Proteomic Analysis of Upland Rice (Oryza sativa L.) Exposed to Intermittent Water Deficit

Abstract

Rice is the most important crop consumed all over the world. In Brazil, irrigated rice covers 50 % of the rice producing area and is responsible for 75 % of the national production. Upland rice covers most of the remaining area, and is therefore, a very important production system in the country. In the present study, we have used the drought tolerant upland rice variety Três Meses Antigo to investigate the proteomic changes that occur during drought stress. Plants were submitted to drought by the reposition of 50 % of the water lost daily. Twenty days after the beginning of the drought stress period, leaves were harvested and used for protein extraction. The 2D maps obtained from treated and control plants revealed 408 reproducible spots, 44 of which were identified by mass spectrometry, including 15 differential proteins. Several unaltered proteins were also identified (39 spots) and were mainly involved in photosynthesis. Taken together, the results obtained suggest that the tolerant upland rice up-regulates anti-oxidant and energy production related proteins in order to cope with water deficit.

Wheat Line “RYNO3936” Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery

“…Changes in the transcriptome differs to changes at protein level ( Gygi et al., 1999 ; Bogeat-Triboulot et al., 2006 ; Morimoto and Yahara, 2018 ), necessitating studies into the proteome to elucidate the water stress response pathway in crop species ( Ford et al., 2011 ; Budak et al., 2013 ; Rabello et al., 2014 ; Liu et al., 2015 ; Chmielewska et al., 2016 ). In our study, we also found that our mutant RYNO3936 expressed proteins associated with osmotic stress tolerance (e.g.…”

Improving drought tolerance in rice: Ensuring food security through multi‐dimensional approaches

“…As OsPP2C is a negative approach. This study led to the identification of 15 differential proteins of which seven showed increased abundance including an APX and a GSH-dependent dehydroascorbate reductase 1 (Rabello et al, 2014).…”

“…There are several proteins associated with the biosynthesis of plant hormones, their signalling pathways and drought tolerance (Rabello et al ., 2014). Owing to the low abundance of the hormone‐responsive proteins, it is relatively difficult to identify the proteins related to the hormone biosynthesis and signalling using a gel‐based proteomics approach.…”

“…regulator of ABA-signalling in plants(Gosti et al, 1999;Gupta et al, 2018), downregulation of this protein in response to drought stress suggests positive regulation of ABA in drought stress tolerance in rice(Gupta et al, 2019).In order to elucidate the effect of intermittent drought stress on rice leaves,Rabello et al (2014) utilised a 2-DGE-based proteomic…”

Cereal Crop Proteomics: Systemic Analysis of Crop Drought Stress Responses Towards Marker-Assisted Selection Breeding

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