Impact of uremic environment on peritoneum: A proteomic view

Wang, Hsien Yi; Lin, Ching Yih; Chien, Chih Chiang; Kan, Wei; Tian, Yu; Liao, Pao Chi; Wu, Hung Tsung; Su, Shih Bin

doi:10.1016/j.jprot.2012.01.011

Cited by 12 publications

(7 citation statements)

References 62 publications

(57 reference statements)

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“…Several identified proteins in this study were reported as peritoneal dialysate proteins, such as APOA1, HP, ALB, IGHG1, and AZGP1 [15, 19]. In our study, some proteins were also indicated that may relate to DM.…”

Section: Resultssupporting

confidence: 58%

Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus

Yang

Wang

et al. 2013

BioMed Research International

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Peritoneal dialysis (PD) is an increasingly accepted modality of renal replacement therapy. It provides the advantages of having a flexible lifestyle, stable hemodynamics, and better preservation of residual renal function. To enhance our understanding of the peritoneal dialysate of diabetes mellitus (DM), peritoneal dialysate proteins were identified by two-dimensional gel electrophoresis (2DE) combined with reverse-phase nano-ultra performance liquid chromatography electrospray ionization tandem mass spectrometry (RP-nano-UPLC-ESI-MS/MS) followed by peptide fragmentation patterning. To validate the differential proteins, ELISA and Western blotting analyses were applied to detect candidate proteins that may be related to DM. We performed 2DE on the peritoneal dialysate samples, with detection of more than 300 spots. From this, 13 spots were excised, in-gel digested, and identified by RP-nano-UPLC-ESI-MS/MS. Ten of these showed significant differential expression between the DM and chronic glomerulonephritis (CGN) peritoneal dialysate samples. In this study, we conducted a comparative proteomic study on these two groups of dialysate that may provide evidence for understanding the different peritoneal protein changes. These proteins may not be new biomarkers; however, they may indicate a situation for possible drug treatment and can be the predictors of peritonitis for a validation study in the future.

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Section: Resultssupporting

confidence: 58%

Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus

Yang

Wang

et al. 2013

BioMed Research International

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“…At the time of PD catheter insertion, sub-mesothelial thickening and vasculopathy were observed in the peritoneum of uremic patients as compared to controls with normal renal function [ 7 ]. Wang et al [ 24 ] examined the proteome of peritoneal fluids collected from ESRD patients at the time of catheter insertion as well as from subjects with normal renal function undergoing selective laparoscopic cholecystectomy. A total of 16 spots representing 12 proteins had altered expression levels among the peritoneal fluids of the study groups.…”

Section: Proteomic Investigations In Peritoneal Dialysismentioning

confidence: 99%

Proteomic Research in Peritoneal Dialysis

Bonomini

Borràs

Troya-Saborido

et al. 2020

IJMS

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Peritoneal dialysis (PD) is an established home care, cost-effective renal replacement therapy (RRT), which offers several advantages over the most used dialysis modality, hemodialysis. Despite its potential benefits, however, PD is an under-prescribed method of treating uremic patients. Infectious complications (primarily peritonitis) and bio-incompatibility of PD solutions are the main contributors to PD drop-out, due to their potential for altering the functional and anatomical integrity of the peritoneal membrane. To improve the clinical outcome of PD, there is a need for biomarkers to identify patients at risk of PD-related complications and to guide personalized interventions. Several recent studies have shown that proteomic investigation may be a powerful tool in the prediction, early diagnosis, prognostic assessment, and therapeutic monitoring of patients on PD. Indeed, analysis of the proteome present in PD effluent has uncovered several proteins involved in inflammation and pro-fibrotic insult, in encapsulating peritoneal sclerosis, or even in detecting early changes before any measurable modifications occur in the traditional clinical parameters used to evaluate PD efficacy. We here review the proteomic studies conducted thus far, addressing the potential use of such omics methodology in identifying potential new biomarkers of the peritoneal membrane welfare in relation to dialytic prescription and adequacy.

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“…However, to date, the use of proteomics tools for the analysis of peritoneal fluid has only been reported in patients with uremia, endometriosis, ovarian cancer and following cases of peritoneal dialysis 26–29 , which has facilitated the development of biomarkers for these respective diseases/pathologies.…”

Section: Introductionmentioning

confidence: 99%

Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

Ruiz-Campillo

Molina-Hernández

Escamilla

et al. 2017

Sci Rep

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Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellular immune response, with infiltrating leukocytes and a marked eosinophilia (49%). However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated, these were not statistically different at 18 days post-infection compared to uninfected animals indicating that the immune response is muted and not yet skewed to a Th2 type response that is associated with chronic disease. Proteomic analysis of the peritoneal fluid identified infection-related proteins, including several structural proteins derived from the liver extracellular matrix, connective tissue and epithelium, and proteins related to the immune system. Periostin and vascular cell adhesion protein 1 (VCAM-1), molecules that mediate leukocyte infiltration and are associated with inflammatory disorders involving marked eosinophilia (e.g. asthma), were particularly elevated in the peritoneum. Immuno-histochemical studies indicated that the source of periostin and VCAM-1 was the inflamed sheep liver tissue. This study has revealed previously unknown aspects of the immunology and pathogenesis associated with acute fascioliasis in the peritoneum and liver.

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Impact of uremic environment on peritoneum: A proteomic view

Cited by 12 publications

References 62 publications

Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus

Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus

Proteomic Research in Peritoneal Dialysis

Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

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