Concentration and pattern changes of porcine serum apolipoprotein A‐I in four different infectious diseases

Abstract

Apolipoprotein A-I (Apo A-I) is a major protein in lipid/lipoprotein metabolism and decreased serum levels have been observed in many species in response to inflammatory and infectious challenges. Little is known about the porcine homologue, therefore in this work we have characterized it through biochemical and proteomic techniques. In 2DE, porcine serum Apo A-I is found as three spots, the two more acidic ones corresponding to the mature protein, the more basic spot to the protein precursor. Despite high sequence coverage in LC-MS/MS, we did not find a sequence or PTM difference between the two mature protein species. Besides this biochemical characterization, we measured overall levels and relative species abundance of serum Apo A-I in four different viral and bacterial porcine infectious diseases. Lower overall amounts of Apo A-I were observed in Salmonella typhimurium and Escherichia coli infections. In the 2DE protein pattern, an increase of the protein precursor together with a lower level of mature protein species were detected in the porcine circovirus type 2-systemic disease and S. typhimurium infection. These results reveal that both the porcine serum Apo A-I concentration and the species pattern are influenced by the nature of the infectious disease.

Growth promotion in pigs by oxytetracycline coincides with down regulation of serum inflammatory parameters and of hibernation‐associated protein HP‐27

“…Sample preparation for mass spectrometry was performed as recently described . Briefly, spots of interest were washed, destained, reduced and alkylated before in‐gel tryptic digestion (TrypsinGold, Mass Spectrometry Grade, Promega, Madison, WI, USA).…”

Identification of Candidate Genes and Regulatory Competitive Endogenous RNA (ceRNA) Networks Underlying Intramuscular Fat Content in Yorkshire Pigs with Extreme Fat Deposition Phenotypes

“…Moreover, the target genes of other DELs ( Supplementary File S8 ), including ENSSSCT00000066793, ENSSSCT00000069743, ENSSSCT00000077515, MSTRG.14478.1, ENSSSCT00000080216, MSTRG.1567.1, MSTRG.4080.9 (target gene: ACACA ), ENSSSCT00000074683, ENSSSCT00000083654, ENSSSCT00000084825, ENSSSCT00000085428, ENSSSCT00000085972, ENSSSCT00000089741, ENSSSCT00000089741, MSTRG.14434.8, MSTRG.14355.1 (target gene: LEP ), ENSSSCT00000066499, MSTRG.10478.1, MSTRG.232.1 (target gene: ACLY ), ENSSSCT00000086039 (target gene: DGAT2 ), ENSSSCT00000050073, ENSSSCT00000088265, MSTRG.12072.1, MSTRG.14478.1 (target gene: PTGR1 ), ENSSSCT00000073662, ENSSSCT00000075506, ENSSSCT00000087125, and MSTRG.5410.1 (target gene: SREBF1 ), are also closely related to fat metabolism [ 29 , 39 ]. KEGG and GO analyses revealed that many fat and cholesterol metabolism-related pathways and terms were significantly enriched, and some genes related to lipid metabolism were enriched multiple times, including APOA1 , APOA4 , APOB , MTTP , ABCG5 , and ABCG8 [ 40 , 41 , 42 , 43 , 44 ]. Furthermore, via trans prediction, we found that ENSSSCT00000062623, ENSSSCT00000069577, ENSSSCT00000069784, ENSSSCT00000075507, ENSSSCT00000077869, MSTRG.129.2, and MSTRG.5755.1 all formed targeting relationships with APOA1 , APOA4 , APOB , MTTP , ABCG5 , and ABCG8 ( Supplementary File S8 ), and the expression levels of these seven DELs were all higher in the high IMF group, suggesting that these DELs might facilitate lipid transport and metabolism.…”

Haptoglobin and C-Reactive Protein—Non-specific Markers for Nursery Conditions in Swine

“…There are a great number of experimental and field studies confirming the use of APP as indicators for various infectious and inflammatory diseases, but only a few studies used APP to diagnose stress in farm animals. Various studies suggest that relationships between different biomarkers and their precursors and combinations thereof allow a more precise definition of pathological processes than is the case with single markers (19–22). CRP and SAA are the first line rapid reacting APPs increasing within hours.…”