Proteomic and Transcriptomic Analyses of Agave americana in Response to Heat Stress

Shakeel, Samina N.; Aman, Sitwat; Haq, Noor ul; Heckathorn, Scott A.; Luthe, Dawn S.

doi:10.1007/s11105-013-0555-6

Cited by 24 publications

(22 citation statements)

References 59 publications

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“…Finally, a limited correlation between protein expression and transcript expression existed in our results. This phenomenon also existed in Arabidopsis thaliana (Ng, Zhang et al., ), B. napus (Marmagne, Brabant, Thiellement, & Alix, ), Agave Americana (Shakeel, Aman, Ul Haq, Heckathorn, & Luthe, ), and so on, showing a wide spread low correlation between mRNA expression and protein expression. The lack of concordance between mRNA and protein abundance could lead to the condition that alterations on gene expression mediated by lncRNAs had no obvious influence on the protein abundance.…”

Section: Discussionmentioning

confidence: 91%

“…Americana (Shakeel, Aman, Ul Haq, Heckathorn, & Luthe, 2013), and so on, showing a wide spread low correlation between mRNA expression and protein expression. The lack of concordance between mRNA and protein abundance could lead to the condition that alterations on gene expression mediated by lncRNAs had no obvious influence on the protein abundance.…”

Section: Long-coding Rnas Play An Important Role In Gene Expressionmentioning

confidence: 99%

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Integrative analysis of genome‐wide lncRNA and mRNA expression in newly synthesized Brassica hexaploids

Wang

Zou

Meng

et al. 2018

Ecology and Evolution

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Polyploidization, as a significant evolution force, has been considered to facilitate plant diversity. The expression levels of lncRNAs and how they control the expression of protein‐coding genes in allopolyploids remain largely unknown. In this study, lncRNA expression profiles were compared between Brassica hexaploid and its parents using a high‐throughput sequencing approach. A total of 2,725, 1,672, and 2,810 lncRNAs were discovered in Brassica rapa, Brassica carinata, and Brassica hexaploid, respectively. It was also discovered that 725 lncRNAs were differentially expressed between Brassica hexaploid and its parents, and 379 lncRNAs were nonadditively expressed in this hexaploid. LncRNAs have multiple expression patterns between Brassica hexaploid and its parents and show paternal parent‐biased expression. These lncRNAs were found to implement regulatory functions directly in the long‐chain form, and acted as precursors or targets of miRNAs. According to the prediction of the targets of differentially expressed lncRNAs, 109 lncRNAs were annotated, and their target genes were involved in the metabolic process, pigmentation, reproduction, exposure to stimulus, biological regulation, and so on. Compared with the paternal parent, differentially expressed lncRNAs between Brassica hexaploid and its maternal parent participated in more regulation pathways. Additionally, 61 lncRNAs were identified as putative targets of known miRNAs, and 15 other lncRNAs worked as precursors of miRNAs. Some conservative motifs of lncRNAs from different groups were detected, which indicated that these motifs could be responsible for their regulatory roles. Our findings may provide a reference for the further study of the function and action mechanisms of lncRNAs during plant evolution.

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Section: Discussionmentioning

confidence: 91%

Section: Long-coding Rnas Play An Important Role In Gene Expressionmentioning

confidence: 99%

Integrative analysis of genome‐wide lncRNA and mRNA expression in newly synthesized Brassica hexaploids

Wang

Zou

Meng

et al. 2018

Ecology and Evolution

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“…Changes in protein accumulation under stress are directly related to the physiological phenotypic responses of plants to stress. The proteomic responses to high-temperature or flood stresses are known for several plants, including rice (Lin et al, 2005), wheat (Majoul et al, 2004), barley (Süle et al, 2004), soybean (Komatsu et al, 2013), radish (Zhang et al, 2013), Arabidopsis thaliana (Palmblad et al, 2008), Agave americana (Shakeel et al, 2013), and Populus euphratica (Ferreira et al, 2006). In the present study, we analyzed physiological and proteomics data in different genotypes of cauliflower under conditions of high-temperature and waterlogging stresses over different time periods.…”

Section: Biological or Biochemical Functions Of Flood-and Heat-responmentioning

confidence: 99%

Comparative proteomic analysis of cauliflower under high temperature and flooding stresses

Lin

Chen

et al. 2015

Scientia Horticulturae

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a b s t r a c tHigh-temperature and waterlogging are major abiotic stresses that affect the yield and quality of cauliflower. Cauliflower cultivars 'H41' and 'H69' are tolerant to high temperature and flooding, respectively; however, 'H71' is sensitive to both stresses. The objectives of this study were to identify the proteins that were differentially regulated and the physiological changes that occurred during different time periods in 'H41', 'H69', and 'H71' when responding to treatments of flooding, 40 • C, and both stresses combined. Changes in the leaf proteome were analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and identified by Mascot peptide mass fingerprint (PMF) and database searching. Stress treatments caused significant reductions in electrolyte leakage, chlorophyll fluorescence Fv/Fm, chlorophyll content, and water potential as stress times were prolonged. By the comparative proteomic analysis, 85 protein peaks that were differentially expressed in response to combination treatments at 0, 6, and 24 h, 69 (33 in 'H41', 29 in 'H69', and 9 in 'H71') were identified, of which were cultivar specific. Differentially regulated proteins predominantly functioned in photosynthesis and to a lesser extent in energy metabolism, cellular homeostasis, transcription and translation, signal transduction, and protein biosynthesis. This is the first report that utilizes proteomics to discover changes in the protein expression profile of cauliflower in response to heat and flooding.

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“…Different types of tissues are studied: roots [141,154,159], root nodules [161], leaves [138,160], roots and leaves compared at seedling stage [157], roots, flag leaves and spikelets at reproductive stage [151]. As the high temperature is particularly detrimental during the reproductive stage, besides the studies on leaf proteome under heat stress in rice [113,162], wheat [163] radish [142], alfalfa [164] and stromal proteins in agave [165], special attention is paid to the reproductive phase [166], source-sink interactions at grain filling [167], grain development and composition [155,160] in cereals, and protein composition of soybean seeds [168] formed under unfavorable temperature conditions. Relatively few proteomic studies deal with combined drought and heat stress [169][170][171].…”

Section: Proteomics In Search Of Molecular Markers For Assisted Selecmentioning

confidence: 99%

Selection and Breeding of Suitable Crop Genotypes for Drought and Heat Periods in a Changing Climate: Which Morphological and Physiological Properties Should Be Considered?

2016

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Selection and breeding of genotypes with improved drought/heat tolerance become key issues in the course of global change with predicted increased frequency of droughts or heat waves. Several morphological and physiological plant traits must be considered. Rooting depth, root branching, nutrient acquisition, mycorrhization, nodulation in legumes and the release of nutrients, assimilates or phytohormones to the shoot are relevant in root systems. Xylem embolism and its repair after a drought, development of axillary buds and solute channeling via xylem (acropetal) and phloem (basipetal and acropetal) are key processes in the stem. The photosynthetically active biomass depends on leaf expansion and senescence. Cuticle thickness and properties, epicuticular waxes, stomatal regulation including responses to phytohormones, stomatal plugs and mesophyll resistance are involved in optimizing leaf water relations. Aquaporins, dehydrins, enzymes involved in the metabolism of compatible solutes (e.g., proline) and Rubisco activase are examples for proteins involved in heat or drought susceptibility. Assimilate redistribution from leaves to maturing fruits via the phloem influences yield quantity and quality. Proteomic analyses allow a deeper insight into the network of stress responses and may serve as a basis to identify suitable genotypes, although improved stress tolerance will have its price (often lowered productivity under optimal conditions).

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Proteomic and Transcriptomic Analyses of Agave americana in Response to Heat Stress

Cited by 24 publications

References 59 publications

Integrative analysis of genome‐wide lncRNA and mRNA expression in newly synthesized Brassica hexaploids

Integrative analysis of genome‐wide lncRNA and mRNA expression in newly synthesized Brassica hexaploids

Comparative proteomic analysis of cauliflower under high temperature and flooding stresses

Selection and Breeding of Suitable Crop Genotypes for Drought and Heat Periods in a Changing Climate: Which Morphological and Physiological Properties Should Be Considered?

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