Proteomic analysis of wild type and arsenite-resistant Leishmania donovani

Sharma, Shruti; Singh, Gaganmeet; Chavan, Hemantkumar; Dey, Chinmoy Sankar

doi:10.1016/j.exppara.2009.08.003

Cited by 15 publications

(3 citation statements)

References 23 publications

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“…The analysis of cell proteomics is an efficient method to compare protein profiles. Most studies of Leishmania proteomes compared abundance or post-translational modifications (specially phosphorylation) of proteins in amastigotes and promastigotes of the same Leishmania species [ 20 , 22 , 23 , 24 , 25 , 26 ], parasites sensitive and resistant to drugs [ 27 , 28 , 29 , 30 ], and proteins from different Leishmania species [ 31 , 32 ]. Some works also analyzed immunogenic proteins [ 22 , 33 , 34 ] and secreted proteins [ 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Leishmania (L.) amazonensis Protein Expression by Host T Cell Dependent Responses: Differential Expression of Oligopeptidase B, Tryparedoxin Peroxidase and HSP70 Isoforms in Amastigotes Isolated from BALB/c and BALB/c Nude Mice

et al. 2015

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Leishmaniasis is an important disease that affects 12 million people in 88 countries, with 2 million new cases every year. Leishmania amazonensis is an important agent in Brazil, leading to clinical forms varying from localized (LCL) to diffuse cutaneous leishmaniasis (DCL). One interesting issue rarely analyzed is how host immune response affects Leishmania phenotype and virulence. Aiming to study the effect of host immune system on Leishmania proteins we compared proteomes of amastigotes isolated from BALB/c and BALB/c nude mice. The athymic nude mice may resemble patients with diffuse cutaneous leishmaniasis, considered T-cell hyposensitive or anergic to Leishmania´s antigens. This work is the first to compare modifications in amastigotes’ proteomes driven by host immune response. Among the 44 differentially expressed spots, there were proteins related to oxidative/nitrosative stress and proteases. Some correspond to known Leishmania virulence factors such as OPB and tryparedoxin peroxidase. Specific isoforms of these two proteins were increased in parasites from nude mice, suggesting that T cells probably restrain their posttranslational modifications in BALB/c mice. On the other hand, an isoform of HSP70 was increased in amastigotes from BALB/c mice. We believe our study may allow identification of potential virulence factors and ways of regulating their expression.

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Section: Introductionmentioning

confidence: 99%

Regulation of Leishmania (L.) amazonensis Protein Expression by Host T Cell Dependent Responses: Differential Expression of Oligopeptidase B, Tryparedoxin Peroxidase and HSP70 Isoforms in Amastigotes Isolated from BALB/c and BALB/c Nude Mice

et al. 2015

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“…The comparison of proteomes of L. major strains sensitive and resistant to methotrexate, a drug tested for leishmaniasis, indicated that resistant parasites had higher levels of pteridine reductase 1, which converts biopterin in tetra‐hydrobiopterine, an essential factor for Leishmania growth . The comparison of the proteomes of L. donovani wild type and resistant to sodium arsenite identified PTMs in several proteins, and a higher abundance of topoisomerase II in the resistant strain. The comparison of the proteomes of promastigotes of L. infantum attenuated in vitro in the presence of gentamicin indicated alterations in 18 proteins, such as tryparedoxin, TXNPx, peroxidoxin, α‐ and β‐tubulin, and enolase, as well as hypothetical proteins, many of which were related to thiol‐redox control.…”

Section: Potential Applications Of Proteomics In Leishmaniasismentioning

confidence: 99%

Proteomics and Leishmaniasis: Potential Clinical Applications

Capelli-Peixoto

Mule

Tano

et al. 2019

Proteomics Clinical Apps

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Leishmaniases are diseases caused by protozoan parasites of the genus Leishmania. They are endemic in 98 countries, affect around 12 million people worldwide and may present several distinct clinical forms. Unfortunately, there are only a few drugs available for treatment of leishmaniasis, which are toxic and not always effective. Different parasite species and different clinical forms require optimization of the treatment or more specific therapies, which are not available. The emergence of resistance is also a matter of concern. Besides, diagnosis can sometimes be complicated due to atypical manifestations and associations with other pathologies. In this review, proteomic data are presented and discussed in terms of their application in important issues in leishmaniasis such as parasite resistance to chemotherapy, diagnosis of active disease in patients and dogs, markers for different clinical forms, identification of virulence factors, and their potential use in vaccination. It is shown that proteomics has contributed to the discovery of potential biomarkers for prognosis, diagnosis, therapeutics, monitoring of disease progression, treatment follow‐up and identification of vaccine candidates for specific diseases. However, the authors believe its capabilities have not yet been fully explored for routine clinical analysis for several reasons, which will be presented in this review.

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“…Proteomics is less rewarding when studying phenotypic or pathologic diversity. Like transcriptomics, proteomic approaches that studied mechanisms of drug resistance (Sharma et al 2003 ;Drummelsmith et al 2003Drummelsmith et al , 2004Vergnes et al 2007 ;Singh et al 2008 ;Fadili et al 2009) or species diversity (Brobey et al 2006) very often resulted in a list of differentially expressed proteins lacking an annotated function. This again impedes determination of how the different actors relate to each other and often leaves too many open questions.…”

Section: T H E C H a L L E N G E O F L E I S H M A N I A D I V E R S mentioning

confidence: 99%

The potential of metabolomics for Leishmania research in the post-genomics era

et al. 2010

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The post-genomics era has provided researchers with access to a new generation of tools for the global characterization and understanding of pathogen diversity. This review provides a critical summary of published Leishmania post-genomic research efforts to date, and discusses the potential impact of the addition of metabolomics to the post-genomic toolbox. Metabolomics aims at understanding biology by comprehensive metabolite profiling. We present an overview of the design and interpretation of metabolomics experiments in the context of Leishmania research. Sample preparation, measurement techniques, and bioinformatics analysis of the generated complex datasets are discussed in detail. To illustrate the concepts and the expected results of metabolomics analyses, we also present an overview of comparative metabolic profiles of drug-sensitive and drug-resistant Leishmania donovani clinical isolates.

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Proteomic analysis of wild type and arsenite-resistant Leishmania donovani

Cited by 15 publications

References 23 publications

Regulation of Leishmania (L.) amazonensis Protein Expression by Host T Cell Dependent Responses: Differential Expression of Oligopeptidase B, Tryparedoxin Peroxidase and HSP70 Isoforms in Amastigotes Isolated from BALB/c and BALB/c Nude Mice

Regulation of Leishmania (L.) amazonensis Protein Expression by Host T Cell Dependent Responses: Differential Expression of Oligopeptidase B, Tryparedoxin Peroxidase and HSP70 Isoforms in Amastigotes Isolated from BALB/c and BALB/c Nude Mice

Proteomics and Leishmaniasis: Potential Clinical Applications

The potential of metabolomics for Leishmania research in the post-genomics era

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