Proteomic detection of changes in protein synthesis induced by lanthanum in BGC-823 human gastric cancer cells

Shi, Ping; Huang, Zhiwei

doi:10.1007/s10534-004-1812-9

Cited by 21 publications

(23 citation statements)

References 15 publications

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“…Lanthanum, a light rare earth element, has potential applications in medicine [22] and lanthanum carbonate is used in the treatment of hyperphosphatemia [23, 24]. Rare earth elements are reported to possess a number of physiological effects such as immune regulation [25, 26], anti-virus [27], and anticancer [28-30]. …”

Section: Introductionmentioning

confidence: 99%

Lanthanum Chloride Inhibits LPS Mediated Expressions of Pro-Inflammatory Cytokines and Adhesion Molecules in HUVECs: Involvement of NF-κB-Jmjd3 Signaling

Chen

Xiao

Xing

et al. 2017

Cell Physiol Biochem

4,206

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Background/Aims: To investigate the regulation of LaCl₃ on lipopolysaccharides (LPS)-induced pro-inflammatory cytokines and adhesion molecules in human umbilical vein endothelial cells (HUVECs). Methods: Primary cultured HUVECs were pretreated with 2.5 µM LaCl₃ for 30 min followed by 1 µg/ml LPS for 2 h. Pro-inflammatory cytokine and adhesion molecule expressions were determined by real-time RT-PCR and ELISA. NF-κB/p65 nuclear translocation was examined by immunofluorescence and immuno-blot, and its DNA-binding activity was measured by chemiluminescence. Recruitment of NF-κB/p65, Jmjd3, and H3K27me3 to gene promoter regions was determined by ChIP-qPCR. Results: LaCl₃ exhibited no cytotoxic effects to primary HUVECs at concentrations ≤ 50 µM. LPS-mediated TNF-α, IL-1β, IL-6, MMP-9, and ICAM-1 production, nuclear translocation, and DNA-binding activity of NF-κB/p65, as well as Jmjd3 expression, were all reduced significantly by LaCl₃. Furthermore, LaCl₃ treatment significantly impaired LPS-induced enrichment of NF-κB/p65 to the promoter regions of TNF-α, MMP-9, IL-1β, ICAM-1, and IL-6; and of Jmjd3 to the promoter regions of TNF-α, MMP-9, IL-1β, and IL-6. H3K27me3 abundance in the promoter regions of TNF-α and ICAM-1 increased significantly in following LaCl₃ treatment. Conclusion: LaCl₃ inhibits pro-inflammatory cytokine and adhesion molecule expressions induced by LPS in HUVECs. NF-κB and histone demethylase Jmjd3 are involved in this effect.

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Section: Introductionmentioning

confidence: 99%

Lanthanum Chloride Inhibits LPS Mediated Expressions of Pro-Inflammatory Cytokines and Adhesion Molecules in HUVECs: Involvement of NF-κB-Jmjd3 Signaling

Chen

Xiao

Xing

et al. 2017

Cell Physiol Biochem

4,206

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“…Therefore, La 3? -modulated cell proliferation or apoptosis in vitro may be derived from its effect on the inhibition of calcium fluxes which are required for cell cycle regulation (Dai et al 2002;Heffeter et al 2006;Sato et al 1998;Shi and Huang 2005).…”

Section: Introductionmentioning

confidence: 99%

Lanthanum chloride suppresses hydrogen peroxide-enhanced calcification in rat calcifying vascular cells

et al. 2008

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Lanthanum chloride (LaCl(3)) has been shown to retard the progression of established atherosclerotic lesions in animal models, and used as a calcium channel blocker in various cellular experiments. In this study, we assessed the role of lanthanum chloride (LaCl(3)) in H(2)O(2)-enhanced calcification in rat calcifying vascular cells (CVCs) and examined the involvement of MAPK signaling pathways. H(2)O(2) induced growth inhibition of CVCs, as well as increases in intracellular levels of calcium and reactive oxygen species, ALP activity, apoptosis and calcium deposition. These effects of H(2)O(2) were suppressed by pretreatment of the cells with 1 muM of LaCl(3) for 2 h. In addition, H(2)O(2) activated the phosphorylation of ERK1/2, JNK and p38 MAPK, but only the last two were associated with the ALP activity. Our findings demonstrate that H(2)O(2)-enhanced osteoblastic differentiation and apoptosis are responsible for the increased calcification in rat CVCs, and LaCl(3) can counteract these effects by suppressing the activation of JNK (JNK2, but not JNK1) and p38 MAPK signaling pathway.

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“…This element has applications in agriculture, industry and medicine (Palasz and Czekaj 2000). There is an increasing interest in its medical use including the therapeutic application of La as an anticancer agent, proposed by Shi and Huang (2005). In the last 25 years, new technologies in metallurgical, optical, and electronic industries increased the application of artificial lanthanide compounds with special physicochemical properties.…”

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confidence: 99%

Effects of Lanthanum on Human Lymphocytes Viability and DNA Strand Break

Paiva

Oliveira

Yunes

et al. 2008

Bull Environ Contam Toxicol

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Lanthanum (La) is a rare-earth metal with applications in agriculture, industry, and medicine. Since lanthanides show a broad spectrum of applications there is an increased risk of contamination for humans. We examined the effects of lanthanum in Jurkat cells and human peripheral lymphocytes (HPL), and we found that it was cytotoxic and genotoxic on both cell lines. Additionally, HPL were more sensitive to La treatment than Jurkat cells and necrosis was the pathway by which La induced cytotoxicity. Vitamin E was able to diminish the DNA strand breaks induced suggesting that oxidative stress may be involved in the genotoxic process.

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Proteomic detection of changes in protein synthesis induced by lanthanum in BGC-823 human gastric cancer cells

Cited by 21 publications

References 15 publications

Lanthanum Chloride Inhibits LPS Mediated Expressions of Pro-Inflammatory Cytokines and Adhesion Molecules in HUVECs: Involvement of NF-κB-Jmjd3 Signaling

Lanthanum Chloride Inhibits LPS Mediated Expressions of Pro-Inflammatory Cytokines and Adhesion Molecules in HUVECs: Involvement of NF-κB-Jmjd3 Signaling

Lanthanum chloride suppresses hydrogen peroxide-enhanced calcification in rat calcifying vascular cells

Effects of Lanthanum on Human Lymphocytes Viability and DNA Strand Break

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