Proteomic analysis of uterine fluid during the pre-implantation period of pregnancy in cattle

Forde, Niamh; McGettigan, Paul A.; Mehta, Jai Prakash; O'Hara, L.; Mamo, Solomon; Bazer, Fuller W.; Spencer, Thomas E.; Lonergan, P.

doi:10.1530/rep-13-0010

Cited by 114 publications

(103 citation statements)

References 62 publications

(64 reference statements)

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“…By contrast, in another study with the use of bovine OF before fertilization in vitro, it was found that expression of AKR1B1 was affected by the quality of the blastocysts (CebrianSerrano et al 2013). The protein encoded by AKR1B1 has also been found in the uterine luminal fluid of pregnant heifers on Day 16 (Forde et al 2014). In addition, high glucose concentrations in culture media could lead to upregulation of aldose reductase and subsequent accumulation of sorbitol in cytoplasm and activation apoptotic pathways (Wirtu et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Spatial differences in gene expression in the bovine oviduct

et al. 2016

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The aim of this study was to compare the transcriptome of the oviductal isthmus of pregnant heifers with that of cyclic heifers as well as to investigate spatial differences between the transcriptome of the isthmus and ampulla of the oviduct in pregnant heifers. After synchronizing crossbred beef heifers, those in standing oestrus (=Day 0) were randomly assigned to cyclic (non-bred, n = 6) or pregnant (artificially inseminated, n = 11) groups. They were slaughtered on Day 3 and both oviducts from each animal were isolated and cut in half to separate ampulla and isthmus. Each portion was flushed to confirm the presence of an oocyte/embryo and was then opened longitudinally and scraped to obtain epithelial cells which were snap-frozen. Oocytes and embryos were located in the isthmus of the oviduct ipsilateral to the corpus luteum. Microarray analysis of oviductal cells revealed that proximity to the corpus luteum did not affect the transcriptome of the isthmus, irrespective of pregnancy status. However, 2287 genes were differentially expressed (P < 0.01) between the ampulla and isthmus of the oviduct ipsilateral to the corpus luteum in pregnant animals. Gene ontology revealed that the main biological processes overrepresented in the isthmus were synthesis of nitrogen, lipids, nucleotides, steroids and cholesterol as well as vesicle-mediated transport, cell cycle, apoptosis, endocytosis and exocytosis, whereas cell motion, motility and migration, DNA repair, calcium ion homeostasis, carbohydrate biosynthesis, and regulation of cilium movement and beat frequency were overrepresented in the ampulla. In conclusion, large differences in gene expression were observed between the isthmus and ampulla of pregnant animals at Day 3 after oestrus.Reproduction (2016) 152 37-46

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Section: Discussionmentioning

confidence: 99%

Spatial differences in gene expression in the bovine oviduct

et al. 2016

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“…Briefly, we were able to demonstrate that BSA, purine nucleoside phosphorylase (PNP), and heat shock 70 kDa protein 5 (HSPA5) showed a type 1, type 3, and type 3 validation profile respectively (Muñ oz et al 2012). The cow endometrium predominantly transcribes PNP (Forde et al 2014). Unaltered PNP and HSP70 levels indicated no changes in purine metabolism and no stress resulting from cytotoxic damage.…”

Section: Response Models In the Uterine Fluid And Endometriummentioning

confidence: 99%

Multiple-embryo transfer for studying very early maternal–embryo interactions in cattle

Gómez

Muñoz

2015

Reproduction

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In the present paper, we highlight the need to study very early maternal-embryo interactions and discuss how these interactions can be addressed. Bovine species normally carry one or, less frequently, two embryos to term; there are very rare cases of triplets or higher-order multiple pregnancies in which all the offspring are born alive. Multiple-embryo transfer (MET) in cattle allows for the detection of endometrial responses in scenarios where single-embryo transfer would not. Although MET is non-physiological, the present study shows that at the very early embryonic stages, a uterus carrying zona-enclosed embryos does not exhibit non-physiological reactions. On the contrary, MET should be considered the sum of multiple individual effects triggered by developing embryos. We provide arguments to support our hypothesis that describe a rationale for current work with MET, and we discuss alternative hypotheses. Using cattle as a model, we describe how technical approaches to analyzing zona-enclosed early embryo-maternal interactions (i.e., transcriptomics, proteomics, and endometrial cell culture) can help identify molecular changes that may be difficult to observe when only a single embryo is present. We conclude that MET can be used for studying very early maternal-embryo interactions in vivo in monotocous species. Free Spanish abstract: A Spanish translation of this abstract is freely available at http://www.reproduction-online.org/content/150/2/ R35/suppl/DC1.Reproduction (2015) 150 R35-R43

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“…The histotroph consists of different substances (e.g. amino acids, carbohydrates, proteins, lipids) transported into the uterine lumen by endometrial epithelial cells from the blood and as specific secretory products encoded by genes expressed in the endometrial epithelium (Bazer, 1975;Gray et al, 2001;Forde et al, 2014). Embryo development in uterine-gland knock-out sheep has shown to be retarded from day 9-14, indicating the importance of the endometrial epithelial secretions (Gray et al, 2002).…”

Section: Preparations In the Embryo Recipientmentioning

confidence: 99%

Basic and practical aspects of pregnancy establishment in cattle

Pedersen¹,

Mazzoni²,

Stroebech³

et al. 2017

Anim Reprod

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Bovine embryos are increasingly produced using reproductive technologies, e.g. ovum pick-up (OPU), in vitro embryo production (IVP) and embryo transfer (ET). Such in vitro manipulated embryos are known to deviate in several aspects compared to in vivo derived embryos. Pregnancy establishment in cattle involves timed biological events including fine-tuned communication, initiated and carried out by both the embryo and the endometrium. This stimulates research to increase the understanding of events and interactions taking place in the uterus after embryo transfer, both from a biological and systems biology point of view. This review will focus on the biological events taking place during early embryonic development, implantation and beginning of placentation, with focus on transfer of in vitro produced embryos, including a systems biology approach for selection of superior embryo recipients.

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Proteomic analysis of uterine fluid during the pre-implantation period of pregnancy in cattle

Cited by 114 publications

References 62 publications

Spatial differences in gene expression in the bovine oviduct

Spatial differences in gene expression in the bovine oviduct

Multiple-embryo transfer for studying very early maternal–embryo interactions in cattle

Basic and practical aspects of pregnancy establishment in cattle

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