Proteomic evaluation of coffee zygotic embryos in two different stages of seed development

Franco, Octávio L.; Pelegrini, Patrícia B.; Gomes, Clarissa P.C.; Souza, Andréa Lúcia Teixeira de; Costa, Fábio T.; Domont, Gilberto B.; Quirino, Betânia Ferraz; Eira, M. T. S.; Mehta, Ângela

doi:10.1016/j.plaphy.2009.08.008

Cited by 14 publications

(5 citation statements)

References 40 publications

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“…Most of these spots ranged in mass from 22 to 66 kDa and in pI from 4.0 to 7.0. Proteomic analysis of later coffee seed maturation stages (210 and 255 DAA) showed a profile with approximately 185 spots, mostly with pI varying from 5.0 to 7.0 and mass from 10 to 115 kDa (Franco et al, 2009). The variation in the number of expressed proteins in the early and later fruit developmental stages reflects the characteristic metabolism of each phase.…”

Section: Comparative Analysis Of Proteomic Mapsmentioning

confidence: 97%

“…A study of the coffee seed development using proteomics and two-dimensional (2-D) gels was reported recently. It focused on zygotic embryos at two later developmental stages, 210 and 255 days after anthesis (DAA), since coffee seed development is accompanied by severe modifications in water-soluble proteins and several of these are associated with a specific developmental stage (Franco et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Comparative proteomic analysis between early developmental stages of the Coffea arabica fruits

Bandil¹,

Etto²,

Galvão³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Coffee is one of the most valuable agricultural commodities. There is much agronomic research on coffee, but molecular knowledge of its fruit development and ripening is limited. This study reports a comparative proteomic investigation of immature coffee fruits in two early developmental stages: stage 1, cell division and elongation of the perisperm; and stage 2, early growth of the endosperm progressively replacing the perisperm. Proteins were extracted using a modified SDSphenol method and two-dimensional electrophoresis gels stained with Coomassie blue revealed about 300 well-resolved polypeptide spots in the pH range of 3 to 10. The differentially expressed polypeptides spots were excised, trypsin-digested, and analyzed by MALDI-TOF mass spectrometry. Peptide MS data were searched against the coffee EST database. Most of the identified protein spots are involved in the glycolytic pathway and energy reserve, and are more highly expressed at stage 2.

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Section: Comparative Analysis Of Proteomic Mapsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Comparative proteomic analysis between early developmental stages of the Coffea arabica fruits

Bandil¹,

Etto²,

Galvão³

et al. 2013

Genet. Mol. Res.

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“…Proteins, peptides, and free amino acids are strongly related to beverage quality because they serve as precursors to aromas and flavors . A number of proteomic studies analyzing fruits from coffee cultivars in different maturation stages have identified proteins related to carbohydrate biosynthesis, peroxide reduction, RNA splicing, ATP synthesis, photosynthesis, defense and disease resistance, cold tolerance, and nitrogen source. − However, most of those studies were performed at the final stages of fruit maturation or with the whole fruit instead of isolated tissues.…”

Section: Introductionmentioning

confidence: 99%

Differentially Accumulated Proteins in Coffea arabica Seeds during Perisperm Tissue Development and Their Relationship to Coffee Grain Size

Alves

Magalhães

Labate

et al. 2016

J. Agric. Food Chem.

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Coffee is one of the most important crops for developing countries. Coffee classification for trading is related to several factors, including grain size. Larger grains have higher market value then smaller ones. Coffee grain size is determined by the development of the perisperm, a transient tissue with a highly active metabolism, which is replaced by the endosperm during seed development. In this study, a proteomics approach was used to identify differentially accumulated proteins during perisperm development in two genotypes with regular (IPR59) and large grain sizes (IPR59-Graudo) in three developmental stages. Twenty-four spots were identified by MALDI-TOF/TOF-MS, corresponding to 15 proteins. We grouped them into categories as follows: storage (11S), methionine metabolism, cell division and elongation, metabolic processes (mainly redox), and energy. Our data enabled us to show that perisperm metabolism in IPR59 occurs at a higher rate than in IPR59-Graudo, which is supported by the accumulation of energy and detoxification-related proteins. We hypothesized that grain and fruit size divergences between the two coffee genotypes may be due to the comparatively earlier triggering of seed development processes in IPR59. We also demonstrated for the first time that the 11S protein is accumulated in the coffee perisperm.

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“…A very efficient method involves the dissolution of proteins in phenol and precipitation in ammonium acetate in methanol (Hurkman and Tanaka, 1986). This method was successfully used for coffee (Franco et al, 2009), olive leaves (Wang et al, 2003), and other recalcitrant tissues such as those from tomato, avocado, and orange peel (Saravanan and Rose, 2004), as well as banana, apple, and potato (Carpentier et al, 2005). Despite the description of various biological properties of guarana and significant amounts of information that were obtained from its transcriptome, proteomic profiles from the different fruit tissues in the various developmental phases have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Method for obtaining high-resolution proteomic analysis from pericarps of guarana

Souza¹,

Angelo²,

Nogueira³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol. The extract obtained from the TCA/acetone precipitation presented low solubility and contamination with lipids and carbohydrates. On the Protein extraction from Paullinia cupana other hand, the quality of the extract gradually improved after using phenol and PVPP/phenol, enabling a yield up to 2 mg/g macerated tissues and the detection of 457 spots by 2-dimensional electrophoresis. The effectiveness of the procedure used was validated by identification of 10 randomly selected proteins by mass spectrometry. The procedure described here can be a starting point for applications using tissues of other organs of guarana or tissues of species that are similar to guarana.

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Proteomic evaluation of coffee zygotic embryos in two different stages of seed development

Cited by 14 publications

References 40 publications

Comparative proteomic analysis between early developmental stages of the Coffea arabica fruits

Comparative proteomic analysis between early developmental stages of the Coffea arabica fruits

Differentially Accumulated Proteins in Coffea arabica Seeds during Perisperm Tissue Development and Their Relationship to Coffee Grain Size

Method for obtaining high-resolution proteomic analysis from pericarps of guarana

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