Proteomic analysis of urinary and tissue‐exudative extracellular vesicles to discover novel bladder cancer biomarkers

Tomiyama, Eisuke; Matsuzaki, Kyosuke; Fujita, Kazutoshi; Shiromizu, Takashi; Narumi, Ryohei; Jingushi, Kentaro; Koh, Yoko; Matsushita, Makoto; Nakano, Kosuke; Hayashi, Yujiro; Wang, Cong; Ishizuya, Yu; Kato, Taigo; Hatano, Koji; Kawashima, Atsunari; Ujike, Takeshi; Uemura, Mamoru; Takao, Tetsuya; Adachi, Jun; Tomonaga, Takeshi; Nonomura, Norio

doi:10.1111/cas.14881

Cited by 38 publications

(40 citation statements)

References 58 publications

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“…Another approach is the targeted proteomic analysis, in which only specific proteins are detected and quantified [329]. The most common method is the multiplexed multiple reaction monitoring (MRM), which is often used as a method for validation of biomarkers detected in MS [330].…”

Section: Methods For the Study Of Exosomal Contentmentioning

confidence: 99%

Exosomes: Potential Disease Biomarkers and New Therapeutic Targets

et al. 2021

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Exosomes are extracellular vesicles released by cells, both constitutively and after cell activation, and are present in different types of biological fluid. Exosomes are involved in the pathogenesis of diseases, such as cancer, neurodegenerative diseases, pregnancy disorders and cardiovascular diseases, and have emerged as potential non-invasive biomarkers for the detection, prognosis and therapeutics of a myriad of diseases. In this review, we describe recent advances related to the regulatory mechanisms of exosome biogenesis, release and molecular composition, as well as their role in health and disease, and their potential use as disease biomarkers and therapeutic targets. In addition, the advantages and disadvantages of their main isolation methods, characterization and cargo analysis, as well as the experimental methods used for exosome-mediated drug delivery, are discussed. Finally, we present potential perspectives for the use of exosomes in future clinical practice.

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Section: Methods For the Study Of Exosomal Contentmentioning

confidence: 99%

Exosomes: Potential Disease Biomarkers and New Therapeutic Targets

et al. 2021

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“…However, urine contains numerous EVs derived not only from the bladder but also from the kidney and normal urothelial epithelium (which may falsify the results). Researchers in a study [ 29 ] attempted to isolate tissue exudate EVs (Te-EVs) from freshly excised live BCa tissue from a culture medium and analyse them proteomic along with EVs from urine. In this way, more reliable BCa biomarkers with high specificity and sensitivity were obtained.…”

Section: What Is New In the Last Two Years?mentioning

confidence: 99%

A Liquid Biopsy in Bladder Cancer—The Current Landscape in Urinary Biomarkers

Matuszczak

Kiljańczyk

Salagierski

2022

IJMS

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The non-muscle invasive bladder cancer tends to recur and progress. Therefore, it requires frequent follow-ups, generating costs and making it one of the most expensive neoplasms. Considering the expensive and invasive character of the current gold-standard diagnostic procedure, white-light cystoscopy, efforts to find an alternative method are ongoing. Although the last decade has seen significant advancements in urinary biomarker tests (UBTs) for bladder cancer, international guidelines have not recommended them. Currently, the paramount urgency is to find and validate the test with the best specificity and sensitivity, which would allow for the optimizing of diagnosis, prognosis, and a treatment plan. This review aims to summarise the up-to-date state of knowledge relating to UBTs and new developments in the detection, prognosis, and surveillance of bladder cancer and their potential applications in clinical practice.

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“…cerebrospinal fluid [131], urine [132][133][134], and formalin-fixed paraffinembedded (FFPE) samples [135][136][137]. These analyses have sought to quantify the protein biomarkers of breast cancer [21,[137][138][139][140], colorectal cancer [141][142][143][144][145], lung cancer [146][147][148][149], oral cancer [150,151], multiple sclerosis [131,152], cardiovascular diseases [153][154][155][156][157][158],…”

Section: Biomedical Research and Clinical Practicementioning

confidence: 99%

Targeted liquid chromatography‐tandem mass spectrometry analysis of proteins: Basic principles, applications, and perspectives

2021

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Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is now the main analytical method for the identification and quantification of peptides and proteins in biological samples. In modern research, identification of biomarkers and their quantitative comparison between samples are becoming increasingly important for discovery, validation, and monitoring. Such data can be obtained following specific signals after fragmentation of peptides using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) methods, with high specificity, accuracy, and reproducibility. In addition, these methods allow measurement of the amount of posttranslationally modified forms and isoforms of proteins. This review article describes the basic principles of MRM assays, guidelines for sample preparation, recent advanced MRM-based strategies, applications and illustrative perspectives of MRM/PRM methods in clinical research and molecular biology.

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Proteomic analysis of urinary and tissue‐exudative extracellular vesicles to discover novel bladder cancer biomarkers

Cited by 38 publications

References 58 publications

Exosomes: Potential Disease Biomarkers and New Therapeutic Targets

Exosomes: Potential Disease Biomarkers and New Therapeutic Targets

A Liquid Biopsy in Bladder Cancer—The Current Landscape in Urinary Biomarkers

Targeted liquid chromatography‐tandem mass spectrometry analysis of proteins: Basic principles, applications, and perspectives

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