Expression profiling analysis of immune-related genes in channel catfish (Ictalurus punctatus) skin mucus following Flavobacterium columnare challenge

Ren, Yichao; Zhao, Hongling; Su, Baofeng; Peatman, Eric; Li, Chao

doi:10.1016/j.fsi.2015.07.021

Cited by 27 publications

(12 citation statements)

References 54 publications

(61 reference statements)

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“…32−35 Transcriptional repression of immune-related genes also occurred in the epidermal mucus of channel catfish following bacterial exposure, which could have downstream effects on the susceptibility to pathogen infection. 15,32,36 In our study, downregulation of immune-related genes was predicted to inhibit the inflammatory response, leukocyte migration, activation of leukocytes, activation of phagocytes, T cell development, and antibacterial responses (Figure 2A). 33 Moreover, predicted alterations in immune function are consistent with proteomic studies of mucus in which the abundance and compositional profile of immune-related proteins are altered in response to biotic and abiotic stressors.…”

Section: Environmental Science and Technology Lettersmentioning

confidence: 62%

“…14 However, the role of RNA in the mucus remains unknown. Mucosal mRNA in channel catfish (Ictalurus punctatus) presented with a bacterial challenge showed differential expression of immune-related transcripts using quantitative polymerase chain reaction (qPCR), 15 suggesting transcriptional changes occur in mucus and can be used to assess the whole-animal stress response. Whole-transcriptome sequencing of mucus has yet to be performed and may provide insight into the potential use of mucus mRNA biomarkers.…”

Section: ■ Introductionmentioning

confidence: 99%

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Whole-Transcriptome Sequencing of Epidermal Mucus as a Novel Method for Oil Exposure Assessment in Juvenile Mahi-Mahi (Coryphaena hippurus)

Greer

Andrzejczyk

Mager

et al. 2019

Environ. Sci. Technol. Lett.

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Crude oil-derived polycyclic aromatic hydrocarbons (PAHs) are pervasive environmental pollutants with well-established detrimental effects on the health of marine organisms. Following large-scale oil spills in the marine environment, there is a critical need for noninvasive sampling methods to assess environmental exposure to PAHs without further perturbations to the population and for long-term monitoring following a spill. To test the efficacy of epidermal mucus mRNA as a source for noninvasive sampling, juvenile mahi-mahi (Coryphaena hippurus, ∼28 days of age) were exposed to control seawater or two concentrations of high-energy water accommodated fractions (HEWAFs; 5% or 10%) of Deepwater Horizon surface oil for 48 h. Whole-transcriptome sequencing revealed differential expression of 501 transcripts in the low-HEWAF exposure (∑PAH = 16.55 μg/L) and 196 transcripts in the high-HEWAF exposure (∑PAH = 23.03 μg/L), suggesting differential regulation of mRNA in mucus following PAH exposure. In addition to differential expression of well-established biomarkers of PAH exposure such as cytochrome P450 enzymes, the mucosal transcriptome showed differential expression of transcripts involved in immune response, cardiotoxicity, and calcium homeostasis that parallel molecular responses in whole embryos. The consistency of the changes in expression in the epidermal mucus compared to that of tissues obtained from lethal sampling suggests that mucus is a promising source for noninvasive monitoring techniques.

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Section: Environmental Science and Technology Lettersmentioning

confidence: 62%

Section: ■ Introductionmentioning

confidence: 99%

Whole-Transcriptome Sequencing of Epidermal Mucus as a Novel Method for Oil Exposure Assessment in Juvenile Mahi-Mahi (Coryphaena hippurus)

Greer

Andrzejczyk

Mager

et al. 2019

Environ. Sci. Technol. Lett.

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“…For instance, recent experiments in gilthead sea bream ( Sparus aurata L.) indicate that the gene expression pattern of gut mucins is altered by dietary oils and parasitic enteritis (Pérez-Sánchez et al, 2013b ). In addition to glycoproteins, glycosaminoglycans, immunoglobulins, lectins, pheromones, and proteolytic enzymes have been identified in the mucus of different fish species (Fletcher and Grant, 1969 ; Hjelmeland et al, 1983 ; van de Winkel et al, 1986 ; Shiomi et al, 1988 ; Shephard, 1994 ; Subramanian et al, 2008 ; Guardiola et al, 2014 ; Ren et al, 2015 ). Most of these molecules are involved in fish innate immunity and skin mucus is considered a key component of fish immune responses (Ellis, 2001 ; Salinas et al, 2011 ; Esteban, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Skin Mucus of Gilthead Sea Bream (Sparus aurata L.). Protein Mapping and Regulation in Chronically Stressed Fish

et al. 2017

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The skin mucus of gilthead sea bream was mapped by one-dimensional gel electrophoresis followed by liquid chromatography coupled to high resolution mass spectrometry using a quadrupole time-of-flight mass analyzer. More than 2,000 proteins were identified with a protein score filter of 30. The identified proteins were represented in 418 canonical pathways of the Ingenuity Pathway software. After filtering by canonical pathway overlapping, the retained proteins were clustered in three groups. The mitochondrial cluster contained 59 proteins related to oxidative phosphorylation and mitochondrial dysfunction. The second cluster contained 79 proteins related to antigen presentation and protein ubiquitination pathways. The third cluster contained 257 proteins where proteins related to protein synthesis, cellular assembly, and epithelial integrity were over-represented. The latter group also included acute phase response signaling. In parallel, two-dimensional gel electrophoresis methodology identified six proteins spots of different protein abundance when comparing unstressed fish with chronically stressed fish in an experimental model that mimicked daily farming activities. The major changes were associated with a higher abundance of cytokeratin 8 in the skin mucus proteome of stressed fish, which was confirmed by immunoblotting. Thus, the increased abundance of markers of skin epithelial turnover results in a promising indicator of chronic stress in fish.

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“…F. columnare is a prime example of a bacterial pathogen that is dependent on attachment to host mucosal surfaces to cause infection. Most studies on this focus primarily on the liver, spleen, and kidney immune factors; however, by examining the expression patterns within the mucus, researchers can determine whether attached bacteria suppress host immune responses [ 96 ]. The immune response of the mucosal surfaces of channel catfish was also investigated during A. hydrophila infections.…”

Section: Bacterial Co-infectionsmentioning

confidence: 99%

“…The immune response of the mucosal surfaces of channel catfish was also investigated during A. hydrophila infections. Vital lectins and proteins were observed to be altered, potentially enhancing the pathogen’s ability to disrupt and adhere to the mucosal barrier [ 96 ]. Though studies were conducted to determine single pathogen effects on the mucosal surface of catfish, similar studies were not conducted to document multiple pathogen effects.…”

Section: Bacterial Co-infectionsmentioning

confidence: 99%

A Review of Bacterial Co-Infections in Farmed Catfish: Components, Diagnostics, and Treatment Directions

Wise

LaFrentz

Kelly

et al. 2021

Animals

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Catfish production is a major aquaculture industry in the United States and is the largest sector of food fish production. As producers aim to optimize production yields, diseases caused by bacterial pathogens are responsible for high pond mortality rates and economic losses. The major bacterial pathogens responsible are Edwardsiella ictaluri, Aeromonas spp., and Flavobacterium columnare. Given the outdoor pond culture environments and ubiquitous nature of these aquatic pathogens, there have been many reports of co-infective bacterial infections within this aquaculture sector. Co-infections may be responsible for altering disease infection mechanics, increasing mortality rates, and creating difficulties for disease management plans. Furthermore, proper diagnoses of primary and secondary pathogens are essential in ensuring the correct treatment approaches for antimicrobials and chemical applications. A thorough understanding of the interactions and infectivity dynamics for these warm water bacterial pathogens will allow for the adoption of new prevention and control methods, particularly in vaccine development. This review aims to provide an overview of co-infective pathogens in catfish culture and present diagnostic case data from Mississippi and Alabama to define prevalence for these multiple-species infections better.

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Expression profiling analysis of immune-related genes in channel catfish (Ictalurus punctatus) skin mucus following Flavobacterium columnare challenge

Cited by 27 publications

References 54 publications

Whole-Transcriptome Sequencing of Epidermal Mucus as a Novel Method for Oil Exposure Assessment in Juvenile Mahi-Mahi (Coryphaena hippurus)

Whole-Transcriptome Sequencing of Epidermal Mucus as a Novel Method for Oil Exposure Assessment in Juvenile Mahi-Mahi (Coryphaena hippurus)

Skin Mucus of Gilthead Sea Bream (Sparus aurata L.). Protein Mapping and Regulation in Chronically Stressed Fish

A Review of Bacterial Co-Infections in Farmed Catfish: Components, Diagnostics, and Treatment Directions

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