Differentially Accumulated Proteins in Coffea arabica Seeds during Perisperm Tissue Development and Their Relationship to Coffee Grain Size

Abstract

Coffee is one of the most important crops for developing countries. Coffee classification for trading is related to several factors, including grain size. Larger grains have higher market value then smaller ones. Coffee grain size is determined by the development of the perisperm, a transient tissue with a highly active metabolism, which is replaced by the endosperm during seed development. In this study, a proteomics approach was used to identify differentially accumulated proteins during perisperm development in two genotypes with regular (IPR59) and large grain sizes (IPR59-Graudo) in three developmental stages. Twenty-four spots were identified by MALDI-TOF/TOF-MS, corresponding to 15 proteins. We grouped them into categories as follows: storage (11S), methionine metabolism, cell division and elongation, metabolic processes (mainly redox), and energy. Our data enabled us to show that perisperm metabolism in IPR59 occurs at a higher rate than in IPR59-Graudo, which is supported by the accumulation of energy and detoxification-related proteins. We hypothesized that grain and fruit size divergences between the two coffee genotypes may be due to the comparatively earlier triggering of seed development processes in IPR59. We also demonstrated for the first time that the 11S protein is accumulated in the coffee perisperm.

Effect of Microwave Vacuum Drying on the Drying Characteristics, Color, Microstructure, and Antioxidant Activity of Green Coffee Beans

“…Coffee is one of the most popular beverages consumed by people around the world. Furthermore, it is the most important agricultural product whose production brings considerable economic benefits to certain developing tropical countries [ 1 ]. The two main commercial species of coffee are Coffea canephora and Coffea arabica , which account for 40% and 60%, respectively, of commercial coffee.…”

Seed tissue and nutrient partitioning, a case for the nucellus

“…A retard in the elimination of the nucellus is a hallmark of coffee grains. In coffee, the nucellus grows to define seed size and is then replaced by the endosperm (Alves et al 2016; Mayne 1937). Similarly, the nucellus of Austrobaileya scandens seeds drives early seed growth and is then eliminated by the endosperm, whose further development determines final seed size (Losada et al 2017).…”

Effect of the Post-Harvest Processing on Protein Modification in Green Coffee Beans by Phenolic Compounds

“…Coffee belongs to the global mainstream drinks [ 1 , 2 ]. The demand for coffee beverages is growing, and only crude oil has a larger trade volume [ 3 ].…”