers

Multiple extracellular vesicle types in peritoneal dialysis effluent are prominent and contain known biomarkers

Impact of uremic environment on peritoneum: A proteomic view

Combinatorial Peptide Ligand Library and two dimensional electrophoresis: New frontiers in the study of peritoneal dialysis effluent in pediatric patients

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