Proteomic analysis reveals oxidative stress response as the main adaptative physiological mechanism in cows under different production systems

Marco-Ramell, Anna; Arroyo, Laura; Saco, Yolanda; García-Heredia, Anabel; Camps, Jordi; Fina, Marta; Piedrafita, J.; Bassols, Anna

doi:10.1016/j.jprot.2012.04.002

Cited by 34 publications

(17 citation statements)

References 46 publications

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“…The carbonyl protein content was detected by slot blot and serum apolipoprotein A-I (Apo A-I) by immunoblotting, as described by Marco-Ramell et al [15, 16], respectively. A control sample was used in both immunoassays to compare results from different membranes.…”

Section: Methodsmentioning

confidence: 99%

“…Fifty μg protein was labelled with Cy2, Cy3 or Cy5 dyes (GE Healthcare, Buckinghamshire, UK) and subjected to a bidimensional electrophoresis on four IPG strips (pH 4–7, 24 cm, GE Healthcare) followed by 12.5% SDS-PAGE, as described in [16]. The pooled D1 sample was labelled with Cy2 and used as internal standard in all four gels.…”

Section: Methodsmentioning

confidence: 99%

“…A positive identification criterion was set as an individual Mascot score for each peptide MS/MS spectrum higher than the corresponding homology threshold score. Full details about the procedures are described in [16]. …”

Section: Methodsmentioning

confidence: 99%

“…Pooled samples were enriched with ProteoMiner™ (Bio-Rad, Hercules, CA) as described in [16] and then desalted and quantified. ProteoMiner was used to decrease the complexity of serum proteome since iTRAQ requires relatively simple protein mixtures [17].…”

Section: Methodsmentioning

confidence: 99%

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Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers

et al. 2016

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BackgroundThe objective assessment of animal stress and welfare requires proper laboratory biomarkers. In this work, we have analyzed the changes in serum composition in gilts after switching their housing, from pen to individual stalls, which is generally accepted to cause animal discomfort.ResultsBlood and saliva samples were collected a day before and up to four days after changing the housing system. Biochemical analyses showed adaptive changes in lipid and protein metabolism after the housing switch, whereas cortisol and muscular markers showed a large variability between animals. 2D-DIGE and iTRAQ proteomic approaches revealed variations in serum protein composition after changing housing and diet of gilts. Both techniques showed alterations in two main homeostatic mechanisms: the innate immune and redox systems. The acute phase proteins haptoglobin, apolipoprotein A-I and α1-antichymotrypsin 3, and the antioxidant enzyme peroxiredoxin 2 were found differentially expressed by 2D-DIGE. Other proteins related to the innate immune system, including lactotransferrin, protegrin 3 and galectin 1 were also identified by iTRAQ, as well as oxidative stress enzymes such as peroxiredoxin 2 and glutathione peroxidase 3. Proteomics also revealed the decrease of apolipoproteins, and the presence of intracellular proteins in serum, which may indicate physical injury to tissues.ConclusionsHousing of gilts in individual stalls and diet change increase lipid and protein catabolism, oxidative stress, activate the innate immune system and cause a certain degree of tissue damage. We propose that valuable assays for stress assessment in gilts may be based on a score composed by a combination of salivary cortisol, lipid metabolites, innate immunity and oxidative stress markers and intracellular proteins.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0887-1) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers

et al. 2016

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“…Свиньи, содержащиеся в усло-виях низкой и высокой плотности, также показали различия в общем холестерине, холестерине липопро-теинов низкой плотности и в уровнях свиного основ-ного белка острой фазы. Позже Marco-Ramell et al [26] идентифицировали глутатион пероксидазу, гли-копротеин α2 Heremans Schmid, холестерин и фекаль-ный кортикостерон как потенциальные биомаркеры bility to apply a proteomic approach to enable identification of meat species is under investigations, but data is still limited. Von Bargen et al [20] developed sensitive multiple reaction monitoring (MRM) mass spectrometry method which allowed to detect low concentrations of pork and horse meat in halal beef samples.…”

Section: протеомика в исследовании благополучия животныхunclassified

Proteomics in Meat Science — Current Status and Future Perspective

Zamaratskaia

2017

Teor. prakt. pererab. mâsa (Print)

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Concentration and pattern changes of porcine serum apolipoprotein A‐I in four different infectious diseases

et al. 2015

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Apolipoprotein A-I (Apo A-I) is a major protein in lipid/lipoprotein metabolism and decreased serum levels have been observed in many species in response to inflammatory and infectious challenges. Little is known about the porcine homologue, therefore in this work we have characterized it through biochemical and proteomic techniques. In 2DE, porcine serum Apo A-I is found as three spots, the two more acidic ones corresponding to the mature protein, the more basic spot to the protein precursor. Despite high sequence coverage in LC-MS/MS, we did not find a sequence or PTM difference between the two mature protein species. Besides this biochemical characterization, we measured overall levels and relative species abundance of serum Apo A-I in four different viral and bacterial porcine infectious diseases. Lower overall amounts of Apo A-I were observed in Salmonella typhimurium and Escherichia coli infections. In the 2DE protein pattern, an increase of the protein precursor together with a lower level of mature protein species were detected in the porcine circovirus type 2-systemic disease and S. typhimurium infection. These results reveal that both the porcine serum Apo A-I concentration and the species pattern are influenced by the nature of the infectious disease.

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Proteomic analysis reveals oxidative stress response as the main adaptative physiological mechanism in cows under different production systems

Cited by 34 publications

References 46 publications

Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers

Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers

Proteomics in Meat Science — Current Status and Future Perspective

Concentration and pattern changes of porcine serum apolipoprotein A‐I in four different infectious diseases

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