Proteomic identification of the related immune-enhancing proteins in shrimp Litopenaeus vannamei stimulated with vitamin C and Chinese herbs

Qiao, Jie; Du, Zhiheng; Zhang, Yueling; Du, Hong; Guo, Lingling; Zhong, Min; Cao, Jingsong; Wang, Xiuying

doi:10.1016/j.fsi.2011.07.005

Cited by 63 publications

(34 citation statements)

References 37 publications

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“…probiotics from either commercial or government sources were used commonly in the study region. Many farmers used pineapple or banana as probiotics because they contain substantial amounts of vitamin C (Klimczak et al 2007), which has been shown to improve stress and non-specific defense responses in shrimp (Lee & Shiau 2002, Qiao et al 2011. Some lactic acid bacteria have been reported to enhance growth of shrimp and fish (Kesarcodi-Watson et al 2008, Lara-Flores 2011, Tuan et al 2013, Aguilera-Rivera et al 2014.…”

Section: Discussionmentioning

confidence: 99%

White spot disease risk factors associated with shrimp farming practices and geographical location in Chanthaburi province, Thailand

Piamsomboon

Inchaisri²

2015

Dis. Aquat. Org.

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Over the past 2 decades, shrimp aquaculture in Thailand has been impacted by white spot disease (WSD) caused by white spot syndrome virus (WSSV). Described here are results of a survey of 157 intensive shrimp farms in Chanthaburi province, Thailand, to identify potential farm management and location risk factors associated with the occurrence of WSD outbreaks. Logistic regression analysis of the survey responses identified WSD risks to be associated with farms sharing inlet water and culturing shrimp year round and with a single owner operating more than 1 farm. The analysis also showed WSD risks to be reduced at farms that used probiotics and applied lime to pond bottoms when fallow to neutralize acidity and kill microorganisms. Regression modeling identified no association of geographical location with WSD. The data should assist shrimp farms in mitigating the effects of WSD in Thailand.KEY WORDS: Epidemiology · Risk factors · Shrimp farming · Thailand · White spot disease · WSSV · Pacific white shrimp · Litopenaeus vannamei Resale or republication not permitted without written consent of the publisherDis Aquat Org 117: [145][146][147][148][149][150][151][152][153] 2015 sities, the feeding of live mollusks, farm sharing of water sources, and increased pond size to be associated with increased WSD risks (Tendencia et al. 2011). Other studies identified increased mangrove to pond area ratios, the use of vitamin dietary supplements, water filtration through 300 µm mesh screens, and pond bottom dry-out and decontamination, including plowing and sludge removal between crops, as factors that reduce WSD risks (Corsin et al. 2005, Tendencia et al. 2011.Risk analyses can be instructive as to what measures might assist in mitigating disease impacts in a particular farming region. The purpose of this study was thus to identify risk factors associated with the occurrence of WSD in Chanthaburi province, an intensive shrimp aquaculture region in Thailand, so that these might be used to guide government and farm WSD control policies. MATERIALS AND METHODS Study population and sizeThe study focused on Pacific white shrimp Litopenaeus vannamei farms located in the coastal area of Chanthaburi province, Thailand ( Fig. 1) and was conducted between October 2011 and December 2013 in collaboration with the Chanthaburi Coastal Fisheries Research and Development Centre (CFRD), Department of Fisheries. Chanthaburi province is among the largest shrimp aquaculture regions in Thailand, producing > 60 000 t yr −1 (Department of Fisheries 2014). Farmers generally purchase shrimp postlarvae age 10 to 12 d (PL10−PL12) from local or nearby (< 300 km) hatcheries for grow-out in earthen ponds for 90 to 120 d.The case−control study used a questionnaire to interview farmers (the full questionnaire is provided in the Supplement, available at www.int-res.com/ articles/ suppl_d117p145_supp.pdf). Farms were selected arbitrarily from 886 farms located in the CFRD study area. Farms were divided into WSD case and control groups based on the...

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Section: Discussionmentioning

confidence: 99%

White spot disease risk factors associated with shrimp farming practices and geographical location in Chanthaburi province, Thailand

Piamsomboon

Inchaisri²

2015

Dis. Aquat. Org.

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“…Hemocytes in the hemolymph are the major component of the cellular immune responses in crustaceans. In addition to it primarily being a respiratory protein, hemocyanin is a novel and valuable defense molecule in molluscs and arthropods (Zhang et al 2006(Zhang et al , 2009Qiao et al 2011;Cao et al 2014). In this study, hemocyanin was present in five altered protein spots (spots 2, 6, 7, 13, and 14).…”

Section: Discussionmentioning

confidence: 59%

“…Using the method described by Qiao et al (2011), 2-DE SDS PAGE was performed on the hemocyte proteins. The 2-DE gel images were analyzed using PDQuest software (version 8.0, Bio-Rad, Hercules, CA, USA).…”

Section: -De Sds Page Imaging Analysis and Ms Analysis For Hemocytmentioning

confidence: 99%

Immune Response of Mud Crab, Scylla Paramamosain, to Bacterial Lipopolysaccharide

Wang

You

et al. 2016

J World Aquaculture Soc

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Vibrio spp. is one of the main pathogens to cause high levels of mortality in the culture industry of mud crabs, Scylla paramamosain. Although considerable efforts have been devoted to understanding the immune response of S. paramamosain against pathogens, both immune‐response proteins and systematic responses to foreign agent invasions have yet to be investigated in detail. Hence, in this study, we challenged S. paramamosain with bacterial lipopolysaccharides (LPS) by injection. We examined a number of immune‐related indices in the crabs at 0, 2, 6, 12, 24, 36, 48, and 72 h postinjection, that is, the total hemocyte counts in hemolymph; the serum activities of phenoloxidase, superoxide dismutase, lysozyme, acid phosphatase, and alkaline phosphatase; and the mRNA levels of immune‐related genes encoding prophenoloxidase, serine protease inhibitors, antimicrobial peptide scygonadin, crustin, anti‐lipopolysaccharide factor, and catalase in hemocytes. In addition, the immune‐response proteins were identified in hemocytes at 24 h postinjection using two‐dimensional polyacrylamide gel electrophoresis. The results showed that the immune‐related enzymes and genes examined were induced in a time‐dependent manner post‐LPS challenge. Moreover, 18 altered proteins were present in the hemocytes and the main proteins to be altered were highly homologous to hemocyanin and protein kinase. Ultimately, this study provides useful information for understanding the immune response of S. paramamosain to bacterial infection.

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“…We performed 2-DE of the IgG fractions as described previously [25]. A total of 20 µg of IgG, h-IgG or hs-IgG in rehydration buffer (7 M urea, 2 M thiourea, 4% CHAPS, 0.2% DTT and 3.4 µl of immobilized pH gradient [IPG] buffer, pH 3–10) was used to rehydrate the IPG strip (7 cm, pH 7–10, Bio-Rad, USA) for 16 h. Isoelectric focusing (IEF) was performed at a constant temperature of 20°C using a continuous increase in voltage (up to 4000 V) for 65,000 Vh.…”

Section: Methodsmentioning

confidence: 99%

Characterization of a Novel Hemolytic Activity of Human IgG Fractions Arising from Diversity in Protein and Oligosaccharide Components

et al. 2014

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Human IgG is a well-established multifunctional antigen specific immunoglobulin molecule of the adaptive immune system. However, an antigen nonspecific immunological function of human IgG has never been reported. In this study, human IgG was isolated using ammonium sulfate fractional precipitation and diethylaminoethanol (DEAE) cellulose 52 ion exchange chromatography, from which h-IgG and hs-IgG fractions were purified on the basis of their differential binding to rabbit anti-shrimp hemocyanin antibody (h) and rabbit anti-shrimp hemocyanin's small subunit antibody (hs), respectively. We found that h-IgG had a higher hemolytic activity than hs-IgG against erythrocytes from humans, rabbits, mice and chickens, whereas the control IgG showed negligible activity. h-IgG could interact directly with erythrocyte membranes, and this interaction was suppressed by high molecular weight osmoprotectants, showing that it may follow a colloid-osmotic mechanism. In comparative proteomics and glycomics studies, h-IgG and hs-IgG yielded 20 and 5 significantly altered protein spots, respectively, on a 2-D gel. The mean carbohydrate content of h-IgG and hs-IgG was approximately 3.6- and 2-fold higher than that of IgG, respectively, and the α-d-mannose/α-d-glucose content was in the order of h-IgG>hs-IgG>IgG. In this study, a novel antigen nonspecific immune property of human IgG was investigated, and the diversity in the protein constituents and glycosylation levels may have functional signficance.

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Proteomic identification of the related immune-enhancing proteins in shrimp Litopenaeus vannamei stimulated with vitamin C and Chinese herbs

Cited by 63 publications

References 37 publications

White spot disease risk factors associated with shrimp farming practices and geographical location in Chanthaburi province, Thailand

White spot disease risk factors associated with shrimp farming practices and geographical location in Chanthaburi province, Thailand

Immune Response of Mud Crab, Scylla Paramamosain, to Bacterial Lipopolysaccharide

Characterization of a Novel Hemolytic Activity of Human IgG Fractions Arising from Diversity in Protein and Oligosaccharide Components

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