Proteomic identification of Galectin-11 and 14 ligands from Haemonchus contortus

Abstract

Haemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study,LGALS-11 and LGALS-14 ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry. AbstractHaemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death.The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study, ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry.

The interaction of host and nematode galectins influences the outcome of gastrointestinal nematode infections

“…It also damaged L4 during in vitro exposure (Sakthivel et al ., 2020). The targets of gal-11 and ovine gal-14 have been investigated by affinity chromatography combined with mass spectrometry and over 30 molecules from adult H. contortus have been identified for each galectin (Sakthivel et al ., 2018).…”