Proteomic analysis on a high salt tolerance introgression strain of Triticum aestivum/Thinopyrum ponticum

Abstract

Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt-tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV-irradiated Agropyron elongatum (Thinopyrum ponticum Podp). We report here a comparative proteomic analysis to investigate variety-specific and salt-responsive proteins between seedling-roots of Shanrong No. 3 and Jinan 177. In total, 114 spots reproducibly presented differential expression patterns on 2-DE maps. Of them, 34 were variety-specific and 49 were salt-responsive. We identified 110 spots by MALDI-TOF MS and partially confirmed by MALDI-TOF-TOF MS, and functionally classified them into signal transduction, transcription and translation, transporting, chaperones, proteolysis and detoxification, etc. Meanwhile, we also found the alteration of protein expression of Shanrong No. 3 through inhibition of old proteins and production of novel ones, change in abundance and sensitivity of some nonsalt-responsive and salt-responsive proteins, as well as PTMs. Furthermore, comparison between proteome and transcripteome using cDNA microarray showed that there were only 20 proteins with abundances correlative to signal densities of corresponding EST probes. This study gives us a global insight into proteomic difference between Shanrong No. 3 and Jinan 177 in constitute and to salt-response.

A Proteomic Study of the Response to Salinity and Drought Stress in an Introgression Strain of Bread Wheat

“…Biomass Measurement and Biochemical Characterization-The measurement of seedling biomass and Na ϩ /K ϩ ratio was performed as described previously (11). The net photosynthesis rate and transpiration rate of the second seedling leaf were assessed using an LI-6400XT portable photosynthesis system (LI-COR Biosciences) under 800 mol⅐m Ϫ2 ⅐s Ϫ1 light and at a temperature of 27°C and a relative humidity of 40%.…”

“…Salinity and Drought Treatments-Wheat seedlings were grown hydroponically following the methods described elsewhere (11). The salinity and drought treatments were applied to seedlings of SR3 and JN177 at the two-leaf stage by adding either 200 mM NaCl or 18% (w/v) polyethylene glycol 6000 to the half-strength Hoagland's culture solution.…”

“…SR3 is a wheat introgression line containing alien chromatin from tall wheatgrass via asymmetric somatic hybridization between parent bread wheat JN177 and its wild relative tall wheatgrass (Thinopyrum ponticum Podp) (9 -11), one of the most salinity-tolerant of all monocotyledonous species (12). The seedling root proteomes of SR3 and JN177 have been compared under both non-stressed and salinity-stressed conditions (11). This comparison led to the identification of 114 differentially expressed proteins (DEPs), and the presumed function of many of these could be defined on the basis of homology with orthologous gene products.…”

Tetracycline Accumulation in Pea Seedlings and its Effects on Proteome and Enzyme Activities

“…These reactions are different than those observed during cold or dry periods (Bogeat-Triboulot et al, 2007;Badowiec et al, 2013). An increase in the expression of ATP synthase was observed in wheat (Wang et al, 2008) and in C4 Aeluropus lagopoides (Poaceae) plants (Sobhanian et al, 2010). A small decrease in expression of ATP synthase may indicate that cellular respiration processes may be disturbed, but expression of NAD-dependent dehydrogenase increased rapidly.…”

“…Along with isoflavone reductase, protective functions against the effects of oxidative stress are played by Hsp 70 -a chaperone protein which intensified synthesis has been found to be caused by high and low temperature, dehydration or excessive salinity (Bae et al, 2003;Wang et al, 2008). A two-fold increase (Fig.…”