Comparative proteomic analysis of cauliflower under high temperature and flooding stresses

Abstract

a b s t r a c tHigh-temperature and waterlogging are major abiotic stresses that affect the yield and quality of cauliflower. Cauliflower cultivars 'H41' and 'H69' are tolerant to high temperature and flooding, respectively; however, 'H71' is sensitive to both stresses. The objectives of this study were to identify the proteins that were differentially regulated and the physiological changes that occurred during different time periods in 'H41', 'H69', and 'H71' when responding to treatments of flooding, 40 • C, and both stresses combined. Changes in the leaf proteome were analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and identified by Mascot peptide mass fingerprint (PMF) and database searching. Stress treatments caused significant reductions in electrolyte leakage, chlorophyll fluorescence Fv/Fm, chlorophyll content, and water potential as stress times were prolonged. By the comparative proteomic analysis, 85 protein peaks that were differentially expressed in response to combination treatments at 0, 6, and 24 h, 69 (33 in 'H41', 29 in 'H69', and 9 in 'H71') were identified, of which were cultivar specific. Differentially regulated proteins predominantly functioned in photosynthesis and to a lesser extent in energy metabolism, cellular homeostasis, transcription and translation, signal transduction, and protein biosynthesis. This is the first report that utilizes proteomics to discover changes in the protein expression profile of cauliflower in response to heat and flooding.

Proteomic Analysis Reveals the Positive Effect of Exogenous Spermidine in Tomato Seedlings' Response to High-Temperature Stress

“…Importantly, Rubisco and Rubisco activase (RCA) are the primary limiting factors of net photosynthesis under stress (Ahsan et al, 2007 ; Hu et al, 2015 ). In this study, we found that proteins related to Rubisco (spots 6, 28, 49) and RCA (spots 16, 64) markedly decreased in response to high-temperature stress, similar to other proteomic studies (Han et al, 2009 ; Lin K. H. et al, 2015 ). High temperature can reduce the activation state of Rubisco (Law and Crafts-Brandner, 1999 ), which is often attributed to the thermolability and loss of activity of RCA under high-temperature stress (Salvucci and Crafts-Brandner, 2004 ; Sharkey, 2005 ).…”

Respon Kembang Kol Dataran Rendah Terhadap Kepekatan Nutrisi pada Floating Hydroponic System Termodifikasi

“…Setiap varietas memiliki ketahanan yang berbeda terhadap keadaan lingkungan. Temperatur yang tinggi menyebabkan kembang kol membentuk krop yang tidak rata, tidak kompak, berwarna kuning dan muncul daun pada krop, daun tanaman sempit, pertumbuhan daun berkurang, inisiasi krop yang tertunda, yang semuanya menurunkan kualitas atau bahkan membuat krop tidak bisa dijual (Lin et al 2015). Varietas Mona memiliki hasil berat krop bunga tertinggi daripada varietas lain dengan rata-rata 104,84 g tiap tanaman (Gambar 2).…”

Analysis of Brassica oleracea early stage abiotic stress responses reveals tolerance in multiple crop types and for multiple sources of stress

“…Genechip assays have been used to compare expression patterns to differentiate between heat‐tolerant and heat‐susceptible cabbage lines, showing that heat‐tolerant lines show increased expression of heat shock proteins . Differential protein expression has also been noted in heat‐tolerant and heat‐sensitive cauliflower lines . Differential protein expression could also be responsible for variation in tolerance of other stresses, but is probably combined with morphological characteristics to determine the ultimate response of a particular line to each of the abiotic stresses.…”

“…3 Differential protein expression has also been noted in heat-tolerant and heat-sensitive cauliflower lines. 11 Differential protein expression could also be responsible for variation in tolerance of other stresses, but is probably combined with morphological characteristics to determine the wileyonlinelibrary.com/jsfa ultimate response of a particular line to each of the abiotic stresses. Assaying protein expression could be used to screen genetically fixed lines, such as those investigated in this study, for stress tolerance and to provide more detailed analysis and to determine mature plant responses.…”

“…In Chinese kale, both water deficit and waterlogging led to reduced leaf area, fresh and dry weight and leaf number, with drought leading to darker leaves and closed stomata . Short periods of waterlogging stress led to cultivar differences in cauliflower and broccoli in variables including heading percentage, yield per unit area, root dry weight, protein expression and growth scores . High salinity has been found to decrease shoot and root length and dry and fresh weight in cauliflower, broccoli, kale and cabbage…”