Proteomic profile of the skin mucus of farmed gilthead seabream (Sparus aurata)

Jurado, Juan; Fuentes-Almagro, Carlos; Guardiola, Francisco A.; Prieto‐Álamo, María‐José

doi:10.1016/j.jprot.2015.02.019

Cited by 84 publications

(95 citation statements)

References 63 publications

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“…Despite the fact that this protein was previously identified in skin mucus of gilthead seabream [19], to our knowledge this is the first time that over-expression has been found after any probiotic diet, Pdp11 in this study. In addition, this protein has also reported in skin mucus of teleosts such as European sea bass [18] or Atlantic cod [34] but further studies are needed to clarify its implication in skin mucus.…”

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confidence: 52%

“…Structural proteins are a major group in seabream mucus proteins and include several isoforms of actins, keratins, tubulins, tropomyosin 4-2, cofilin-2 and filamin-A-like [19], and some of them are modulated in the present study (Tables 2 and 3). First, actin beta (ACTB; spots 7 and 21) was found differentially expressed in both experimental times (Table 3).…”

Section: Effects Of Pdp11 And/or Overcrowding Stress On Structural Prmentioning

confidence: 76%

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Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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Differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress, Journal of Proteomics (2015Proteomics ( ), doi: 10.1016Proteomics ( /j.jprot.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E DM A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractGilthead seabream (Sparus aurata L.) is the major cultured fish species in the Mediterranean area. High density stocking causes stress and increases the impact of diseases leading to economic losses. Probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. The probiotic Shewanella putrefaciens, also known as Pdp11, was firstly isolated from the skin of healthy gilthead seabream. Our study focuses on the skin mucus proteome after dietary probiotic Pdp11 intake in fish maintained under normal or overcrowding conditions. 2-DE of skin mucus followed by LC-MS/MS analysis was done for each experimental group and differentially expressed proteins were identified. The results showed differentially expressed proteins especially involved in immune processes, such as lysozyme, complement C3, natural killer cell enhancing factor and nonspecific cytotoxic cell receptor protein 1, whose transcript profiles were studied by qPCR. A consistency between lysozyme protein levels in the mucus and lysozyme mRNA levels in skin were found. Further research is necessary to unravel the implications of skin mucosal immunity on fish welfare and disease. Biological significanceThe present work reveals the proteomic changes, which are taking place in the skin mucus of stressed and non-stressed gilthead seabream after Pdp11 probiotic intake. The study contributes to improve the knowledge on skin mucosal immunology of this relevant farmed fish species.Furthermore, the paper shows for the first time how a suitable proteomic methodology, in this case 2-DE followed by LC-MS/MS is useful to perform a comparative study with a noninvasive technique of skin mucus of gilthead seabream.

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confidence: 52%

Section: Effects Of Pdp11 And/or Overcrowding Stress On Structural Prmentioning

confidence: 76%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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“…Valdenegro-Vega identified many different expressed proteins related to inflammation pathways in Atlantic salmon (Salmo salar) mucus after parasite challenge [8]. Jurado reported the first proteome map of the epidermal mucus of farmed gilthead seabream (Sparus aurata), and identified many bacterial peptides in the skin mucus related to fish innate immune response [9]. The knowledge of the immune responses in fish mucus kept growing, but most of the studies focused on enzyme activities and protein levels; no studies have characterized the gene expression profiles in fish mucus following bacterial challenge.…”

Section: Introductionmentioning

confidence: 99%

Expression profiling analysis of immune-related genes in channel catfish (Ictalurus punctatus) skin mucus following Flavobacterium columnare challenge

Ren

Zhao

et al. 2015

Fish & Shellfish Immunology

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“…Biofluid samples were collected from fish specimens using the method described by Jurado et al (2015) [15] with slight modifications. Briefly, the biofluid was collected by gently scrapping the skin surface of fish using plastic spatula.…”

Section: Preparation Of Fish Biofluidsmentioning

confidence: 99%

“…After fish died, Ncompounds chemical substances were also released comprised of amino acid, uric acid, purines, methylamine, taurine, imidazoles, creatine, and creatinine during postrigor stage [19]. Besides, the mucus mixed with blood and urinogenital or intestinal excretions once the fish died [15].…”

Section: Biofluid Metabolite Constituentsmentioning

confidence: 99%

Physicochemical Characterization of Biofluid Metabolites from Liquid Residual of Tuna Fish (Euthynnus affinis) throughout Refrigerated Storage Condition

Fazial

Ling

Ahmad

et al. 2017

Journal of Food Quality

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The cold storage condition and use of chemical treatment to preserve the fish appearance sometimes cause difficulties to the consumers to estimate the freshness of fish in the market. However, during fish deterioration, some compound is released or formed due to microbial and biochemical process. Identification of released compound during fish spoilage is a crucial step in understanding the degree of spoilage. This study characterizes the physicochemical changes of metabolites biofluids from liquid residual of tuna fish (Euthynnus affinis) during refrigerated storage condition. Tuna fish were kept in ice at 0 ∘ C and stored in cold room (∼4 ∘ C) for seven days in order to study the changes in fish freshness and loss of quality through the storage period. Liquid residual of fish was collected at 0, 1, 2, 3, 4, 5, 6, and 7 days of storage. LC-MS/MS analysis was carried out to determine the possible dominant compound which was later identified as creatine and phenylalanine. Quantification of creatine and phenylalanine using HPLC with UV detector found that creatine and phenylalanine increased significantly up to day 4 and day 5 upon storage time for creatine and phenylalanine, respectively ( < 0.05). The liquid residual pH increased from 6.5 on day 0 to 7.5 on day 7 ( < 0.05). Changes in chemical compounds were supported with physical analysis on gills colour of spoilage fish.* and * values decreased with storage time from 41.08 to 24.76 and 18.34 to 10.40, respectively, while * value increased from −3.80 to −0.46 ( < 0.05). The finding of biofluid derived compounds was found as useful and alternative indicators of fish freshness in later study on the development of optical biosensor.

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Proteomic profile of the skin mucus of farmed gilthead seabream (Sparus aurata)

Cited by 84 publications

References 63 publications

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Expression profiling analysis of immune-related genes in channel catfish (Ictalurus punctatus) skin mucus following Flavobacterium columnare challenge

Physicochemical Characterization of Biofluid Metabolites from Liquid Residual of Tuna Fish (Euthynnus affinis) throughout Refrigerated Storage Condition

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